FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689762

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689762
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1514401
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTTATACAGCTCCAACAACAAGAACTACTTAGCTTGGTACCAGCAGAA76150.5028390763080585No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG72630.4795955628661101No Hit
GGGTAATATAATATTGTTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAAT71360.4712094088685889No Hit
CCTTAGGAGGCTGTCCTGGTTTCTGCTGGTACCAAGCTAAGTAGTTCTTGTTGTTGGAGCTGTATAAAACACT65570.43297647056492966No Hit
ATATTACCCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGA60860.40187506479459534No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT55820.36859457963907843No Hit
CTCCTAAGGTGCTCATTTACTGGGCATCTGCCCGGGAATCCGGGGTCCCT48030.31715509960703936No Hit
GTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCA43980.29041185260707036No Hit
ATATTATATTACCCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT41990.2772713435873325No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC38570.2546881572318032No Hit
GTATAAAACACTCTGGCTGGACTTGCAGTTGATGGTGGCCCTCTCGCCCAGAGACACAGCCAGGGAGTCTGGAGA38490.254159895562668No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG35780.23626503152071346No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC34980.2309824148293616No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC32450.2142761395429612No Hit
ATATAATATTGTTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTC32360.21368184516518413No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT29970.19790002779977034No Hit
GTTTATTACTGTCAACAATATTATATTACCCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAA29890.19737176613063515No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT28140.18581604211830288No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG27630.18244837397756605No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG26480.1748546124837477No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA25260.16679862202943607No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT25210.16646845848622657No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG24060.15887469699240822No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC24030.15867659886648253No Hit
GAGTGAAATCTGTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACCCCG23720.15662958489858367No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC23200.15319588404920495No Hit
CTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTTTTATACAGCTCCAA22980.15174316445908315No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA22060.1456681552640285No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAACAATATTATATTACC21880.14447956650847432No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTTTTATA21480.14183825816279835No Hit
GTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACCCCGGATTCCCGGGC21430.14150809461958888No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT20660.13642357605416267No Hit
CCCTTAGACTCTCCTGTGCAGCCTCTGGTTTCACTTTCAGTAACGCCTGG20620.13615944521959505No Hit
TCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACC20260.13378226770848672No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTGAGCGGGGTAAT19730.1302825341504661No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG19380.12797138934799965No Hit
AACCAGGACAGCCTCCTAAGGTGCTCATTTACTGGGCATCTGCCCGGGAA18960.1251980155850399No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT18300.12083985681467459No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC18200.12017952972825559No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT17860.11793441763443104No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG17840.11780235221714723No Hit
GTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCAC17760.11727409054801206No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA17600.11621756720974166No Hit
GGATCTCTGGTGCCTACGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGG17510.11562327283196458No Hit
CCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGC17510.11562327283196458No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTT17480.11542517470603889No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG17370.11469881491097801No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT17100.11291593177764675No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT16980.11212353927394396No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA16930.11179337573073446No Hit
CTCCTGTGCAGCCTCTGGTTTCACTTTCAGTAACGCCTGGATGAACTGGGTCCGCCAGGCTCCAGGGAAGGGG16700.1102746234319708No Hit
GTCCTGGTTTCTGCTGGTACCAAGCTAAGTAGTTCTTGTTGTTGGAGCTG16590.10954826363690989No Hit
GTAATACACGGCTGTGTCCTCGGTTTTCAGGCTGCTCATTTGCAGATACA16430.10849174029863952No Hit
GTCAGGGACCCCGGATTCCCGGGCAGATGCCCAGTAAATGAGCACCTTAGGAGGCTGTCCTGGTTTCTGCTGGTA16370.10809554404678813No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG16330.10783141321222055No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT16300.10763331508629485No Hit
GTGTATTACTGTACCACGGTCAAGGCGTACTATGAAAGTAGTGGGTATTTCTCAAACTGGTTCGACCCCTGGGGC15890.104925974031977No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA15880.1048599413233351No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC15730.10386945069370662No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15590.10294499277272004No Hit
GTATCAACGCAGAGTACGGGGAGCTACAACAGGCAGGCAGGGGCAGCAAG15240.10063384797025358No Hit
GGCTGAAGATGTGGCAGTTTATTACTGTCAACAATATTATATTACCCCGC15230.10056781526161168No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGATCG150.002409196268.6102437
GACAGGT2950.044.766731
GGTATCA13950.038.838151
CTAACGC350.001477942638.6968843
GTGGTAT3800.038.3175431
TGGTATC4550.034.2273372
GTGCTCA35400.034.10679670
CTACGTA400.002800550833.97199627
ACCGTAT400.002849114433.8530711
ATCAACG37750.032.4695053
TATCAAC38050.032.38722
AATGGTA4050.032.32612270
TCAACGC37850.032.202684
AACGCAG38900.031.7686426
CAACGCA38700.031.670365
CCGATCA1652.3259727E-630.85675270
CCTAACG450.005069953430.0936032
CGCAGAG41350.029.968228
ACGCAGA41650.029.7523657
AGAGTAC42850.028.91533711
Produced by FastQC (version 0.11.5)