FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004689737

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689737
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences605669
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATGTGGTGACTCAGCCACCCTCGATGTCAGTGGCCCCAGGACAGACG26580.4388535652311741No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT25010.4129318158928391No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTAACCTTGGTCCCAG23360.3856892130850349No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG21440.35398872981777174No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC21270.3511819161951495No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC20770.3429265820109664No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC18650.30792396507003No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC15640.25822685328124767No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT14830.24485321190287102No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC14640.24171618491288147No Hit
CCCCAGGACAGACGGCCCGGATTACCTGTGGGGGAAACAACATTGAAAGG14100.2328004239939637No Hit
GCCTTGGGCTGACCTAGGACGGTAACCTTGGTCCCAGCTCCAAAGACATAATGATCACTACCACCATCCCACAC14030.23164467720817808No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT13940.23015871705502508No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT13320.21992210266663803No Hit
CTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGT13310.2197569959829544No Hit
GACCTAGGACGGTAACCTTGGTCCCAGCTCCAAAGACATAATGATCACTA13090.21612464894191383No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTAACCTTGGTCCCAGCTCCAAAGACATAATGATCA13010.21480379547244452No Hit
CTATTACTGTCAGGTGTGGGATGGTGGTAGTGATCATTATGTCTTTGGAG12700.20968548827825098No Hit
GCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTGTCACTGCACAGGCTCTGTGACCTCTTATGTGGTGACT12580.20770420807404705No Hit
GTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGC11850.1956514201651397No Hit
CGGCCACCCTGACCATCAGCAGGGTCGAAGCCGGGGATGAGGCCGACTAT11710.19333992659356844No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG11390.18805651271569124No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC11350.1873960859809566No Hit
GCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTGTCACTGCACAGGCTCTGTGA11350.1873960859809566No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG10960.18095692531729377No Hit
GAGTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGG10890.17980117853150815No Hit
GTATCAACGCAGAGTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTT10500.1733620178678453No Hit
GTCACTGCACAGGCTCTGTGACCTCTTATGTGGTGACTCAGCCACCCTCGATGTCAGTGGCCCCAGGACAGACGG10280.16972967082680473No Hit
CCCCTGCGCTGGTCGTCTATGATGATAGCGACCGGCCCTCAGGGATCCCT10110.16692285720418248No Hit
GGCTCTGTGACCTCTTATGTGGTGACTCAGCCACCCTCGATGTCAGTGGCCCCAGGACAGACGGCCCGGATTAC9910.16362072353050924No Hit
CCTCTGTCACTGCACAGGCTCTGTGACCTCTTATGTGGTGACTCAGCCAC9890.16329051016314192No Hit
GCCCTCAGGGATCCCTGAACGATTCTCCGGCTCCAACTCTGGGAACACGG9860.16279519011209093No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG9830.16229987006103994No Hit
GGGTGGCCGTGTTCCCAGAGTTGGAGCCGGAGAATCGTTCAGGGATCCCTGAGGGCCGGTCGCTATCATCATAGA9760.16114412327525432No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC9650.15932794975473402No Hit
GATTACCTGTGGGGGAAACAACATTGAAAGGAAAAGTGTGCAGTGGTACC9620.15883262970368303No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG9590.15833730965263204No Hit
CAGTAATAGTCGGCCTCATCCCCGGCTTCGACCCTGCTGATGGTCAGGGT9530.1573466695505301No Hit
GCGCAGGGGCCTGGCCTGGCTTCTGCTGGTACCACTGCACACTTTTCCTT9310.15371432250948952No Hit
GGCCAGGCCCCTGCGCTGGTCGTCTATGATGATAGCGACCGGCCCTCAGG8960.14793558858056133No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA8810.1454589883253064No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG8800.14529388164162274No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC8720.14397302817215343No Hit
CTCTTATACCACATACAACCCGTCCTTCCAAGGCCAGGTCACCATCTCAGCCGGCAAGTCCATCAACACCGCCTA8580.14166153460058217No Hit
GGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTGTCACTGCACAGGCTCTGTGACCTCTTATGTGGTGACTCAGC8560.14133132123321485No Hit
GTAATATATGGCGGTGTCCGAGGCCTTCAGGCTGCCCCACTGCAGGTAGG8540.14100110786584752No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA8510.14050578781479653No Hit
GTTCTCCTCCTCGGCCTCCTCTGTCACTGCACAGGCTCTGTGACCTCTTA8450.13951514771269458No Hit
GCACAGGCTCTGTGACCTCTTATGTGGTGACTCAGCCACCCTCGATGTCA8330.13753386750849061No Hit
ACCTAGGACGGTAACCTTGGTCCCAGCTCCAAAGACATAATGATCACTAC8320.13736876082480695No Hit
CTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTG8060.13307598704903173No Hit
AGGTTACCGTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAG7870.12993896005904215No Hit
GTAATAGTCGGCCTCATCCCCGGCTTCGACCCTGCTGATGGTCAGGGTGG7640.12614150633431792No Hit
CTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCC7580.12515086623221594No Hit
GGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTG7270.12003255903802243No Hit
GACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT7240.11953723898697143No Hit
GGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTGTCACTGCACAGGCTCTG7230.11937213230328776No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT7210.11904191893592043No Hit
GGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGC7150.11805127883381848No Hit
GTGGTAGTGATCATTATGTCTTTGGAGCTGGGACCAAGGTTACCGTCCTAGGTCAGCCCAAGGCCAACCCCA7050.11640021199698185No Hit
CCCTCGATGTCAGTGGCCCCAGGACAGACGGCCCGGATTACCTGTGGGGGAAACAACATTGAAAGGAAAAGTGTG7040.11623510531329818No Hit
CCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCA6850.11309807832330859No Hit
CCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGG6710.11078658475173733No Hit
CTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCCCG6710.11078658475173733No Hit
GGCCACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCC6600.10897041123121706No Hit
GACTATTACTGTCAGGTGTGGGATGGTGGTAGTGATCATTATGTCTTTGG6550.10814487781279875No Hit
GGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAG6480.10698913102701312No Hit
GGCCTGGCCTGGCTTCTGCTGGTACCACTGCACACTTTTCCTTTCAATGTTGTTTCCCCCACAGGTAATCCGGGC6460.10665891765964579No Hit
GGACAGACGGCCCGGATTACCTGTGGGGGAAACAACATTGAAAGGAAAAG6420.10599849092491112No Hit
ATATATTACTGTGCGAGACGGAGTGGAAACAACTATGGTTCTGGAAACTTCTTTGACTCCTGGGGCCAGGGAACC6390.10550317087386016No Hit
GAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG6390.10550317087386016No Hit
CCACCAAACCCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTAC6360.10500785082280917No Hit
GACCATCAGCAGGGTCGAAGCCGGGGATGAGGCCGACTATTACTGTCAGG6350.10484274413912549No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGG6340.10467763745544184No Hit
CACTACCACCATCCCACACCTGACAGTAATAGTCGGCCTCATCCCCGGCTTCGACCCTGCTGATGGTCAGGGTGG6290.10385210403702352No Hit
CAGGTAATCCGGGCCGTCTGTCCTGGGGCCACTGACATCGAGGGTGGCTGAGTCACCACATAAGAGGTCACAGAG6250.10319167730228887No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT6180.10203593051650323No Hit
TCTATGATGATAGCGACCGGCCCTCAGGGATCCCTGAACGATTCTCCGGCTCCAACTCTGGGAACACGGCCACC6180.10203593051650323No Hit
CCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAG6130.10121039709808492No Hit
GGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGGAAC6080.1003848636796666No Hit
CCACATAAGAGGTCACAGAGCCTGTGCAGTGACAGAGGAGGCCGAGGAGGAGAACGGTCCAGGCCATGGCTGAGG6060.10005465031229929No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)