FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004689750

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689750
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1363453
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATATACAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGAAACA82660.6062548544027554No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG61330.4498138182981005No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT40640.2980667467085407No Hit
GTATATTACTGTGCGAGACTCAAGAATAAGGTGACTACGTCGCCTTTTGA34300.25156716073087965No Hit
CTACATGACCTGGATCCGCCAGGCTCCAGGGAAGGGGCTGGAGTGGGTTTCATACATTACTGGAAGTAGTAGTTA33210.2435727524161082No Hit
GAGTTACAGTACCCCTCAGACGTTCGGCCAAGGGACCAAGGTCGAAATCAAACGAACTGTGGCTGCACCAT32350.23726523759894916No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC30130.2209830481872129No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG27220.19964017828263975No Hit
GATCTATGCTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTTCACTGGCAGTGGTTCTGGGACAGATTTCA26420.19377272263877082No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC26300.1928926042921905No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC25600.18775858060380518No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG24460.17939745631129198No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGCATTAACAGCTTTTTAAATTGGTATCAGCAGAAA23130.16964281130335992No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTCAGTGACCACTACATGACCTGGATCCGCCAGGCTCCAGGGAAGGGG21800.15988816629542787No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGCATTAACAG21110.15482748580259093No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT20730.15204044437175318No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG20660.15152704200291467No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA19900.14595295914123918No Hit
GTACTGTAACTCTGCTGACAGTAATAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAAT19820.1453662135768523No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT19730.14470612481691705No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA19710.14455943842582034No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG19450.14265251534156292No Hit
GTATATTACTGTGCGAGACTCAAGAATAAGGTGACTACGTCGCCTTTTGACTACTGGGGCCAGGGAACCCTGGT19170.1405989058662088No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG18820.13803189402201615No Hit
CTGTAACTCTGCTGACAGTAATAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAATCTGTC18780.13773852123982272No Hit
GCATAGATCAGGAGCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAATT18780.13773852123982272No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT18350.13458476383124318No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT18340.13451142063569482No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT18190.13341127270246939No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC17900.13128432003156693No Hit
CAGTAATATACAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGAAA17880.13113763364047018No Hit
GATTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTATTACTGTCAGCAGAGTTACAGTACC17520.12849727860072918No Hit
GTTACAGTACCCCTCAGACGTTCGGCCAAGGGACCAAGGTCGAAATCAAA17400.12761716025414885No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC17050.12505014840995618No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCGACCTTGGTCCCTTGGCCGAACGTCTGAGGGGTA16990.12461008923666601No Hit
GCACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGGTTTTC16660.12218976378357009No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG16620.12189639100137666No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA16610.12182304780582828No Hit
AGCCTGGAGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTC16260.11925603596163566No Hit
GAGTGAAATCTGTCCCAGAACCACTGCCAGTGAACCTTGATGGGACCCCA15820.11602893535750773No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC15720.11529550340202413No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT15580.11426869866434707No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT15360.11265514836228312No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG15090.11067488208247736No Hit
GTAATATACAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGAAACAGTGAGTTCTTGGCGTTGTCTCTGGA15070.11052819569138063No Hit
GATCAGGAGCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAATTTAAAA14880.10913467497596177No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT14840.10884130219376834No Hit
CCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACCAGGGTTCCCTGG14530.10656766313176913No Hit
GTGGGGTCCCATCAAGGTTCACTGGCAGTGGTTCTGGGACAGATTTCACT14440.10590757437183387No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC14090.1033405625276412No Hit
GTTCAAGGACCACCTGACCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG14070.10319387613654449No Hit
GTGCAGCCACAGTTCGTTTGATTTCGACCTTGGTCCCTTGGCCGAACGTC14060.10312053294099614No Hit
GTATCAACGCAGAGTACGGGAGCTCTGGGAGAAGAGCCCCAGCCCCAGAATTCCCAGGAGTTTCCATTCGGTGAT13990.1026071305721576No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)