FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689758

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689758
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1020431
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTCGGTGTCAGTGTCCCCAGGACAAACGGCCAGGATCAC61680.6044504723984278No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG60850.5963166544332738No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC54460.5336960558822694No Hit
GTAATAGACAGCCGTGTCCCCGTCTGTCAGGCCGTTCATTTGCAGATACAGTGAGTTCTTGGCATTGTCCCTGGA36280.35553604310335535No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG33400.32731267474233927No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT30530.2991873041881323No Hit
GCCTTGGGCTGACCCAGGACGGTCAGCTTGGTCCCACCGCCGAAAACCCC29650.29056349718893293No Hit
CCTATGAGCTGACACAGCCACCCTCGGTGTCAGTGTCCCCAGGACAAACGGCCAGGATCACCTGCTCTGGAGATG23320.22853088547878297No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT22890.22431697978599235No Hit
CTACTACTGTTATTCAACAGACTTCACTAATACTCGTGGGGTTTTCGGCG22050.21608516401402939No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT21590.21157726490081152No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAGGACGGTCAGCTTGGTCCCAC21550.21118527367357517No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG21320.20893132411696624No Hit
GTGTCAGTGTCCCCAGGACAAACGGCCAGGATCACCTGCTCTGGAGATGC21100.20677537236716642No Hit
GTGCAGGGAGAAGGGCCTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC20620.2020714776403304No Hit
GGCCAGGCCCCTGCTATGGTCATCTTTGACGACAAGGAGCGACCCTCCGG20470.20060151053819414No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCCTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG20030.1962896070385945No Hit
GTCCTGGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC19610.19217369915261298No Hit
GAGAAGGGCCTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT19410.1902137430164313No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG19210.18825378688024963No Hit
CTTTCTGCACAGTCTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTCGGTGTCAGTGTCCCCAGGACAAACGG19170.18786179565301328No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC19080.18697981539173153No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA18650.18276590969894094No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC18200.17835600839253218No Hit
GTATTAGTGAAGTCTGTTGAATAACAGTAGTAGTCACCTTCATCCTCCAC17350.17002619481376008No Hit
GTCCCTGACCTGGAGCCGGAGATTCTCTCAGGGATCCCGGAGGGTCGCTC17340.169928197006951No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA17100.167576249643533No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG16990.16649827376863305No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG16620.16287235491669697No Hit
GCTCTGGAGATGCATTGCCAATAAGATATGTTTATTGGTACCATCAGAAG16340.1601284163260426No Hit
CAGTAGTAGTCACCTTCATCCTCCACTTGGGCCCCACTGATAGTCAAGGT16190.15865844922390637No Hit
CCCCAGGACAAACGGCCAGGATCACCTGCTCTGGAGATGCATTGCCAATA16070.15748247554219738No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCCTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG16030.15709048431496103No Hit
GGTCAGGGACAATGGCCACCTTGACTATCAGTGGGGCCCAAGTGGAGGATGAAGGTGACTACTACTGTTATTCAA15760.15444454353111578No Hit
GGATGAAGGTGACTACTACTGTTATTCAACAGACTTCACTAATACTCGTGGGGTTTTCGGCGGTGGGACCAAG15750.1543465457243067No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA15550.152386589588125No Hit
CACTAATACTCGTGGGGTTTTCGGCGGTGGGACCAAGCTGACCGTCCTGGGTCAGCCCAAGGCTGCCCCCTCGGT15360.15052463125875243No Hit
CTGCTATGGTCATCTTTGACGACAAGGAGCGACCCTCCGGGATCCCTGAGAGAATCTCCGGCTCCAGGTCAGGGA15300.14993664441789792No Hit
ACCATATATTACGCGGACTCTGTGAAGGGCCGATTCACCGTGTCCAGGGACAATGCCAAGAACTCACTGTATCTG15070.147682694861289No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC14790.14493875627063468No Hit
GTCTATTACTGTGCGAGCGGCTTCATGGACGTCTGGGGCCAAGGGACCACGGTCACCGTCTCCTCTGCATCCCCG14240.13954887689613507No Hit
CCCCTCTCCTGCTCCCCCTCCTCACTTTCTGCACAGTCTCTGAGGCCTCC14120.13837290321442605No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA13900.1362169514646262No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT13720.13445299094206273No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC13710.13435499313525365No Hit
GACTATCAGTGGGGCCCAAGTGGAGGATGAAGGTGACTACTACTGTTATT13570.13298302383992647No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT13020.12759314446542686No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA13020.12759314446542686No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC12830.12573118613605427No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC12670.12416322122710893No Hit
CCCCTGCTATGGTCATCTTTGACGACAAGGAGCGACCCTCCGGGATCCCT12260.12014531114793651No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG12100.11857734623899117No Hit
CCTTGACTATCAGTGGGGCCCAAGTGGAGGATGAAGGTGACTACTACTGT11880.11642139448919132No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG11880.11642139448919132No Hit
GGCCTGGACCCCTCTCCTGCTCCCCCTCCTCACTTTCTGCACAGTCTCTGAGGCCTCCTATGAGCTGACACAGC11830.11593140545514591No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT11760.11524542080748232No Hit
CTGATAGTCAAGGTGGCCATTGTCCCTGACCTGGAGCCGGAGATTCTCTC11750.11514742300067324No Hit
GGATCACCTGCTCTGGAGATGCATTGCCAATAAGATATGTTTATTGGTACCATCAGAAGTCAGGCCAGGCCCCTG11680.11446143835300965No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC11650.11416744493258241No Hit
CTCTTGGTACAGCCTGGGGAGTCCCTGAGACTCTCTTGTGCAGCCTCTGG11570.11338346247810974No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT11510.11279547563725523No Hit
GTCCAGGGACAATGCCAAGAACTCACTGTATCTGCAAATGAACGGCCTGACAGACGGGGACACGGCTGTCTATTA11480.11250148221682797No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT11090.10867956775127373No Hit
ATATATGGTAGTTCCAGTGGAATTAATGAATGAAATCCACTCCAGCCCCTTCCCTGGAGCCTGGCGGACCCAGTT11010.10789558529680106No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC10990.1076995896831829No Hit
CTCCTGCTCCCCCTCCTCACTTTCTGCACAGTCTCTGAGGCCTCCTATGA10890.10671961161509205No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC10680.1046616576721013No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC10670.10456365986529222No Hit
GTTCAAGGACCACCTGACCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG10530.10319169056996504No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT10460.10250570592230145No Hit
CCTTCATCCTCCACTTGGGCCCCACTGATAGTCAAGGTGGCCATTGTCCC10450.10240770811549237No Hit
GTGCTGGGTTTTCCTTGCTGGTATTCTAGAAGGTGTCCTGTGTGAGGTGC10340.10132973224059244No Hit
GAATTGGGGCTGTGCTGGGTTTTCCTTGCTGGTATTCTAGAAGGTGTCCT10220.10015375855888346No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGTAT203.7722912E-4109.4142270
TACGAGA150.001891888772.9196964
TAACGTA150.002543754267.674626
CGTAATT150.00254424967.671319
CTTCGTA250.006252311653.92553369
CCGTGCG353.6650337E-451.35764769
GTGGTAT2350.046.0808641
TGGTATC2450.041.4334452
GGTATCA7000.039.1584431
GTATCAA17250.037.8622671
TATTACA451.12262824E-437.597012
ATCTACG400.001979667236.4598564
GTTGAAT16200.033.7698270
TCTACGG552.292032E-433.29836765
CACAATA900.003519772332.4190370
TATCAAC20400.031.8468822
ACGTCTG8550.031.777529
ATCAACG20800.031.3955843
GGACGTC8550.031.34018127
TGAGTTC10050.031.19832452
Produced by FastQC (version 0.11.5)