FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689743

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689743
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences371608
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTATTGTCTTCAGACTGAGACTTTACCTCTAACTTTCGGCGGGGGGA35150.9458892165938302No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT15020.4041893608318443No Hit
CAATAATACCTTGCAGCGTCTTCCGGTTCTAGGCTATTGATGGTGAGGGTGAATTCTGTCCCAGATCCGCCGCCA14740.3966545391918366No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC13160.35413661708036426No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGT9590.2580676411702654No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC9140.24595810639168156No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG8950.24084519170739058No Hit
GTTCTGACTCAGTCTCCAGAGTTTCAGTCTGTGATTCCGAAGGAGACAGT7800.2098986028287873No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG7530.202632881961637No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG7470.20101827732449248No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC7190.19348345568448472No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT6880.18514133172590472No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT6720.180835719360186No Hit
GAATTCACCCTCACCATCAATAGCCTAGAACCGGAAGACGCTGCAAGGTATTATTGTCTTCAGACTGAGACTTTA6380.17168629308303374No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG6370.17141719231017633No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC6160.16576607608017052No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG6110.1644205722158834No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA6060.1630750683515963No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT5920.15930765753159243No Hit
GAACTTACACTGGTATCAGCAGAAGTCAGATCAGTCTCCAAAGCTCCTCA5710.15365654130158662No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT5630.15150373511872725No Hit
CTCCAGGGGTGACGTTGTTCTGACTCAGTCTCCAGAGTTTCAGTCTGTGATTCCGAAGGAGACAGTCACCATCAC5560.14962002970872532No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATGTCCACTTCGGTCCCCCCGCCGAAAGTTAGAGGTAAAGT5410.14558351811586404No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA4940.13293578179156532No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT4700.12647736324298725No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC4610.12405545628727047No Hit
GGGTTCCAGCCTCCAGGGGTGACGTTGTTCTGACTCAGTCTCCAGAGTTT4550.12244085165012594No Hit
GTATCAACGCAGAGTACGGGATGAGCAAAACTGACAAGTCAAGGCAGGAA4520.1216335493315537No Hit
GGTATCAGCAGAAGTCAGATCAGTCTCCAAAGCTCCTCATCAAGTATGTT4480.12055714624012401No Hit
GTCCTCGTCAGGGTCGAGGTTCAGTGGCGGCGGATCTGGGACAGAATTCA4390.11813523928440722No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG4370.1175970377386924No Hit
GACGAGGACTGAGAAACATACTTGATGAGGAGCTTTGGAGACTGATCTGACTTCTGCTGATACCAGTGTAAGTT4270.11490603001011819No Hit
GTGTAAGTTCTTCCCAATGTTTTCGCTGGCCCGGCAGGTGATGGTGACTGTCTCCTTCGGAATCACAGACTGAAA4210.11329142537297367No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC4160.11194592150868658No Hit
GAGCAAAACTGACAAGTCAAGGCAGGAAGATGTTGGCATCACAACTCATTGGGTTTCTGCTGCTCTGGGTTCCAG4120.1108695184172569No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT4050.10898581300725495No Hit
GGACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGATCTTC4000.10764030914296786No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG3990.10737120837011044No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG3970.1068330068243956No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG3850.10360379755010657No Hit
ACGTTGTTCTGACTCAGTCTCCAGAGTTTCAGTCTGTGATTCCGAAGGAG3840.10333469677724913No Hit
AACTTACACTGGTATCAGCAGAAGTCAGATCAGTCTCCAAAGCTCCTCAT3840.10333469677724913No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA3830.10306559600439173No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC3800.10225829368581947No Hit
GTGCAGCCACAGTTCGTTTGATGTCCACTTCGGTCCCCCCGCCGAAAGTT3780.10172009214010462No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTAACG150.002505774667.92332
GCGTATG252.573487E-455.1625240
GTCGTAT252.6013967E-455.04222539
AACTGTG9800.054.330570
GTATCGG200.007374827851.71486340
TGTATCG200.007438770551.60208539
TAGGCGG200.007438770551.60208539
CGCGTAT200.007438770551.60208539
TCGCGTA200.007491026551.51081538
TAATCGC200.007616739751.2944735
CGTATGC2000.046.63255341
TCGTATG1800.045.96876540
ATGCCGT2050.044.0028744
TATGCCG2050.043.9424743
GCTAATC1700.042.16823633
ATCTCCG2350.041.91012210
CCCACGA2350.041.91012219
CACGAGA2500.040.75946821
GTATGCC2300.040.6390342
TCTCCGA2450.040.1995111
Produced by FastQC (version 0.11.5)