FastQCFastQC Report
Wed 8 May 2024
EGAF00004689741

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689741
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences895437
Total Bases66.1 Mbp
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC40310.4501712571627038No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT39320.43911520296793627No Hit
GTATTATAATATTGCTGACAGTAATAAACTGCCACATCCTCAGCCTGCAG27940.3120264183856597No Hit
TTATAATACTCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAGTCAAAC25120.2805334155278373No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC25120.2805334155278373No Hit
ATTATAATACTCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAGTCAAA24730.2761780002389894No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG24070.2688072974424778No Hit
ATAATACTCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAGTCAAACGA23890.2667971057707019No Hit
ACTTAGGAGGCTGTCCTGGTTTCTGCTGGTACCAAGCTAAGTAGTTTTTA23880.2666854284556033No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC23260.2597614349194863No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC23130.2583096298232036No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG22860.25529434231553977No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA22060.2463601571076469No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG21040.23496907096758343No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT20920.23362894318639948No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT20590.22994359178814366No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTC19860.2217911477859414No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG19570.2185525056480802No Hit
CTCCTAAGTTGCTCATTCACTGGGCATCTACCCGGAAATCCGGGGTCCCT19490.21765908712729093No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAA18090.20202426301347837No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTC17730.1980038796699265No Hit
CCCTTACACTCTCCTGTGCAGCCTCTGGCTTCACTTTCAGTGACGCCTAT17560.1961053653132493No Hit
GTTTTGTACAACTCCAACAATAAAAACTACTTAGCTTGGTACCAGCAGAA17360.19387181901127606No Hit
GTAATAGACGGCTGTGTCCTCGGTCTTCAGGCTGTTCATTTGCAGATACA16320.1822573782410153No Hit
AACCAGGACAGCCTCCTAAGTTGCTCATTCACTGGGCATCTACCCGGAAA15950.17812531758236483No Hit
CACCATCACCAGCCTGCAGGCTGAGGATGTGGCAGTTTATTACTGTCAGC15790.17633848054078624No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT15770.17611512591058892No Hit
CTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCA15710.17544506201999693No Hit
GTTTATTACTGTCAGCAATATTATAATACTCCGTGGACGTTCGGCCAAGG15570.17388157960861567No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACT15560.17376990229351702No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG15150.16919113237447192No Hit
CTCCTGTGCAGCCTCTGGCTTCACTTTCAGTGACGCCTATATGAGCTGGG14980.16729261801779466No Hit
GAGTATTATAATATTGCTGACAGTAATAAACTGCCACATCCTCAGCCTGC14850.16584081292151207No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC14730.16450068514032812No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGACTTCCACCTTGGTCC14630.16338391198934152No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGG14550.16249049346855224No Hit
GTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCAC14280.15947520596088838No Hit
CCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGC14100.15746501428911247No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAG14060.15701830502871783No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG14030.15668327308342184No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT13870.15489643604184325No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAA13840.15456140409654728No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGC13100.14629728277924633No Hit
GTAATAAACTGCCACATCCTCAGCCTGCAGGCTGGTGATGGTGAGAGTGA13030.1455155415735557No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT12910.14417541379237178No Hit
GATTTCACTCTCACCATCACCAGCCTGCAGGCTGAGGATGTGGCAGTTTA12800.14294696332628648No Hit
GGACTGAACACAGAGGACTCACCATGGAATTTGGGCTGAGCTGGATTTTC12740.14227689943569455No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT12730.14216522212059587No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGC12210.13635800173546547No Hit
GTGCAGCCACAGTTCGTTTGACTTCCACCTTGGTCCCTTGGCCGAACGTC12200.1362463244203668No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCT12060.13468284200898556No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC11880.13267265033720965No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACT11760.13133252255602573No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG11690.1305507813503351No Hit
GTCCTGGTTTCTGCTGGTACCAAGCTAAGTAGTTTTTATTGTTGGAGTTG11680.13043910403523643No Hit
GTCAGGGACCCCGGATTTCCGGGTAGATGCCCAGTGAATGAGCAACTTAG11380.12708878458227657No Hit
GTCTTCAGGCTGTTCATTTGCAGATACAGCGTGTCCTTCGAATTATCTCT11240.12552530217089533No Hit
GGACCAAGGTGGAAGTCAAACGAACTGTGGCTGCACCATCTGTCTTCATC11210.12519027022559934No Hit
GTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAATGTTT11200.1250785929105007No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAG11060.12351511049911942No Hit
ATATTATAATACTCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAGTCA10940.12217498271793548No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT10910.1218399507726395No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA10900.12172827345754084No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGAC10840.12105820956694888No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCC10820.12083485493675156No Hit
ATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCACCAGCC10770.12027646836125824No Hit
GTACAAAACATTCTGGCTGGACTTGCAGTTGATGGTGGCCCTCTCGCCCA10700.11949472715556762No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC10690.11938304984046896No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC10340.11547434381201582No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC10060.11234737898925329No Hit
CTACGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGCTGTGTCTC10040.11212402435905597No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA9930.1108955738929707No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA9910.11067221926277337No Hit
GGATCTCTGGTGCCTACGGGGACATCGTGATGACCCAGTCTCCAGACTCC9900.11056054194767471No Hit
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT9860.11011383268728007No Hit
GGCTGAGGATGTGGCAGTTTATTACTGTCAGCAATATTATAATACTCCGT9830.10977880074198407No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT9820.10966712342688541No Hit
GATCAGGACTGAACACAGAGGACTCACCATGGAATTTGGGCTGAGCTGGA9710.10843867296080015No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTT9690.10821531833060283No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA9670.1079919637004055No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA9510.1062051266588269No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACT9480.10587009471353093No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG9480.10587009471353093No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG9410.1050883535078403No Hit
GTGTAAGGGACTCCCCAGGCTTTACCAAGCCTCCCCCAGACTCCACCAGG9370.10464164424744567No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGT9320.10408325767195235No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA9310.1039715803568537No Hit
TCCTCAGCCTGCAGGCTGGTGATGGTGAGAGTGAAATCTGTCCCAGACCC9290.10374822572665637No Hit
GCCTTGGGATTCCCAAGTGTTTTCATTCAGTGATCAGGACTGAACACAGA9220.10296648452096575No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA9150.1021847433152751No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG9130.10196138868507779No Hit
GTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGTGACCC9020.10073293821899251No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT9010.10062126090389384No Hit
CCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGTG8960.10006287432840055No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)