FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004689742

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689742
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences895437
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT33870.37825106623916593No Hit
GTATTATAATATTGCTGACAGTAATAAACTGCCACATCCTCAGCCTGCAGGCTGGTGATGGTGAGAGTGAAAT32230.3599359865629855No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC23790.2656803326197153No Hit
ATTATAATACTCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAGTCAAA23050.2574162113024143No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG22300.24904041267001475No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC22290.2489287353549161No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC21720.2425631283942924No Hit
TTATAATACTCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAGTCAAACGAACTGTGGCTGCACCATCTGTCTT20850.23284720198070885No Hit
ACTTAGGAGGCTGTCCTGGTTTCTGCTGGTACCAAGCTAAGTAGTTTTTATTGTTGGAGTTGTACAAAACATT20520.22916185058245303No Hit
ATAATACTCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAGTCAAACGA19230.21475547693472571No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG19160.2139737357290351No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG18620.2079431607137074No Hit
GTAATAAACTGCCACATCCTCAGCCTGCAGGCTGGTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCA17770.1984505889303212No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT17450.19487691484716402No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT17100.19096820881871088No Hit
GAGTATTATAATATTGCTGACAGTAATAAACTGCCACATCCTCAGCCTGCAGGCTGGTGATGGTGAGAGTGAAAT16890.18862298520163898No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG16090.17968879999374607No Hit
CTCCTAAGTTGCTCATTCACTGGGCATCTACCCGGAAATCCGGGGTCCCT15500.17309983840292506No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG15330.17120132404624783No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA15250.17030790552545852No Hit
GTTTATTACTGTCAGCAATATTATAATACTCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAGTCAAACGAA15000.16751597264799198No Hit
CCCTTACACTCTCCTGTGCAGCCTCTGGCTTCACTTTCAGTGACGCCTAT14720.16438900782522947No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA14230.15891681938539506No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT13950.15578985456263253No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13910.1553431453022379No Hit
CTCCTGTGCAGCCTCTGGCTTCACTTTCAGTGACGCCTATATGAGCTGGGTCCGCCAGGCTCCAGGGAAGGGG13720.15322127631536334No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA13470.1504293434378968No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG13180.14719070130003561No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG12810.14305864064138515No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT12780.1427236086960892No Hit
CACCATCACCAGCCTGCAGGCTGAGGATGTGGCAGTTTATTACTGTCAGCAATATTATAATACTCCGTGGACGTT12760.14250025406589184No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT12670.1414951582300039No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC12330.13769812951664942No Hit
GTTTTGTACAACTCCAACAATAAAAACTACTTAGCTTGGTACCAGCAGAA12170.13591129247507083No Hit
GTAATAGACGGCTGTGTCCTCGGTCTTCAGGCTGTTCATTTGCAGATACA12060.13468284200898556No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAATGTTTTGTA11990.13390110080329493No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG11910.13300768228250562No Hit
TCCTCAGCCTGCAGGCTGGTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACC11660.1302157494050391No Hit
CTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAATGTTTTGTACAACTCCAA11650.13010407208994046No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC11600.12954568551444715No Hit
GGACTGAACACAGAGGACTCACCATGGAATTTGGGCTGAGCTGGATTTTC11360.12686542995207925No Hit
AACCAGGACAGCCTCCTAAGTTGCTCATTCACTGGGCATCTACCCGGAAA11250.12563697948599398No Hit
GTCTTCAGGCTGTTCATTTGCAGATACAGCGTGTCCTTCGAATTATCTCT11230.12541362485579666No Hit
GTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCAC11140.12440852901990872No Hit
GATTTCACTCTCACCATCACCAGCCTGCAGGCTGAGGATGTGGCAGTTTATTACTGTCAGCAATATTATAATACT11140.12440852901990872No Hit
ATATTATAATACTCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAGTCAAACGAACTGTGGCTGCACCAT10900.12172827345754084No Hit
GTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACCCCGGATTTCCGGGT10720.11971808178576494No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG10650.11893634058007432No Hit
GTGCAGCCACAGTTCGTTTGACTTCCACCTTGGTCCCTTGGCCGAACGTC10500.1172611808535944No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT10490.11714950353849574No Hit
GAGTGAAATCTGTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACCCCG10490.11714950353849574No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC10480.11703782622339708No Hit
GGCTGAGGATGTGGCAGTTTATTACTGTCAGCAATATTATAATACTCCGT10330.11536266649691716No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGACTTCCACCTTGGTCCCTTGGCCGAACGTCCACGGAGTATT10140.11324079751004257No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG9780.10922041416649077No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT9680.10810364101550417No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT9670.1079919637004055No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC9630.10754525444001085No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG9470.10575841739843227No Hit
CCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGC9440.10542338545313629No Hit
GTCCTGGTTTCTGCTGGTACCAAGCTAAGTAGTTTTTATTGTTGGAGTTG9440.10542338545313629No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT9390.10486499887764299No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA9360.10452996693234701No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA9320.10408325767195235No Hit
GTCAGGGACCCCGGATTTCCGGGTAGATGCCCAGTGAATGAGCAACTTAGGAGGCTGTCCTGGTTTCTGCTGGTA9020.10073293821899251No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)