FastQCFastQC Report
Wed 8 May 2024
EGAF00004689738

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689738
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences605669
Total Bases44.6 Mbp
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG29220.48244172972366095No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTC27770.45850126058952995No Hit
CTTATGTGGTGACTCAGCCACCCTCGATGTCAGTGGCCCCAGGACAGACG26050.43010291099594006No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT24520.4048415883923397No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA24460.4038509482902377No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCT22810.3766083454824335No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACA20880.3447427555314867No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC20160.332855074306263No Hit
GGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTGTCACTGCACAGGCTCTG20070.33136911415311004No Hit
GCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTGTCACTGCACAGG18540.3061077915495097No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC18200.3004941643042652No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC16670.27523284170066487No Hit
GCCTTGGGCTGACCTAGGACGGTAACCTTGGTCCCAGCTCCAAAGACATA16340.269784321139104No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA15370.2537689728217888No Hit
CCCCAGGACAGACGGCCCGGATTACCTGTGGGGGAAACAACATTGAAAGG15360.25360386613810515No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT15270.25211790598495215No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTAACCTTGGTC15130.24980641241338092No Hit
GACCTAGGACGGTAACCTTGGTCCCAGCTCCAAAGACATAATGATCACTA14620.24138597154551414No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT14430.23824894455552456No Hit
GCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGCCTC13980.23081914378975973No Hit
GTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATG13640.22520551654451526No Hit
GAGTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCA13350.22041742271768902No Hit
GCGCAGGGGCCTGGCCTGGCTTCTGCTGGTACCACTGCACACTTTTCCTT13190.21777571577875043No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA13160.21728039572769947No Hit
GTATCAACGCAGAGTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGG12910.21315272863560789No Hit
GGGTGGCCGTGTTCCCAGAGTTGGAGCCGGAGAATCGTTCAGGGATCCCT12880.2126574085845569No Hit
GGCCAGGCCCCTGCGCTGGTCGTCTATGATGATAGCGACCGGCCCTCAGG12660.20902506154351636No Hit
CTATTACTGTCAGGTGTGGGATGGTGGTAGTGATCATTATGTCTTTGGAG12650.2088599548598327No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG12520.20671356797194507No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC12380.2044020744003738No Hit
CCTCTGTCACTGCACAGGCTCTGTGACCTCTTATGTGGTGACTCAGCCAC12210.20159526077775156No Hit
CGGCCACCCTGACCATCAGCAGGGTCGAAGCCGGGGATGAGGCCGACTAT11710.19333992659356844No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCG11390.18805651271569124No Hit
GATTACCTGTGGGGGAAACAACATTGAAAGGAAAAGTGTGCAGTGGTACC11390.18805651271569124No Hit
GTCACTGCACAGGCTCTGTGACCTCTTATGTGGTGACTCAGCCACCCTCG11310.18673565924622193No Hit
GGCTCTGTGACCTCTTATGTGGTGACTCAGCCACCCTCGATGTCAGTGGC11210.18508459240938532No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT11180.18458927235833433No Hit
GTTCTCCTCCTCGGCCTCCTCTGTCACTGCACAGGCTCTGTGACCTCTTA10920.18029649858255914No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCT10800.17831521837835518No Hit
CCCCTGCGCTGGTCGTCTATGATGATAGCGACCGGCCCTCAGGGATCCCT10780.17798500501098785No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC10420.172041164398376No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGT10280.16972967082680473No Hit
GTAATATATGGCGGTGTCCGAGGCCTTCAGGCTGCCCCACTGCAGGTAGG10240.16906924409207008No Hit
GCCCTCAGGGATCCCTGAACGATTCTCCGGCTCCAACTCTGGGAACACGG10160.16774839062260077No Hit
GGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTG10160.16774839062260077No Hit
ACCTAGGACGGTAACCTTGGTCCCAGCTCCAAAGACATAATGATCACTAC9900.16345561684682558No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGT9730.1606488032242033No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTG9550.15767688291789742No Hit
CTCTTATACCACATACAACCCGTCCTTCCAAGGCCAGGTCACCATCTCAG9450.15602581608106078No Hit
GCACAGGCTCTGTGACCTCTTATGTGGTGACTCAGCCACCCTCGATGTCA9430.15569560271369345No Hit
CTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCC9290.1533841091421222No Hit
GTGGTAGTGATCATTATGTCTTTGGAGCTGGGACCAAGGTTACCGTCCTA9280.15321900245843853No Hit
CCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGG8950.14777048189687766No Hit
CAGTAATAGTCGGCCTCATCCCCGGCTTCGACCCTGCTGATGGTCAGGGT8920.14727516184582667No Hit
GGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCC8850.14611941506004106No Hit
CTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTG8770.14479856159057175No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCC8690.14347770812110247No Hit
GGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGC8680.14331260143741878No Hit
GTAATAGTCGGCCTCATCCCCGGCTTCGACCCTGCTGATGGTCAGGGTGG8390.1385245076105926No Hit
CTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCCCG8390.1385245076105926No Hit
AGGTTACCGTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTC8330.13753386750849061No Hit
ATATATTACTGTGCGAGACGGAGTGGAAACAACTATGGTTCTGGAAACTT8220.13571769398797032No Hit
GGCCTGGCCTGGCTTCTGCTGGTACCACTGCACACTTTTCCTTTCAATGT8130.13423173383481737No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG8120.1340666271511337No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGC8100.13373641378376638No Hit
CTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCA8030.13258066699798074No Hit
GTGGTACCAGCAGAAGCCAGGCCAGGCCCCTGCGCTGGTCGTCTATGATG8000.13208534694692975No Hit
GGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAG7940.1310947068448278No Hit
CCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCA7910.1305993867937768No Hit
GACTATTACTGTCAGGTGTGGGATGGTGGTAGTGATCATTATGTCTTTGG7680.12680193306905257No Hit
GACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT7630.12597639965063426No Hit
CCACCAAACCCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTAC7570.12498575954853228No Hit
ACCTGAGCCTGACGCCCGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC7490.123664906079063No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTG7350.12135341250749171No Hit
TCTATGATGATAGCGACCGGCCCTCAGGGATCCCTGAACGATTCTCCGGC7290.12036277240538974No Hit
CCCTCGATGTCAGTGGCCCCAGGACAGACGGCCCGGATTACCTGTGGGGG7130.11772106546645116No Hit
GTCCTGGGGCCACTGACATCGAGGGTGGCTGAGTCACCACATAAGAGGTC7020.11590489194593086No Hit
GGACAGACGGCCCGGATTACCTGTGGGGGAAACAACATTGAAAGGAAAAG6930.1144189317927779No Hit
CCTTGGTCCCAGCTCCAAAGACATAATGATCACTACCACCATCCCACACC6850.11309807832330859No Hit
GTGTGGGATGGTGGTAGTGATCATTATGTCTTTGGAGCTGGGACCAAGGT6710.11078658475173733No Hit
CCACATAAGAGGTCACAGAGCCTGTGCAGTGACAGAGGAGGCCGAGGAGG6690.11045637138437No Hit
GGTGTGGGATGGTGGTAGTGATCATTATGTCTTTGGAGCTGGGACCAAGG6610.10913551791490071No Hit
TTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACAC6550.10814487781279875No Hit
GGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTC6520.10764955776174776No Hit
GAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGT6370.10517295750649282No Hit
GTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA6340.10467763745544184No Hit
GGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGA6280.10368699735333986No Hit
GCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGA6270.10352189066965621No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT6250.10319167730228887No Hit
GACCATCAGCAGGGTCGAAGCCGGGGATGAGGCCGACTATTACTGTCAGG6230.10286146393492154No Hit
TTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGG6210.10253125056755423No Hit
GAGTTGGAGCCGGAGAATCGTTCAGGGATCCCTGAGGGCCGGTCGCTATC6160.10170571714913591No Hit
GGAGTGGAGACCACCAAACCCTCCAAACAGAGCAACAACAAGTACGCGGC6110.1008801837307176No Hit
CCCTGACCATCAGCAGGGTCGAAGCCGGGGATGAGGCCGACTATTACTGT6090.10054997036335028No Hit
CCTCTTATGTGGTGACTCAGCCACCCTCGATGTCAGTGGCCCCAGGACAG6060.10005465031229929No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)