FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004689735

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689735
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences242905
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATTATAGTCCTCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAGACGAACTGTGGCTGCACCAT13800.5681233404005681No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT10110.41621209938041626No Hit
CTCCTAACCTGCTCATTTACTGGGCATCTGCCCGGGAGTCCGGGGTCCCT9530.39233445174039233No Hit
GGTTAGGAGGCTGTCCTGGTTTTTGCTGGTACCAAGCTAAGTAGTTCTTACTGGTGGAGCTGAATAAAAGACT8260.34005063708034006No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC8100.33346369980033347No Hit
CTTATACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGT7390.30423416562030425No Hit
CTATAATATTGCTGACAGTAATAAACGGCCACATCTTCAGCCTGCAGGCTGTTGATGGTGAGAGTGAAATCTGTC6850.282003252300282No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC6690.2754163150202754No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG6670.2745929478602746No Hit
CTTTTATTCAGCTCCACCAGTAAGAACTACTTAGCTTGGTACCAGCAAAA5990.2465984644202466No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT5330.2194273481402194No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG4990.20543010642020543No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG4880.2009015870402009No Hit
TTATAGTCCTCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAGACGAACTGTGGCTGCACCATCTGTCTT4680.19266791544019268No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG4560.18772771248018771No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG4400.18114077520018115No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT4250.17496552150017497No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC4170.17167205286017168No Hit
GTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCAC3990.16426174842016425No Hit
GATTTCACTCTCACCATCAACAGCCTGCAGGCTGAAGATGTGGCCGTTTATTACTGTCAGCAATATTATAGTCCT3960.16302669768016304No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTCTTTTATT3900.16055659620016055No Hit
CTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTCTTTTATTCAGCTCCAC3860.1589098618801589No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA3860.1589098618801589No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC3800.15643976040015645No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT3740.15396965892015396No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT3730.15355797534015356No Hit
GAACTACTTAGCTTGGTACCAGCAAAAACCAGGACAGCCTCCTAACCTGC3710.15273460818015272No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG3490.14367756942014367No Hit
CCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGC3480.14326588584014327No Hit
GTAATACACGGCTGAGTCCTCGGTTTCCAGGCTGTTCATTTGCAGATAAA3450.14203083510014203No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA3450.14203083510014203No Hit
ATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAACAGCC3430.1412074679401412No Hit
GTTTATTACTGTCAGCAATATTATAGTCCTCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAGACGAA3340.1375023157201375No Hit
AACCAGGACAGCCTCCTAACCTGCTCATTTACTGGGCATCTGCCCGGGAG3270.1346205306601346No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT3260.1342088470801342No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC3230.13297379634013298No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC3180.1309153784401309No Hit
CCCTTAGACTCTCCTGTGTAGGATCTGGACTCACTTTCGCTAACGCCTGG3120.12844527696012845No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG3110.12803359338012804No Hit
CACCATCAACAGCCTGCAGGCTGAAGATGTGGCCGTTTATTACTGTCAGCAATATTATAGTCCTCCGTGGACGTT3050.12556349190012556No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT3030.12474012474012475No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA3000.1235050740001235No Hit
GGACTATAATATTGCTGACAGTAATAAACGGCCACATCTTCAGCCTGCAGGCTGTTGATGGTGAGAGTGAAAT2980.12268170684012268No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA2940.12103497252012103No Hit
GTGCAGCCACAGTTCGTCTGATTTCCACCTTGGTCCCTTGGCCGAACGTC2910.1197999217801198No Hit
GAATAAAAGACTCTGGCTGGACTTGCAGTTGATGGTGGCCCTCTCGCCCAGAGACACAGCCAGGGAGTCTGGAGA2870.11815318746011816No Hit
GTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTCTTTTATTCAGCTCCACCAGTAAGAACTA2840.11691813672011693No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC2760.11362466808011362No Hit
GTCAGGGACCCCGGACTCCCGGGCAGATGCCCAGTAAATGAGCAGGTTAGGAGGCTGTCCTGGTTTTTGCTGGTA2730.11238961734011238No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA2730.11238961734011238No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG2730.11238961734011238No Hit
GCTCATTTACTGGGCATCTGCCCGGGAGTCCGGGGTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTT2710.11156625018011157No Hit
AGTAAGAACTACTTAGCTTGGTACCAGCAAAAACCAGGACAGCCTCCTAACCTGCTCATTTACTGGGCATCTGCC2670.10991951586010991No Hit
CTACTTAGCTTGGTACCAGCAAAAACCAGGACAGCCTCCTAACCTGCTCATTTACTGGGCATCTGCCCGGGAGTC2670.10991951586010991No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG2630.10827278154010828No Hit
GCCCGGGAGTCCGGGGTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAACAGC2590.10662604722010663No Hit
GGACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGATTTTC2580.10621436364010621No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG2580.10621436364010621No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA2570.1058026800601058No Hit
CCATCAACAGCCTGCAGGCTGAAGATGTGGCCGTTTATTACTGTCAGCAA2570.1058026800601058No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT2560.1053909964801054No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA2510.10333257858010332No Hit
TTATTACTGTCAGCAATATTATAGTCCTCCGTGGACGTTCGGCCAAGGGA2500.10292089500010292No Hit
GATTCACTTTATTCATTCTCCTCCAACATTAGCATAATTAAAGCCAAGGA2480.10209752784010209No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC2450.10086247710010085No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG2450.10086247710010085No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT2430.10003910994010004No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)