FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689767

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689767
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1101318
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATTATACTACTCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT105440.9573983172889211No Hit
GTTTTATACAGCTCCACCAATAGGAACTACTTAGCTTGGTACCAGCAGAA73210.6647489644226281No Hit
GTATAATAGTGTTGACAGAAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTC63450.5761278758723638No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT43530.3952536869460047No Hit
GTATAAAACACTCTGGCTGGACTTGCAGTTGATGGTGGCCCTCTCGCCCAGAGACACAGCCAAGGAGTCTGGAGA39860.3619299784440098No Hit
ATACTACTCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGA37630.3416815125150048No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC37490.3404103083759641No Hit
GAGTAGTATAATAGTGTTGACAGAAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAAT35110.3187998380122726No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC31020.28166251709315565No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG28600.2596888455468811No Hit
CTCCTTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTTTTATACAGCTCCAC25940.23553596690510822No Hit
TCTTAGGAGGCTGTCCTGGTTTCTGCTGGTACCAAGCTAAGTAGTTCCTATTGGTGGAGCTGTATAAAACACT25410.2307235512358828No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT24330.22091711930614047No Hit
ACCCGGGACTCCGGGGTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAGCAGC23470.21310829388060487No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG22630.2054810690463608No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT22580.20502706756813202No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG22200.20157665633359304No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA21760.19758144332517943No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG20950.1902266193778727No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG20720.18813821257802016No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC20480.18595900548252187No Hit
ATTATACTACTCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAA19990.1815097909958795No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG19750.17933058390038117No Hit
CTCCTAAGATGCTCATTTACTGGGCATCTACCCGGGACTCCGGGGTCCCT19620.17815018005698627No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT19400.17615257355277947No Hit
AACCAGGACAGCCTCCTAAGATGCTCATTTACTGGGCATCTACCCGGGAC18930.17188495965742864No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC18630.16916095078805576No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC17610.15989932063218798No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTTCTGTCAACACTATTATACTACT17190.15608570821506595No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT17160.15581330732812865No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA16790.15245369638923542No Hit
GTCTCCAGACTCCTTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTTTTATA15950.1448264715549914No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT15530.14101285913786935No Hit
CTACTTAGCTTGGTACCAGCAGAAACCAGGACAGCCTCCTAAGATGCTCATTTACTGGGCATCTACCCGGGACTC15170.1377440484946219No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15070.13683604553816425No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT15030.1364728443555812No Hit
GTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCAC14880.1351108399208948No Hit
GTAATACACGGCTGTGTCCTCGGTTTTCAGGCTATTCATTTGCAGATACA14830.13465683844266596No Hit
CTTATACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGT14390.13066162543425242No Hit
CCTTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGC14250.12939042129521172No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG13920.12639401153890156No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTT13880.12603081035631852No Hit
GTCCCGGGTAGATGCCCAGTAAATGAGCATCTTAGGAGGCTGTCCTGGTTTCTGCTGGTACCAAGCTAAGTAGTT13630.12376080296517444No Hit
GGACTGAACACAGAGGACTCACCATGGAGTTCGGGCTGAGCTGGATTTTC13610.12357920237388294No Hit
GTAGTATAATAGTGTTGACAGAAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAAT13360.12130919498273887No Hit
GTCAGGGACCCCGGAGTCCCGGGTAGATGCCCAGTAAATGAGCATCTTAGGAGGCTGTCCTGGTTTCTGCTGGTA13260.12040119202628125No Hit
GGATCTCTGGTGCCTACGGGGACATCGTGATGACCCAGTCTCCAGACTCCTTGGCTGTGTCTCTGGGCGAGAGGG13120.11912998788724057No Hit
GTAAATGAGCATCTTAGGAGGCTGTCCTGGTTTCTGCTGGTACCAAGCTA13110.11903918759159479No Hit
GTCCTGGTTTCTGCTGGTACCAAGCTAAGTAGTTCCTATTGGTGGAGCTG12980.1178587837481999No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG12970.11776798345255411No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC12880.11695078079174226No Hit
ACTTAGCTTGGTACCAGCAGAAACCAGGACAGCCTCCTAAGATGCTCATT12740.1156795766527016No Hit
CCCTTAGACTCTCCTGTGCAGCCTCTGGATTCGCTTTCAGTAACGCCTGG12730.1155887763570558No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA12680.11513477487882702No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA12650.11486237399188971No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA12520.11368197014849481No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC12260.11132116246170497No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC12120.1100499583226643No Hit
GTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTTTTATACAGCTCCACCAATAGGAACTA12080.10968675714008126No Hit
GAACTACTTAGCTTGGTACCAGCAGAAACCAGGACAGCCTCCTAAGATGCTCATTTACTGGGCATCTACCCGGGA12080.10968675714008126No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT11940.10841555300104057No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC11930.10832475270539481No Hit
ATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAGCAGCC11920.10823395240974905No Hit
GTTTATTTCTGTCAACACTATTATACTACTCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAA11910.10814315211410327No Hit
CATTTACTGGGCATCTACCCGGGACTCCGGGGTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCA11830.10741674974893717No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT11810.10723514915764566No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA11710.10632714620118804No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA11660.10587314472295922No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC11480.10423873940133548No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT11020.10006192580163042No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC11020.10006192580163042No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTAAG150.002376583968.845240
CGCGGTT208.6662345E-568.7534539
CTACCTT400.00656406953.2638570
TCGCGGT200.007504584351.4940938
CGTATGG552.6138878E-950.14538241
TCGTATG3400.048.5966140
CACGAGA8400.047.31650521
CGTATGC3500.047.27993441
ACGAGAC8600.047.01681522
CTTATAC8450.046.642971
ATCTCGT4050.046.5774137
GAGACTA8750.046.21920824
TATACAC8700.046.0628173
TGCCGTC3300.045.1842945
CGAGACT9100.044.43750823
CATCTCG4800.044.26291336
TACACAT9000.044.150045
GGTATCA8500.043.9702261
CGAGCCC9150.043.79743615
TGCGTGA901.8189894E-1243.09942255
Produced by FastQC (version 0.11.5)