FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689759

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689759
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences586548
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACGGGGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGC97441.6612451154892693No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG74641.272530125411731No Hit
ATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTGCCTACCA65521.1170441293807156No Hit
CCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCT62041.0577139466846703No Hit
CTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCT54780.9339389103705067No Hit
GAGTACGGGGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTT52530.8955788784549602No Hit
GTACGGGGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTC45500.7757250898477193No Hit
GGTATCAACGCAGAGTACGGGGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGTCG44620.7607220551429721No Hit
GGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACC38580.6577466805785716No Hit
CTCCGGAATCGAACCCTGATTCCCCGTCACCCGTGGTCACCATGGTAGGC36510.6224554512162688No Hit
CTACCATGGTGACCACGGGTGACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACAT35840.6110326861569726No Hit
CGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTGCCTACCATGGTGAC34810.5934723159911891No Hit
ATCAACTTTCGATGGTAGTCGCCGTGCCTACCATGGTGACCACGGGTGAC31170.5314143088033716No Hit
GGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTT30090.5130014934839093No Hit
ATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCACTCCCGA28260.4818020008592648No Hit
GCCTACCATGGTGACCACGGGTGACGGGGAATCAGGGTTCGATTCCGGAG27880.47532341769130576No Hit
ACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTGCCT25200.4296323574541214No Hit
GCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCC24260.4136063885649598No Hit
CCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCTGATTCCCCGTCACC23350.3980918867680054No Hit
TCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCC23290.3970689525835908No Hit
GCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCTGATTCCCCGTCACCCGTGGT22780.38837401201606686No Hit
GGCTCCCTCTCCGGAATCGAACCCTGATTCCCCGTCACCCGTGGTCACCA19610.3343289892728302No Hit
ACCATGGTGACCACGGGTGACGGGGAATCAGGGTTCGATTCCGGAGAGGG19400.3307487196273792No Hit
GGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCTGATT19310.3292143183507573No Hit
TATCAACGCAGAGTACGGGGTGGCGGCGACGACCCATTCGAACGTCTGCC19010.32409964742868447No Hit
GCCCTATCAACTTTCGATGGTAGTCGCCGTGCCTACCATGGTGACCACGGGTGACGGGGAATCAGGGTTCGATTC18930.32273573518279836No Hit
CCCTCTCCGGAATCGAACCCTGATTCCCCGTCACCCGTGGTCACCATGGT18600.31710959716851816No Hit
GTAGTCGCCGTGCCTACCATGGTGACCACGGGTGACGGGGAATCAGGGTT18160.30960807981614463No Hit
CCCTGATTCCCCGTCACCCGTGGTCACCATGGTAGGCACGGCGACTACCA18000.3068802553243724No Hit
TGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCTGATTCCCCGTCACCCGTGGTCACCATGGTAGGCA17000.2898313522507962No Hit
CATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCACTCCCGACCCGGGGA16920.2884674400049101No Hit
CGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCT16440.2802839665295935No Hit
GTATCAACGCAGAGTACGGGTGGCGGCGACGACCCATTCGAACGTCTGCC16200.2761922297919352No Hit
GTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTT16120.2748283175460491No Hit
GAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATC15880.27073658080839075No Hit
GTGCCTACCATGGTGACCACGGGTGACGGGGAATCAGGGTTCGATTCCGG15480.2639170195789603No Hit
CCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCT15310.26101870605645233No Hit
GACCACGGGTGACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGAAGG14530.24772056165906284No Hit
ACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCC13950.23783219787638865No Hit
ATCAACGCAGAGTACGGGGTGGCGGCGACGACCCATTCGAACGTCTGCCC13930.2374912198149171No Hit
GGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCA13760.23459290629240914No Hit
GGTGACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGAAGGCAGCAGG13190.2248750315404707No Hit
ATCGAACCCTGATTCCCCGTCACCCGTGGTCACCATGGTAGGCACGGCGA13160.2243635644482634No Hit
ACTTTCGATGGTAGTCGCCGTGCCTACCATGGTGACCACGGGTGACGGGG12950.22078329480281236No Hit
CTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCT12910.22010133867986933No Hit
CCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCTGATTCCCCGTCACCCGTGGTCACCATGGTAGGCACGGCGA12810.21839644837251174No Hit
CTTATACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGT12630.215327645819268No Hit
GAGTACGGGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTT12420.21174737617381698No Hit
CCATGGTGACCACGGGTGACGGGGAATCAGGGTTCGATTCCGGAGAGGGA12200.2079966174976302No Hit
GGTAGTCGCCGTGCCTACCATGGTGACCACGGGTGACGGGGAATCAGGGT12150.20714417234395138No Hit
CCATGGTAGGCACGGCGACTACCATCGAAAGTTGATAGGGCAGACGTTCGAATGGGTCGTCGCCGCCACCCCGTA12120.2066327052517441No Hit
GCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAAT11950.20373439172923616No Hit
CTATCAACTTTCGATGGTAGTCGCCGTGCCTACCATGGTGACCACGGGTG11640.1984492317764275No Hit
CCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCC11370.19384602794656192No Hit
GGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAG10700.18242326288726582No Hit
GTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCTGATTCCCC10680.1820822848257943No Hit
CCCTATCAACTTTCGATGGTAGTCGCCGTGCCTACCATGGTGACCACGGG10490.17884299324181482No Hit
CGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTGCCTACCATGGTGACCACGGGTGACGGGGAATCAGGGTT10450.17816103711887177No Hit
GGTGACCACGGGTGACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCT10420.17764957002666448No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9750.16622680496736839No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGA9380.1599187108301452No Hit
CCCGTGGTCACCATGGTAGGCACGGCGACTACCATCGAAAGTTGATAGGGCAGACGTTCGAATGGGTCGTCGCCG9220.157190886338373No Hit
GTATCAACGCAGAGTACGGGGGCGGCGACGACCCATTCGAACGTCTGCCC9110.1553155070002796No Hit
TGCCTACCATGGTGACCACGGGTGACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCA8910.15190572638556435No Hit
GTGGTATCAACGCAGAGTACGGGGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGT8850.15088279220114978No Hit
CCATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTGCCTAC8770.14951887995526367No Hit
CCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTGCCTA8590.14645007740201996No Hit
GAGTACGGGGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTGCCTACCA8300.14150589551068282No Hit
CCTATCAACTTTCGATGGTAGTCGCCGTGCCTACCATGGTGACCACGGGT8280.1411649174492113No Hit
GTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACC8210.139971494234061No Hit
GGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACA7850.13383388912757355No Hit
CATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTGCCTACCATGGTGACCACGGGTGACGGGGAAT7810.13315193300463046No Hit
CGATGGTAGTCGCCGTGCCTACCATGGTGACCACGGGTGACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGC7800.13298144397389472No Hit
GAATCGAACCCTGATTCCCCGTCACCCGTGGTCACCATGGTAGGCACGGC7640.1302536194821225No Hit
CTCAGGCTCCCTCTCCGGAATCGAACCCTGATTCCCCGTCACCCGTGGTC7530.12837824014402915No Hit
GATTCCCCGTCACCCGTGGTCACCATGGTAGGCACGGCGACTACCATCGA7470.12735530595961456No Hit
GTACGGGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCG7410.1263323717752No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7370.12565041565225693No Hit
TTCGATGGTAGTCGCCGTGCCTACCATGGTGACCACGGGTGACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGC7260.12377503631416353No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT7240.12343405825269202No Hit
CCACGGGTGACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAA7160.12207014600680592No Hit
GGTATCAACGCAGAGTACGGGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGC7090.12087672279165558No Hit
CACTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGG7080.12070623376091982No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7050.12019476666871252No Hit
CTTTCGATGGTAGTCGCCGTGCCTACCATGGTGACCACGGGTGACGGGGA6870.11712596411546881No Hit
GCCTACCATGGTGACCACGGGTGACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCA6770.11542107380811117No Hit
CTCCGGAATCGAACCCTGATTCCCCGTCACCCGTGGTCACCATGGTAGGCACGGCGACTACCATCGAAAGTTGAT6620.11286373834707474No Hit
GAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGAAGGCAGCAGGCGCGCAAAT6620.11286373834707474No Hit
ACCATGGTGACCACGGGTGACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATC6440.10979493579383102No Hit
ATGGTAGTCGCCGTGCCTACCATGGTGACCACGGGTGACGGGGAATCAGG6420.1094539577323595No Hit
TCACTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTC6330.10791955645573764No Hit
GGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGA6330.10791955645573764No Hit
TTCGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTGCCTACCAT6190.10553271002543697No Hit
TTCTCAGGCTCCCTCTCCGGAATCGAACCCTGATTCCCCGTCACCCGTGGTCACCATGGTAGGCACGGCGACTAC6150.10485075390249392No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG6140.10468026487175815No Hit
CGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCTGATTCCCCGTCA6130.1045097758410224No Hit
CTCCCTCTCCGGAATCGAACCCTGATTCCCCGTCACCCGTGGTCACCATG6090.10382781971807933No Hit
CTGATTCCCCGTCACCCGTGGTCACCATGGTAGGCACGGCGACTACCATC5940.1012704842570429No Hit
TTTCTCAGGCTCCCTCTCCGGAATCGAACCCTGATTCCCCGTCACCCGTGGTCACCATGGTAGGCACGGCGACTA5920.10092950619557137No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCGTTGAACAAACGAACCTTTAATAGCGGCTGCAC5910.10075901716483562No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG3700.091.5483870
ACGTTAG258.0792635E-490.32773670
CACGTAA150.001502958277.2684161
CAAGTAC150.001818364573.64715653
CGGTTGC253.0063256E-669.3283441
TCGTTTA150.002688049366.7354932
GCTTCGT254.3517084E-665.141681
AGATCCT150.002964378865.108353
GGTTACG200.00454503958.44803262
TATGGCG900.058.35098343
GCGTGAA451.6007107E-858.1730756
TGTGCGT200.00568496255.23536353
CGAGGGG200.0058294954.88604452
TCTAGTT502.6579073E-854.551562
TCGGGTA252.7504165E-454.43000838
ATGCCGG1100.054.30643544
GACAGAT301.2824621E-554.2847251
TAAGCGC200.00648760953.42200547
TGCGTGT351.446629E-553.1898455
CTCTTAT1600.052.9276121
Produced by FastQC (version 0.11.5)