FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004689734

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689734
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1530406
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCA78870.515353442158486No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC73530.48046074048324433No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAAGCGCTGCTGTGCCAAATCATACAATAATAGTCAG70560.46105412550656494No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG64930.42426650183023334No Hit
GTGCTGACTCAGCCGTCTTCCCTCTCTGCATCTCCTGGAGCATCAGCCAG55090.35996983806911365No Hit
CTCCAGAGCCCTGCTGCTTATCTGAGTCTGATTTGTACCTCAGGAGATATTGGGGAGGACTCCCTGGCCTCTG53930.35239015006475405No Hit
CACCTGCACCTTACGCAGTGGCATCAATGTTGGTACCTACAGGATATACTGGTATCAGCAGAGGCCAGGGAGTC50660.3310232709490161No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC48450.3165826584579517No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG47910.31305418300764637No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG46970.3069120220385963No Hit
GGCCTGGACTCCTCTCCTCCTCCTGTTCCTCTCTCACTGCACAGGTTCCC46470.30364491514016545No Hit
GCTCTGGAGTCCCCAGCCGCTTCTCTGGATCCAAAGATGCTTCGGCCAATGCAGGGATTTTACTCATCTCTGGG45540.297568096309084No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT44710.29214469885768873No Hit
GGTTCCCTCTCGCAGGCTGTGCTGACTCAGCCGTCTTCCCTCTCTGCATCTCCTGGAGCATCAGCCAGTCTCAC40660.26568113298039864No Hit
CCCCAATATCTCCTGAGGTACAAATCAGACTCAGATAAGCAGCAGGGCTC40380.26385155311727737No Hit
GGATATACTGGTATCAGCAGAGGCCAGGGAGTCCTCCCCAATATCTCCTG40380.26385155311727737No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC39120.25561844373323156No Hit
GTACGGGACTGCGGGGGTAAGAGGTTGTGTCCACCATGGCCTGGACTCCTCTCCTCCTCCTGTTCCTCTCTCA38230.24980299345402462No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG38130.24914957207433844No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG38070.24875751924652673No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT37630.24588246517590756No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACAC37400.24437959600262937No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA37340.24398754317481763No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG36970.24156988406997879No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG36890.24104714696622986No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC36350.2375186715159245No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC36180.23640785517045804No Hit
CTCGCAGGCTGTGCTGACTCAGCCGTCTTCCCTCTCTGCATCTCCTGGAG36050.235558407376866No Hit
GGTAAGAGGTTGTGTCCACCATGGCCTGGACTCCTCTCCTCCTCCTGTTCCTCTCTCACTGCACAGGTTCCCTCT36010.23529703882499156No Hit
GGCCAGGGAGTCCTCCCCAATATCTCCTGAGGTACAAATCAGACTCAGAT35530.2321606162024979No Hit
TCCTCAGACTGGAGCCCAGAGATGAGTAAAATCCCTGCATTGGCCGAAGC34530.22562640240563614No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA34220.223600796128609No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAAGCGCTGCTGTGCCAAATCATACAATAATAGTCAGC34080.22268600619704837No Hit
CAATAATAGTCAGCCTCATCCTCAGACTGGAGCCCAGAGATGAGTAAAAT33950.2218365584034563No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG32650.21334208046753605No Hit
ATGTTGGTACCTACAGGATATACTGGTATCAGCAGAGGCCAGGGAGTCCTCCCCAATATCTCCTGAGGTACAAAT32260.21079373708676No Hit
TCTCTGGGCTCCAGTCTGAGGATGAGGCTGACTATTATTGTATGATTTGG32150.21007497356910518No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT32070.20955223646535623No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA31740.20739594591239188No Hit
CTATTATTGTATGATTTGGCACAGCAGCGCTTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCC30820.20138446921927908No Hit
GTCTGAGGATGAGGCTGACTATTATTGTATGATTTGGCACAGCAGCGCTTGGGTGTTCGGCGGAGGGACCAAG30230.19752928307913065No Hit
ATAATAGTCAGCCTCATCCTCAGACTGGAGCCCAGAGATGAGTAAAATCC29750.194392860456637No Hit
CTACAGGATATACTGGTATCAGCAGAGGCCAGGGAGTCCTCCCCAATATC28960.18923083155711623No Hit
CCTCTCCTCCTCCTGTTCCTCTCTCACTGCACAGGTTCCCTCTCGCAGGCTGTGCTGACTCAGCCGTCTTCCCT28590.18681317245227735No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG28010.18302332845009756No Hit
ATATTGGGGAGGACTCCCTGGCCTCTGCTGATACCAGTATATCCTGTAGG27990.18289264417416032No Hit
CCCAAGCGCTGCTGTGCCAAATCATACAATAATAGTCAGCCTCATCCTCA27460.1794295108618236No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA27180.1775999309987023No Hit
GTATCAACGCAGAGTACGGGACTGCGGGGGTAAGAGGTTGTGTCCACCATGGCCTGGACTCCTCTCCTCCTC27100.17707719389495336No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC27010.1764891146532358No Hit
CTCACTGCACAGGTTCCCTCTCGCAGGCTGTGCTGACTCAGCCGTCTTCCCTCTCTGCATCTCCTGGAGCATCAG26720.1745941926521459No Hit
ATCCTGTAGGTACCAACATTGATGCCACTGCGTAAGGTGCAGGTGAGACT26620.17394077127245974No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT26550.1734833763066794No Hit
GAGTACGGGACTGCGGGGGTAAGAGGTTGTGTCCACCATGGCCTGGACTCCTCTCCTCCTCCTGTTCCTCTCTCA26470.17296063920293048No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA26370.1723072178232443No Hit
CAGATAAGCAGCAGGGCTCTGGAGTCCCCAGCCGCTTCTCTGGATCCAAA25930.16943216375262513No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC25040.16361671347341816No Hit
CTCCCTGGCCTCTGCTGATACCAGTATATCCTGTAGGTACCAACATTGATGCCACTGCGTAAGGTGCAGGTGAGA24720.1615257650584224No Hit
GCACAGGTTCCCTCTCGCAGGCTGTGCTGACTCAGCCGTCTTCCCTCTCTGCATCTCCTGGAGCATCAGCCAGT24710.1614604229204538No Hit
GACTATTATTGTATGATTTGGCACAGCAGCGCTTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAG24390.15936947450545802No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC24110.15753989464233675No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT23740.15512223553749788No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC23460.1532926556743766No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAAGCGCTG23350.15257389215672182No Hit
GCTTGGTCCCTCCGCCGAACACCCAAGCGCTGCTGTGCCAAATCATACAATAATAGTCAGCCTCATCCTCAGA23050.1506136280176633No Hit
CTGCACAGGTTCCCTCTCGCAGGCTGTGCTGACTCAGCCGTCTTCCCTCT22690.14826131105079304No Hit
GTGCAGGTGAGACTGGCTGATGCTCCAGGAGATGCAGAGAGGGAAGACGG22670.14813062677485583No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC22500.14701981042938933No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG22050.14407941422080153No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA21890.14303394001330366No Hit
GTACAAATCAGACTCAGATAAGCAGCAGGGCTCTGGAGTCCCCAGCCGCTTCTCTGGATCCAAAGATGCTTCGGC21850.1427725714614292No Hit
GATTTGTACCTCAGGAGATATTGGGGAGGACTCCCTGGCCTCTGCTGATA21710.14185778152986855No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC21590.14107367587424513No Hit
GTCTCACCTGCACCTTACGCAGTGGCATCAATGTTGGTACCTACAGGATA21400.13983217525284142No Hit
ATGCAGGGATTTTACTCATCTCTGGGCTCCAGTCTGAGGATGAGGCTGAC21020.13734917401003394No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG20700.13525822559503817No Hit
GGGCTCTGGAGTCCCCAGCCGCTTCTCTGGATCCAAAGATGCTTCGGCCA20420.1334286457319169No Hit
CTCCTGTTCCTCTCTCACTGCACAGGTTCCCTCTCGCAGGCTGTGCTGACTCAGCCGTCTTCCCTCTCTGCATCT20390.13323261931801103No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT20290.13257919793832484No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT20210.13205646083457592No Hit
GGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACC20100.13133769731692113No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC20020.13081496021317218No Hit
GCTTATCTGAGTCTGATTTGTACCTCAGGAGATATTGGGGAGGACTCCCTGGCCTCTGCTGATACCAGTATATC19950.13035756524739187No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA19920.13016153883348602No Hit
GCCTCATCCTCAGACTGGAGCCCAGAGATGAGTAAAATCCCTGCATTGGCCGAAGCATCTTTGGATCCAGAGAAG19710.12878935393614505No Hit
CTCCTCTCCTCCTCCTGTTCCTCTCTCACTGCACAGGTTCCCTCTCGCAGGCTGTGCTGACTCAGCCGTCTTCC19580.12793990614255304No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGC19270.12591429986552588No Hit
GGGGTAAGAGGTTGTGTCCACCATGGCCTGGACTCCTCTCCTCCTCCTGTTCCTCTCTCACTGCACAGGTTCCCT19100.12480348352005939No Hit
CTCCTCCTCCTGTTCCTCTCTCACTGCACAGGTTCCCTCTCGCAGGCTGTGCTGACTCAGCCGTCTTCCCTCT19010.12421540427834182No Hit
GCCCAGAGATGAGTAAAATCCCTGCATTGGCCGAAGCATCTTTGGATCCAGAGAAGCGGCTGGGGACTCCAGAGC18970.12395403572646735No Hit
GGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG18630.12173240303553436No Hit
GTCCACCATGGCCTGGACTCCTCTCCTCCTCCTGTTCCTCTCTCACTGCACAGGTTCCCTCTCGCAGGCTGTG18390.12016419172428755No Hit
GCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGC17920.11709311123976252No Hit
GGACTCCTCTCCTCCTCCTGTTCCTCTCTCACTGCACAGGTTCCCTCTCGCAGGCTGTGCTGACTCAGCCGTCTT17880.11683174268788804No Hit
GATTTTACTCATCTCTGGGCTCCAGTCTGAGGATGAGGCTGACTATTATT17860.11670105841195082No Hit
GGATGAGGCTGACTATTATTGTATGATTTGGCACAGCAGCGCTTGGGTGTTCGGCGGAGGGACCAAGCTGACCGT17810.11637434772210772No Hit
CTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCC17440.11395668861726889No Hit
GCGCTTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCC17220.1125191615819593No Hit
GGTATCAGCAGAGGCCAGGGAGTCCTCCCCAATATCTCCTGAGGTACAAATCAGACTCAGATAAGCAGCAGGGCT17000.11108163454664971No Hit
TCTTTGGATCCAGAGAAGCGGCTGGGGACTCCAGAGCCCTGCTGCTTATC16950.11075492385680663No Hit
CTGCATCTCCTGGAGCATCAGCCAGTCTCACCTGCACCTTACGCAGTGGC16870.11023218675305768No Hit
GTGCCAAATCATACAATAATAGTCAGCCTCATCCTCAGACTGGAGCCCAGAGATGAGTAAAATCCCTGCATTGGC16870.11023218675305768No Hit
ATACTGGTATCAGCAGAGGCCAGGGAGTCCTCCCCAATATCTCCTGAGGT16750.10944808109743427No Hit
CTACAGGATATACTGGTATCAGCAGAGGCCAGGGAGTCCTCCCCAATATCTCCTGAGGTACAAATCAGACTCAGA16650.10879465971774811No Hit
GTATATCCTGTAGGTACCAACATTGATGCCACTGCGTAAGGTGCAGGTGA16490.10774918551025021No Hit
GTTGTGTCCACCATGGCCTGGACTCCTCTCCTCCTCCTGTTCCTCTCTCACTGCACAGGTTCCCTCTCGCAGG16490.10774918551025021No Hit
ACCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAAAAGCTACAGCTGC16380.10703042199259542No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC16380.10703042199259542No Hit
GGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTG16340.10676905344072095No Hit
CGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC16100.10520084212947414No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGG15920.10402468364603902No Hit
GTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTT15850.10356728868025869No Hit
GGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAG15830.10343660440432147No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC15800.10324057799041561No Hit
GGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAAC15670.1023911301968236No Hit
GAGGTTGTGTCCACCATGGCCTGGACTCCTCTCCTCCTCCTGTTCCTCTC15540.10154168240323155No Hit
GTCTTCCCTCTCTGCATCTCCTGGAGCATCAGCCAGTCTCACCTGCACCTTACGCAGTGGCATCAATGTTGGTAC15480.10114962957541987No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG15480.10114962957541987No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)