FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004689746

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689746
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1647141
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG61720.37470987608225403No Hit
GTAATATAGGGCCGTGTCCTCGGCTCTCAGGCTGCTCATTTGCAGAGACA54460.33063350374983075No Hit
TCTCTGGACTGCAGACTGAGGACGAGTCTGACTACTACTGTCAGTCTTAT33410.20283630848846576No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC32830.1993150556024044No Hit
CAGTAATATAGGGCCGTGTCCTCGGCTCTCAGGCTGCTCATTTGCAGAGA31620.19196899354700053No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG31410.19069405715721968No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCAC31310.1900869445906574No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG30660.1861407129080024No Hit
GCCCCACTCTGTGTCGGAGTCTCCGGGGAAGACGGTAACCATCTCCTGCACCCGCAGCAGTGGCAGCATTGCCAG28390.1723592576470381No Hit
GTGTCGGAGTCTCCGGGGAAGACGGTAACCATCTCCTGCACCCGCAGCAGTGGCAGCATTGCCAGCAACTATGTG28250.1715093000538509No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT26350.1599741612891671No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT26100.15845637987276134No Hit
GTCTTATGATAGCGGCAATGTGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCC24410.1481961774978584No Hit
ATCATAAGACTGACAGTAGTAGTCAGACTCGTCCTCAGTCTGCAGTCCAG24130.14649626231148397No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT23330.14163936177898553No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA23140.14048584790251714No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG22640.13745028506970564No Hit
GTCCAGAGATGGTGAGGGAGGCAGAGTTGGAGGAGCTGTCGATGGAGCCA22240.13502183480345642No Hit
ATATTACTGTGCGAAGGCGGTCCATAGCAGTGGCTTTATCTTTGACTACTGGGGCCAGGGAACCCTGGTCACCGT21940.1332004971037695No Hit
CTATATTACTGTGCGAAGGCGGTCCATAGCAGTGGCTTTATCTTTGACTA21840.13259338453720718No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC21190.12864715285455222No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTTAGCACCTATGCCATGAGCTGGGTCCGCCAGGCTCCAGGGAAGGGG20640.12530803373845956No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG20610.12512589996849086No Hit
GACTACTACTGTCAGTCTTATGATAGCGGCAATGTGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAG20380.12372954106539757No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT19120.11607992272671254No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG18540.11255866984065117No Hit
GCTATGGACCGCCTTCGCACAGTAATATAGGGCCGTGTCCTCGGCTCTCA18350.1114051559641828No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC18300.11110159968090164No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG18290.11104088842424541No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG17830.10824817061805882No Hit
CTGCACAGGTTCTTGGGCCAATTTTATGCTGACTCAGCCCCACTCTGTGTCGGAGTCTCCGGGGAAGACGGTAAC17710.10751963553818403No Hit
CCTTTAGCACCTATGCCATGAGCTGGGTCCGCCAGGCTCCAGGGAAGGGG17690.1073982130248716No Hit
GTAGTAGTCAGACTCGTCCTCAGTCTGCAGTCCAGAGATGGTGAGGGAGG17550.10654825543168435No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC17200.10442336144871628No Hit
ATATAGGGCCGTGTCCTCGGCTCTCAGGCTGCTCATTTGCAGAGACAGCG16790.10193419992581085No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)