FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004689749

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689749
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1363453
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATATACAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGAAACA86580.635005387057713No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT41180.30202727926815226No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC37040.2716631963111306No Hit
CTACATGACCTGGATCCGCCAGGCTCCAGGGAAGGGGCTGGAGTGGGTTTCATACATTACTGGAAGTAGTAGTTA36590.2683627525114544No Hit
GTATATTACTGTGCGAGACTCAAGAATAAGGTGACTACGTCGCCTTTTGA33590.24635979384694592No Hit
GAGTTACAGTACCCCTCAGACGTTCGGCCAAGGGACCAAGGTCGAAATCAAACGAACTGTGGCTGCACCAT33360.24467290034933367No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG29060.21313532626353826No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG28590.20968819607276526No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC27570.20220719012683236No Hit
GATCTATGCTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTTCACTGGCAGTGGTTCTGGGACAGATTTCA27260.19993355106483318No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGCATTAACAGCTTTTTAAATTGGTATCAGCAGAAA24180.17734384683593787No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA23220.17030290006329518No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC22750.1668557698725222No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG22670.1662690243081353No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTCAGTGACCACTACATGACCTGGATCCGCCAGGCTCCAGGGAAGGGG22580.16560893554820005No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG22290.16348198287729757No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA22100.1620884621618787No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGCATTAACAG21640.15871467516665408No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT20930.1535073082827204No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG20790.15248050354504336No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT20560.15079361004743103No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG20510.15042689406968923No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT20230.1483732845943351No Hit
GCATAGATCAGGAGCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAATT20130.14763985263885152No Hit
CAGTAATATACAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGAAA19730.14470612481691705No Hit
GTACTGTAACTCTGCTGACAGTAATAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAAT19410.1423591425593695No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC19300.14155236740833751No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG19280.14140568101724077No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT19210.14089227864840226No Hit
AGCCTGGAGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTC19190.1407455922573055No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCGACCTTGGTCCCTTGGCCGAACGTCTGAGGGGTA18880.13847195319530634No Hit
GTTACAGTACCCCTCAGACGTTCGGCCAAGGGACCAAGGTCGAAATCAAA18740.13744514845762928No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC18730.1373718052620809No Hit
CTGTAACTCTGCTGACAGTAATAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAATCTGTC18620.13656503011104892No Hit
GCACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGGTTTTC18380.13480479341788826No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT18290.134144704657953No Hit
GATTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTATTACTGTCAGCAGAGTTACAGTACC18070.13253115435588905No Hit
GTATATTACTGTGCGAGACTCAAGAATAAGGTGACTACGTCGCCTTTTGACTACTGGGGCCAGGGAACCCTGGT17450.12798387623189064No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT16940.1242433732589242No Hit
GATCAGGAGCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAATTTAAAA16130.11830257441950694No Hit
GAGTGAAATCTGTCCCAGAACCACTGCCAGTGAACCTTGATGGGACCCCA15970.11712908329073315No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG15760.11558887618421758No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG15740.11544218979312085No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA15250.11184837321125114No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT14970.10979476373589703No Hit
GTATCAACGCAGAGTACGGGAGCTCTGGGAGAAGAGCCCCAGCCCCAGAATTCCCAGGAGTTTCCATTCGGTGAT14900.10928136136705849No Hit
CCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACCAGGGTTCCCTGG14860.10898798858486505No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT14810.10862127260712325No Hit
GTGCAGCCACAGTTCGTTTGATTTCGACCTTGGTCCCTTGGCCGAACGTC14650.10744778147834945No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14650.10744778147834945No Hit
GAGTTTGGGCTGAGCTGGGTTTTCCTTCTTGCTATTATAAAAGGTGTCCAGTGTCAGGTGCAGCTGGTGGAGT14460.10605426076293059No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT14330.1051007992208019No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG14270.10466074004751172No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCC14210.10422068087422155No Hit
CTTATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGT13810.10128695305228709No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)