FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689757

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689757
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1020431
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC57200.5605474549479582No Hit
GTCTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTCGGTGTCAGTGTCCCCAGGACAAACGGCCAGGATCAC54290.532030093166515No Hit
GCCTTGGGCTGACCCAGGACGGTCAGCTTGGTCCCACCGCCGAAAACCCC52700.5164484418838706No Hit
GTAATAGACAGCCGTGTCCCCGTCTGTCAGGCCGTTCATTTGCAGATACAGTGAGTTCTTGGCATTGTCCCTGGA39870.3907172557478164No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT33430.3276066681627665No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG31460.308301100221377No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAGGACGGTCAGCTTGGTCCCAC26580.26047817049854427No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT26310.257832229714699No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT23870.2339207648532826No Hit
GTCCTGGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC23560.23088283284220099No Hit
CCTATGAGCTGACACAGCCACCCTCGGTGTCAGTGTCCCCAGGACAAACGGCCAGGATCACCTGCTCTGGAGATG22950.22490496662684686No Hit
GTGCAGGGAGAAGGGCCTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC22290.2184371113774474No Hit
GAGAAGGGCCTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT21500.21069528463952977No Hit
CTACTACTGTTATTCAACAGACTTCACTAATACTCGTGGGGTTTTCGGCG21470.2104012912191025No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT20820.20403143377651206No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG20450.200405514924576No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG20250.19844555878839434No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG20150.19746558072030349No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC19580.1918797057321857No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA19470.1908017298572858No Hit
GTGTCAGTGTCCCCAGGACAAACGGCCAGGATCACCTGCTCTGGAGATGC19020.18639182855087702No Hit
GGCCAGGCCCCTGCTATGGTCATCTTTGACGACAAGGAGCGACCCTCCGG18970.18590183951683162No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC18520.18149193821042287No Hit
GTATTAGTGAAGTCTGTTGAATAACAGTAGTAGTCACCTTCATCCTCCAC17860.17502408296102334No Hit
CTTTCTGCACAGTCTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTCGGTGTCAGTGTCCCCAGGACAAACGG17490.17139816410908723No Hit
GTCCCTGACCTGGAGCCGGAGATTCTCTCAGGGATCCCGGAGGGTCGCTC17450.1710061728818509No Hit
CAGTAGTAGTCACCTTCATCCTCCACTTGGGCCCCACTGATAGTCAAGGT17310.16963420358652373No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA16830.1649303088596877No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG16790.1645383176324514No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA16730.16395033079159688No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT16430.16101039658732436No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCCTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG16310.15983442290561536No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA16090.15767847115581554No Hit
ACCCAGGACGGTCAGCTTGGTCCCACCGCCGAAAACCCCACGAGTATTAG15490.1517986027472705No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC15380.1507206268723706No Hit
CACTAATACTCGTGGGGTTTTCGGCGGTGGGACCAAGCTGACCGTCCTGGGTCAGCCCAAGGCTGCCCCCTCGGT15360.15052463125875243No Hit
CCCCAGGACAAACGGCCAGGATCACCTGCTCTGGAGATGCATTGCCAATA15170.14866267292937982No Hit
GGTCAGGGACAATGGCCACCTTGACTATCAGTGGGGCCCAAGTGGAGGATGAAGGTGACTACTACTGTTATTCAA15170.14866267292937982No Hit
GACCCAGGACGGTCAGCTTGGTCCCACCGCCGAAAACCCCACGAGTATTA15060.1475846970544799No Hit
ACCATATATTACGCGGACTCTGTGAAGGGCCGATTCACCGTGTCCAGGGACAATGCCAAGAACTCACTGTATCTG14960.1466047189863891No Hit
GGATGAAGGTGACTACTACTGTTATTCAACAGACTTCACTAATACTCGTGGGGTTTTCGGCGGTGGGACCAAG14630.14337079136168934No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC14160.1387648944416624No Hit
GCTCTGGAGATGCATTGCCAATAAGATATGTTTATTGGTACCATCAGAAG14050.13768691856676246No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCCTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG13970.1369029361122898No Hit
CTGCTATGGTCATCTTTGACGACAAGGAGCGACCCTCCGGGATCCCTGAGAGAATCTCCGGCTCCAGGTCAGGGA13610.13337501506716282No Hit
GTCTATTACTGTGCGAGCGGCTTCATGGACGTCTGGGGCCAAGGGACCACGGTCACCGTCTCCTCTGCATCCCCG13340.13072907428331754No Hit
CTCTTGGTACAGCCTGGGGAGTCCCTGAGACTCTCTTGTGCAGCCTCTGG13150.12886711595394496No Hit
GACTATCAGTGGGGCCCAAGTGGAGGATGAAGGTGACTACTACTGTTATT12940.12680916201095419No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG12860.12602517955648151No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG12850.12592718174967243No Hit
CCCCTCTCCTGCTCCCCCTCCTCACTTTCTGCACAGTCTCTGAGGCCTCC12770.12514319929519976No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAGGACGGTCAGC12760.12504520148839068No Hit
CTGATAGTCAAGGTGGCCATTGTCCCTGACCTGGAGCCGGAGATTCTCTC12610.12357523438625445No Hit
GTCCAGGGACAATGCCAAGAACTCACTGTATCTGCAAATGAACGGCCTGACAGACGGGGACACGGCTGTCTATTA12590.12337923877263628No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC12480.12230126289773635No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA12320.12073329798879101No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA12250.12004731334112743No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC12110.11867534404580025No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT12030.11789136159132758No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG11830.11593140545514591No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT11790.11553941422790957No Hit
GGCCTGGACCCCTCTCCTGCTCCCCCTCCTCACTTTCTGCACAGTCTCTGAGGCCTCCTATGAGCTGACACAGC11790.11553941422790957No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT11720.11485342958024598No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAGGACGGTCAGCTTGGTC11450.11220748879640073No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT11400.11171749976235533No Hit
CCTTCATCCTCCACTTGGGCCCCACTGATAGTCAAGGTGGCCATTGTCCC11390.11161950195554625No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG11320.11093351730788266No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA11110.1088755633648919No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT10870.10652361600147389No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC10750.10534764231976489No Hit
CCCCTGCTATGGTCATCTTTGACGACAAGGAGCGACCCTCCGGGATCCCT10750.10534764231976489No Hit
CGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC10720.10505364889933763No Hit
GGATCACCTGCTCTGGAGATGCATTGCCAATAAGATATGTTTATTGGTACCATCAGAAGTCAGGCCAGGCCCCTG10650.10436766425167404No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG10630.10417166863805588No Hit
CATCAGAAGTCAGGCCAGGCCCCTGCTATGGTCATCTTTGACGACAAGGA10510.10299569495634688No Hit
ATATATGGTAGTTCCAGTGGAATTAATGAATGAAATCCACTCCAGCCCCTTCCCTGGAGCCTGGCGGACCCAGTT10480.10270170153591962No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGC10470.10260370372911054No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC10470.10260370372911054No Hit
TAGCAGGGGCCTGGCCTGACTTCTGATGGTACCAATAAACATATCTTATT10360.10152572785421062No Hit
CCATAGCAGGGGCCTGGCCTGACTTCTGATGGTACCAATAAACATATCTT10360.10152572785421062No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG4500.066.93272470
ATCGTAT353.3630022E-758.94693839
TTATGCG200.00657971253.2352255
GTATCGC200.00779871550.99550229
CGTATGC6000.047.12969641
TCGTATG6150.046.4829940
GGTATCA5850.046.3776551
GATATCG306.5514026E-445.65244336
CGAGACT6400.045.56477723
ACGAGAC6400.045.5580822
ATGCCGT6300.045.1397344
GACTAGG6550.045.083226
ATCTCGT5250.045.040437
TATACAC6400.044.4855043
GTATGCC6450.044.4715742
TCTCGTA5500.044.31612838
CTTATAC6450.044.1666261
GCCGTCT6500.043.9159546
TAGGCAT6650.043.45481529
GATCTCG5550.043.18474236
Produced by FastQC (version 0.11.5)