FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689736

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689736
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences242905
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG36801.5149955744015149No Hit
ATATTATAGTCCTCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAGACGAACTGTGGCTGCACCAT14520.5977645581605978No Hit
CTATAATATTGCTGACAGTAATAAACGGCCACATCTTCAGCCTGCAGGCTGTTGATGGTGAGAGTGAAATCTGTC10310.4244457709804244No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT10100.4158004158004158No Hit
CTCCTAACCTGCTCATTTACTGGGCATCTGCCCGGGAGTCCGGGGTCCCT7900.32523002820032526No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC6210.25565550318025565No Hit
GGTTAGGAGGCTGTCCTGGTTTTTGCTGGTACCAAGCTAAGTAGTTCTTACTGGTGGAGCTGAATAAAAGACT6090.2507153002202507No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG6050.2490685659002491No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC5950.24495173010024496No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC5610.23095448838023097No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT5420.22313250036022314No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT4680.19266791544019268No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG4550.18731602890018734No Hit
TTATAGTCCTCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAGACGAACTGTGGCTGCACCATCTGTCTT4490.18484592742018485No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA4260.17537720508017537No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT4140.17043700212017043No Hit
CTTTTATTCAGCTCCACCAGTAAGAACTACTTAGCTTGGTACCAGCAAAA4100.1687902678001688No Hit
GGACTATAATATTGCTGACAGTAATAAACGGCCACATCTTCAGCCTGCAGGCTGTTGATGGTGAGAGTGAAAT3900.16055659620016055No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG3890.16014491262016015No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG3810.15685144398015685No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT3760.1547930260801548No Hit
GTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCAC3760.1547930260801548No Hit
GTAATACACGGCTGAGTCCTCGGTTTCCAGGCTGTTCATTTGCAGATAAA3760.1547930260801548No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA3720.15314629176015312No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC3700.15232292460015232No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG3580.14738272164014737No Hit
GATTTCACTCTCACCATCAACAGCCTGCAGGCTGAAGATGTGGCCGTTTATTACTGTCAGCAATATTATAGTCCT3470.14285420226014287No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC3330.1370906321401371No Hit
GTAATAAACGGCCACATCTTCAGCCTGCAGGCTGTTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCA3300.13585558140013584No Hit
CTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTCTTTTATTCAGCTCCAC3270.1346205306601346No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTCTTTTATT3250.1337971635001338No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT3220.13256211276013258No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG3180.1309153784401309No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC3180.1309153784401309No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA3140.12926864412012926No Hit
CCCTTAGACTCTCCTGTGTAGGATCTGGACTCACTTTCGCTAACGCCTGG3060.125975175480126No Hit
GTGCAGCCACAGTTCGTCTGATTTCCACCTTGGTCCCTTGGCCGAACGTC3020.12432844116012431No Hit
CACCATCAACAGCCTGCAGGCTGAAGATGTGGCCGTTTATTACTGTCAGCAATATTATAGTCCTCCGTGGACGTT3020.12432844116012431No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA3010.12391675758012391No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT2990.12309339042012309No Hit
TCTTCAGCCTGCAGGCTGTTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACC2950.12144665610012144No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT2950.12144665610012144No Hit
GTTTATTACTGTCAGCAATATTATAGTCCTCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAGACGAA2950.12144665610012144No Hit
GAGTGAAATCTGTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACCCCG2940.12103497252012103No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT2830.1165064531401165No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA2790.11485971882011486No Hit
TTATTACTGTCAGCAATATTATAGTCCTCCGTGGACGTTCGGCCAAGGGA2770.11403635166011403No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG2770.11403635166011403No Hit
CCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGC2760.11362466808011362No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA2750.11321298450011322No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA2720.11197793376011198No Hit
AACCAGGACAGCCTCCTAACCTGCTCATTTACTGGGCATCTGCCCGGGAG2690.11074288302011075No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC2690.11074288302011075No Hit
GTTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCACTGAATCGGT2680.11033119944011034No Hit
ATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAACAGCC2640.10868446512010868No Hit
GTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACCCCGGACTCCCGGGC2580.10621436364010621No Hit
GTATCAACGCAGAGTACGGGGAGCTACAACAGGCAGGCAGGGGCAGCAAG2580.10621436364010621No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC2570.1058026800601058No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT2540.10456762932010456No Hit
GCCCGGGAGTCCGGGGTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAACAGC2480.10209752784010209No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG2470.10168584426010169No Hit
GAACTACTTAGCTTGGTACCAGCAAAAACCAGGACAGCCTCCTAACCTGC2440.10045079352010045No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGATGG200.005862612754.79711563
TCCCGAT200.005988545354.50362861
TTCCGTG200.00824310650.2739312
GTACGGA405.897777E-541.920831
GTATCAA6150.041.4436841
ACCTTAA350.001538865438.36712615
TTGACTA350.001546679238.3276149
AGGCTTG350.001546679238.3276145
GGCTTGA451.1805191E-437.262966
TACGGAG451.18338554E-437.2476162
TATCAAC7300.034.9005052
ATCAACG7400.034.428883
TCAACGC7400.033.989864
CAACGCA7500.033.9838185
AACGCAG7450.033.761746
CTGGGAA400.002978406633.5366634
ATTAGAG502.1985674E-433.5228543
CTAAGCC400.002984427133.52285413
TATTAGA502.1985674E-433.5228542
CTAATAC400.002984427133.5228543
Produced by FastQC (version 0.11.5)