FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689765

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689765
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1331567
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATTATACTGTTGGCAGTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTC117640.8834703773824374No Hit
GTATAATAGTTATCCGAACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT108300.8133274555467356No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT54570.40981790627133297No Hit
GAGTATTAGTAGCTGGTTGGCCTGGTATCAGCAGGAACCAGGGAAAGTCCCTAAACTCCTCATCTATAAGGCGT54350.4081657175343036No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC47790.35890045337560933No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC34590.2597691291538466No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG32070.24084405816605547No Hit
TCTATAAGGCGTCTAGTTTAGAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGGGGATCTGGGACAGAATTCACT31900.23956736686926006No Hit
GTTTAGGGACTTTCCCTGGTTCCTGCTGATACCAGGCCAACCAGCTACTA31630.2375396806919967No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC31630.2375396806919967No Hit
GTCCCTAAACTCCTCATCTATAAGGCGTCTAGTTTAGAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGGGGAT30990.23273331345700218No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA30590.22972933393513054No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG29440.22109289280974972No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC28130.21125485987562023No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG28070.2108042629473395No Hit
GAATTCACTCTCACCATCAGCAGCCTGCAGCCTGATGATTTTGCAACTTATTACTGCCAACAGTATAATAGTTAT27670.2078002834254679No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG27160.20397020953508158No Hit
CCCTCCACCCTGTCTGCATCTGTAGGAGATAGAGTCACCATCACTTGCCG27120.20366981158289446No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT25240.18955110783009793No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG25080.18834951602134928No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA24670.1852704370114309No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT24650.1851202380353373No Hit
GTCTGCATCTGTAGGAGATAGAGTCACCATCACTTGCCGGGCCAGTCAGA24200.18174076107323175No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC24150.18136526363299782No Hit
GTATTAGTAGCTGGTTGGCCTGGTATCAGCAGGAACCAGGGAAAGTCCCT23850.1791122789915941No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT23710.17806088615893906No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG22280.16732165936824808No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACAT21390.1606378049320838No Hit
CCTTATAGATGAGGAGTTTAGGGACTTTCCCTGGTTCCTGCTGATACCAGGCCAACCAGCTACTAATACTCTGA20990.15763382541021217No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT20700.15545594025685527No Hit
CAGTATAATAGTTATCCGAACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT20480.1538037515198259No Hit
GGCCAAAAGTGTTCGGATAACTATTATACTGTTGGCAGTAATAAGTTGCA20240.15200136380670293No Hit
CTAATACTCTGACTGGCCCGGCAAGTGATGGTGACTCTATCTCCTACAGA20100.15094997097404786No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT19720.14809619042826985No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA18410.13825815749414036No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC18110.13600517285273667No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACATCCAGATGACCCAGTCTCCCTCCACC17760.133376690771099No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC17500.1314241040818825No Hit
GTAGGAGATAGAGTCACCATCACTTGCCGGGCCAGTCAGAGTATTAGTAGCTGGTTGGCCTGGTATCAGCAGGAA17280.1297719153448531No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT16720.12556634401423286No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG16400.12316316039673557No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG16360.12286276244454841No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG15520.11655440544861807No Hit
GAGATAGAGTCACCATCACTTGCCGGGCCAGTCAGAGTATTAGTAGCTGG15500.11640420647252447No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC15290.11482711722354189No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG15110.11347532643869968No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC15030.11287453053432535No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT14860.11159783923752993No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG14610.10972035203636017No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT14390.10806816329933079No Hit
GAGTGAATTCTGTCCCAGATCCCCTGCCGCTGAACCTTGATGGGACCCCA14330.10761756637105006No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT14330.10761756637105006No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT14310.10746736739495648No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT14050.10551478070573991No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG13810.10371239299261698No Hit
GTCTCCCTCCACCCTGTCTGCATCTGTAGGAGATAGAGTCACCATCACTTGCCGGGCCAGTCAGAGTATTAGTAG13590.1020602042555876No Hit
ACTTATTACTGCCAACAGTATAATAGTTATCCGAACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAA13490.10130920937511968No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA13420.10078351295879216No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTGCGA250.006631909453.12916669
CGTATGC2750.045.20601341
TCGTATG2900.045.18567740
GTCGTAT651.1545126E-842.3948839
ATCTCCG4350.039.15879410
GTATCAA35450.039.0335271
GTATGCC3250.038.29059242
GCCGTCT2800.037.2156946
TATACAC4800.036.908553
ACGAGAC4450.036.76700222
CTAACGC754.4916305E-836.340733
GACGGAT400.002085965136.07431465
TCTCCGA4550.035.94003311
CCCACGA4600.035.55872319
ATCTCGT2750.034.98048837
CACGAGA4550.034.45805721
ATTCTGT28900.034.21623268
CCCTAAG4650.033.76649528
CATCTCG2950.033.7479836
ATCAGGC28600.033.6096336
Produced by FastQC (version 0.11.5)