FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004689763

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689763
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1210152
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAGACAGCCGAGTCCTCGACTCTCAGGCTGCTCATTTGCAGATACA51800.4280454025610006No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT42380.3502039413230735No Hit
GTCTATTACTGTGCAAGGGATTACGATCTTTGGCCACCTAAATACTACTA41460.3426015905439978No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC38400.31731551077881126No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGACTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA31160.25748831551738954No Hit
GTCTGGGACAGAGTTCACTCTCACCATCAGCAGTCTGCAGTCTGAAGATT28620.23649921662733278No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG27800.22972320832424356No Hit
GTCCCAGACCCACCGCCACTGAACCTGGCTGGGAGACCAGTGACCCTGCT26720.2207987095835895No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC26690.22055080684079356No Hit
GAGTTCACTCTCACCATCAGCAGTCTGCAGTCTGAAGATTTTGCAGTTTATTACTGTCAGCAGCATAGTGACTGG24450.20204073537869624No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC24120.19931380520794081No Hit
GTAGTAAGAGTGGTCACATCTACTACGCAGACTCGGTGAGGGGCCGATTC22810.18848871877251783No Hit
CTCCTGCAGGGCCAGTCAGAATATTGGCAACAACGTAGCCTGGTACCAGCAAGTCCCTGGCCAGTCTCCCAGCCT22320.18443964064018403No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG21410.176919924108707No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT21340.17634148437551647No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG21310.17609358163272051No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG20750.1714660637671962No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA20750.1714660637671962No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG20730.1713007952719989No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT19920.16460742121650834No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG19840.16394634723571913No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA19690.16270683352173942No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC19640.16229366228374617No Hit
GTCTGTGTCTCCAGGGGAAAGAGTCACCCTCTCCTGCAGGGCCAGTCAGAATATTGGCAACAACGTAGCCTGGTA19460.16080624582697048No Hit
GTCTCCAGCCACCCTGTCTGTGTCTCCAGGGGAAAGAGTCACCCTCTCCTGCAGGGCCAGTCAGAATATTGGCAA19360.15997990335098403No Hit
CTCTTCCTCCTGCTACTCTGGCTCCCAGATACCACCGGAGAAATAGTGATGACGCAGTCTCCAGCCACCCTGT19090.1577487786658205No Hit
CTCCTGTGCAGCCTCCGGATTCAGCTTCAGTGACTATAGCATGAATTGGGTCCGCCAGGCTCCAGGGAAGGGG18630.15394760327628265No Hit
GCATAGTGACTGGCCTCTGGCGTTCGGCCAGGGGACCAAGGTGCAACTCAAACGAACTGTGGCTGCACCAT18350.15163384434352048No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT17950.14832847443957453No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT17830.14733686346839076No Hit
GTTTATTACTGTCAGCAGCATAGTGACTGGCCTCTGGCGTTCGGCCAGGGGACCAAGGTGCAACTCAAACGAA17630.14568417851641777No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT17320.14312251684085966No Hit
GAACCATGGAAGCCCCAGCGCAGACTCTCTTCCTCCTGCTACTCTGGCTC16890.13956924419411776No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG16840.13915607295612453No Hit
GAGTGAACTCTGTCCCAGACCCACCGCCACTGAACCTGGCTGGGAGACCA16740.13832973048013802No Hit
CTCTTACTACTAATGCCTGAGACCCACTCCAGCCCCTTCCCTGGAGCCTGGCGGACCCAATTCATGCTATAGTCA16510.1364291427853691No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT16500.13634650853777047No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT16040.13254533314823264No Hit
CCTAAATACTACTACTACGGTATGGATGTCTGGGGCCAGGGGACCCCAGT16040.13254533314823264No Hit
CCATGGAACTGGGGCTCCGCTGGGTTTTCCTTGTTGGTATTTTAGAAGGT15860.13105791669145694No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC15740.13006630572027317No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC15520.12824835227310288No Hit
GTTCAAGGACCACCTGACCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG15280.1262651303307353No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGAGTTGCACCTTGGTCC15250.12601722758793937No Hit
GGGGAAAGAGTCACCCTCTCCTGCAGGGCCAGTCAGAATATTGGCAACAACGTAGCCTGGTACCAGCAAGTCC15230.12585195909274208No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG15080.12461244537876233No Hit
GTGCAGCCACAGTTCGTTTGAGTTGCACCTTGGTCCCCTGGCCGAACGCC14980.12378610290277585No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA14970.1237034686551772No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14500.1198196590180407No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGT14430.11924121928485017No Hit
GTAATAAACTGCAAAATCTTCAGACTGCAGACTGCTGATGGTGAGAGTGAACTCTGTCCCAGACCCACCGCCA14140.11684482610448935No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT13960.11535740964771367No Hit
CTCCCAGCCTCCTCATCTATCGAGCAACCAGCAGGGTCACTGGTCTCCCA13630.11263047947695827No Hit
GCACTGAGCACCGGGGACTCACCATGGAACTGGGGCTCCGCTGGGTTTTC13570.11213467399136638No Hit
GCTCAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGACTCTCT13470.11130833151537989No Hit
GATCAGCACTGAGCACCGGGGACTCACCATGGAACTGGGGCTCCGCTGGG13400.11072989178218934No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT13320.11006881780140015No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC13210.10915984107781503No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC13030.10767242462103935No Hit
CTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCACCGGAGAAATAGTGATGACGCAGTCTCCAGCCACC12950.10701135064025015No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC12850.10618500816426367No Hit
GGCCAGGGACTTGCTGGTACCAGGCTACGTTGTTGCCAATATTCTGACTGGCCCTGCAGGAGAGGGTGACTCTTT12810.10585447117386906No Hit
GTTCAGTGGCGGTGGGTCTGGGACAGAGTTCACTCTCACCATCAGCAGTC12710.10502812869788258No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG12570.1038712492315015No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT12570.1038712492315015No Hit
GAGTCACCCTCTCCTGCAGGGCCAGTCAGAATATTGGCAACAACGTAGCCTGGTACCAGCAAGTCCCTGGCCAGT12530.1035407122411069No Hit
GCCTTAGCCCTGGATTCCAAGGCCTCTCCACTTGGTGATCAGCACTGAGC12320.10180539304153527No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC12220.10097905056554879No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)