FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004689755

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689755
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1050407
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATAATAGTTCTTTCCTGTACATTTTTGGCCAGGGGACCAAGCTTGAGATCAGACGAACTGTGGCTGCACCAT47510.4523008700437069No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT47320.4504920473683058No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC42180.40155863393903507No Hit
GAGTATTAGTAGATGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAACCTCCTGATGTGCAAGGCGT41690.3968937754603692No Hit
GTATTAGTAGATGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCT36800.35034039186715243No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGT33710.32092322309352467No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC33290.31692477296895394No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG31780.30254939275918763No Hit
CTATTATAGTGTTGGCAGTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTC28740.2736082299527707No Hit
GAATTCACTCTCACCATCAGCAGCCTGCAGCCTGATGATTTTGCAACTTA27700.2637073058347859No Hit
CACTATAATAGTTCTTTCCTGTACATTTTTGGCCAGGGGACCAAGCTTGA27460.2614224771921741No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG26110.24857031607748234No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC26020.2477135053365029No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG25020.2381933859922868No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA23720.2258172308448059No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT23680.22543642607103723No Hit
CCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCG23620.22486521891038427No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGAGTATTAGTAGATGGTTGGCCTGGTATCAGCAGAAA23000.21896274491697026No Hit
ATTATAGTGTTGGCAGTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGC22420.21344107569732496No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC22110.21048983870061796No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGA21480.20449216351376182No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT21350.2032545479990137No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT20740.19744727519904187No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTCTGATCTCAAGCTTGGTCC20390.19411523342856626No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT20370.19392483104168193No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG19590.18649913795319337No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG19150.1823102854417383No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG18560.1766934150286508No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAGTGTGACAT16450.15660596321235482No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16070.15298831786155273No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT16060.15289311666811053No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT15970.1520363059271311No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA15820.1506082880254987No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC15720.14965627609107707No Hit
ATAATAGTTCTTTCCTGTACATTTTTGGCCAGGGGACCAAGCTTGAGATCAGACGAACTGTGGCTGCACCAT15440.14699064267469655No Hit
CTACTAATACTCTGACTGGCCCGGCAAGTGATGGTGACTCTGTCTCCTACAGATGCAGACAGGGTGGAAGGAGA15360.1462290331271593No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT15140.14413460687143176No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAGTGTGACATCCAGATGACCCAGTCTCCTTCCACC14870.1415641746484934No Hit
GGTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGCCAACCATCTACTA14660.139564949586208No Hit
GTGTGACATCCAGATGACCCAGTCTCCTTCCACCCTGTCTGCATCTGTAG14450.13756572452392263No Hit
CATCAGGAGGTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGCCAACC14330.13642331020261672No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC13930.13261526246493027No Hit
GCCCCTAACCTCCTGATGTGCAAGGCGTCTATTTTACAAAGTGGGGTCCCGTCAAGGTTCAGCGGCAGTGGAT13870.1320440553042773No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG13760.13099684217641352No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG13470.12823600756659087No Hit
CTAATACTCTGACTGGCCCGGCAAGTGATGGTGACTCTGTCTCCTACAGA13440.12795040398626437No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG13380.12737919682561138No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC13250.1261415813108633No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG12870.1225239359600612No Hit
GGTATCAGCAGAAACCAGGGAAAGCCCCTAACCTCCTGATGTGCAAGGCGTCTATTTTACAAAGTGGGGTCCCGT12820.12204792999285038No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA12680.12071511328466014No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA12500.11900149180270124No Hit
CCCCTAACCTCCTGATGTGCAAGGCGTCTATTTTACAAAGTGGGGTCCCG12430.1183350834486061No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA12290.11700226674041586No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG12220.11633585838632073No Hit
ACTTATTACTGCCAACACTATAATAGTTCTTTCCTGTACATTTTTGGCCA12140.11557424883878345No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG12010.11433663332403535No Hit
GTCTCCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTT11990.11414623093715104No Hit
ATTTTACAAAGTGGGGTCCCGTCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACTCTCACCATCAGCAGC11940.11367022496994023No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT11880.11309901780928726No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT11720.11157579871421267No Hit
GTGCAGCCACAGTTCGTCTGATCTCAAGCTTGGTCCCCTGGCCAAAAATG11610.11052858558634891No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC11450.10900536649127432No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC11230.10691094023554679No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA11220.10681573904210463No Hit
GTGGGGTCCCGTCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACT11210.10672053784866248No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC11160.10624453188145166No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA11040.10510211756014573No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC11030.10500691636670358No Hit
CACCATCAGCAGCCTGCAGCCTGATGATTTTGCAACTTATTACTGCCAAC10750.10234128295032306No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT10750.10234128295032306No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG10710.10196047817655442No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)