FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689764

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689764
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1210152
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAGACAGCCGAGTCCTCGACTCTCAGGCTGCTCATTTGCAGATACA43890.36268171271046945No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT42920.3546661906934005No Hit
GTCTATTACTGTGCAAGGGATTACGATCTTTGGCCACCTAAATACTACTA42800.3536745797222167No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG36820.30425929965822474No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGACTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA29240.24162253997844899No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC28990.23955668378848274No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC28060.23187169876180844No Hit
GTCTGGGACAGAGTTCACTCTCACCATCAGCAGTCTGCAGTCTGAAGATT28010.23145852752381518No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC26210.2165843629560584No Hit
GAGTTCACTCTCACCATCAGCAGTCTGCAGTCTGAAGATTTTGCAGTTTATTACTGTCAGCAGCATAGTGACTGG26180.21633646021326247No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG24670.20385868882586652No Hit
GTCCCAGACCCACCGCCACTGAACCTGGCTGGGAGACCAGTGACCCTGCT24280.20063595316951918No Hit
CTCCTGCAGGGCCAGTCAGAATATTGGCAACAACGTAGCCTGGTACCAGCAAGTCCCTGGCCAGTCTCCCAGCCT22170.18320012692620433No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT21670.17906841454627187No Hit
GTAGTAAGAGTGGTCACATCTACTACGCAGACTCGGTGAGGGGCCGATTC21640.1788205118034759No Hit
GTCTGTGTCTCCAGGGGAAAGAGTCACCCTCTCCTGCAGGGCCAGTCAGAATATTGGCAACAACGTAGCCTGGTA19700.1627894677693381No Hit
CTCCTGTGCAGCCTCCGGATTCAGCTTCAGTGACTATAGCATGAATTGGGTCCGCCAGGCTCCAGGGAAGGGG19260.15915356087499752No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC18990.156922436189834No Hit
GTCTCCAGCCACCCTGTCTGTGTCTCCAGGGGAAAGAGTCACCCTCTCCTGCAGGGCCAGTCAGAATATTGGCAA18880.15601345946624887No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG18690.15444340876187454No Hit
CTCTTCCTCCTGCTACTCTGGCTCCCAGATACCACCGGAGAAATAGTGATGACGCAGTCTCCAGCCACCCTGT18650.15411287177147995No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT18650.15411287177147995No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG18630.15394760327628265No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA18430.15229491832430966No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT18220.15055959912473807No Hit
GTTTATTACTGTCAGCAGCATAGTGACTGGCCTCTGGCGTTCGGCCAGGGGACCAAGGTGCAACTCAAACGAA17500.14460993329763536No Hit
GAACCATGGAAGCCCCAGCGCAGACTCTCTTCCTCCTGCTACTCTGGCTC17260.14262671135526775No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT17010.14056085516530153No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG16880.13948660994651912No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT16800.13882553596572994No Hit
GTTCAAGGACCACCTGACCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG16790.13874290171813128No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA16610.1372554852613556No Hit
GCATAGTGACTGGCCTCTGGCGTTCGGCCAGGGGACCAAGGTGCAACTCAAACGAACTGTGGCTGCACCAT16430.13576806880457992No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG16410.13560280030938263No Hit
GAGTGAACTCTGTCCCAGACCCACCGCCACTGAACCTGGCTGGGAGACCA16200.13386748110981098No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG15930.1316363564246475No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA15750.1301489399678718No Hit
GTGCAGCCACAGTTCGTTTGAGTTGCACCTTGGTCCCCTGGCCGAACGCC15560.12857888926349748No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC15410.12733937554951774No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT14940.12345556591238126No Hit
GGGGAAAGAGTCACCCTCTCCTGCAGGGCCAGTCAGAATATTGGCAACAACGTAGCCTGGTACCAGCAAGTCC14830.12254658918879613No Hit
CTCTTACTACTAATGCCTGAGACCCACTCCAGCCCCTTCCCTGGAGCCTGGCGGACCCAATTCATGCTATAGTCA14600.1206460014940272No Hit
GTAATAAACTGCAAAATCTTCAGACTGCAGACTGCTGATGGTGAGAGTGAACTCTGTCCCAGACCCACCGCCA14410.11907595078965287No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC13720.1133741877053461No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT13600.11238257673416231No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGAGTTGCACCTTGGTCC13430.1109777945249853No Hit
CCTAAATACTACTACTACGGTATGGATGTCTGGGGCCAGGGGACCCCAGT13230.10932510957301232No Hit
GAGTCACCCTCTCCTGCAGGGCCAGTCAGAATATTGGCAACAACGTAGCCTGGTACCAGCAAGTCCCTGGCCAGT13050.10783769311623664No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT13010.10750715612584205No Hit
GTTCAGTGGCGGTGGGTCTGGGACAGAGTTCACTCTCACCATCAGCAGTC12990.10734188763064474No Hit
CTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCACCGGAGAAATAGTGATGACGCAGTCTCCAGCCACC12930.10684608214505285No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC12830.10601973966906637No Hit
CTCCCAGCCTCCTCATCTATCGAGCAACCAGCAGGGTCACTGGTCTCCCA12720.10511076294548122No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG12700.10494549445028392No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG12670.10469759170748799No Hit
GTCACTATGCTGCTGACAGTAATAAACTGCAAAATCTTCAGACTGCAGACTGCTGATGGTGAGAGTGAACT12620.10428442046949474No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT12590.1040365177266988No Hit
GGCCAGGGACTTGCTGGTACCAGGCTACGTTGTTGCCAATATTCTGACTGGCCCTGCAGGAGAGGGTGACTCTTT12560.10378861498390286No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA12480.10312754100311365No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT12220.10097905056554879No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT12210.10089641631795014No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGGAG13250.054.89288370
GGTATCA9450.039.9252431
CTCTGGA14150.039.14126269
GTATCAA23950.036.8998221
CGTTGTC12700.034.7374863
CTCATTT13700.032.99810435
GTTGTCT13450.032.9109764
TCATTTG13750.032.8959236
GCGTTGT13400.032.82548562
GGCGTTG13750.032.4297161
ATTTGCA13900.032.36858438
TGTCTCT14450.032.17304666
TGAGTTC13750.032.08513652
TATCAAC27700.031.9083122
GATACAC13800.031.90744445
GCAGATA14000.031.83017742
TTGCAGA14100.031.526340
TGGGCGT14000.031.40779959
ATCAACG27950.031.259323
TCAACGC28200.030.984764
Produced by FastQC (version 0.11.5)