FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004689771

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689771
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences854725
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCCACTCTGTGTCGGAGTCTCCGGGGAAGACGGTAACCATCTCCTGCACCGGCAGCAGTGGCAACATTGCCAG38160.4464593875223025No Hit
GACTTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCCTTGATCGGTGCTATCATAAGACTGACAGTAGTAGT31800.37204948960191875No Hit
GTGTCGGAGTCTCCGGGGAAGACGGTAACCATCTCCTGCACCGGCAGCAGTGGCAACATTGCCAGCAACTATGTG30280.35426599198572645No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG27750.3246658281903536No Hit
TCTCTGGACTGAAGACTGAGGACGAGGCTGACTACTACTGTCAGTCTTAT26990.31577407938225743No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC26690.3122641785369563No Hit
GCCTTGGGCTGACTTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCCTTGATCGGTGCTATCATAAGACTGA25510.2984585685454386No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT22840.2672204510222586No Hit
ATCATAAGACTGACAGTAGTAGTCAGCCTCGTCCTCAGTCTTCAGTCCAG22820.2669864576325719No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACTTAGGACGGTCAGCTTGGTCCCTC21800.2550527947585481No Hit
GTGCTATCATAAGACTGACAGTAGTAGTCAGCCTCGTCCTCAGTCTTCAG21780.25481880136886137No Hit
GTCTTATGATAGCACCGATCAAGGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAAGTCAGCCCAAGGCTGC21390.2502559302699699No Hit
GTCCAGAGATGGTGAGGGAGGCAGAGTTGGAGGAGCTGTCGATGGAGCCA20880.24428909883295796No Hit
GACTACTACTGTCAGTCTTATGATAGCACCGATCAAGGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAAGT20460.2393752376495364No Hit
CCCCTTGATCGGTGCTATCATAAGACTGACAGTAGTAGTCAGCCTCGTCC20370.23832226739594606No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA20350.23808827400625932No Hit
CTGCACAGGTTCTTGGGCCAATTTTATGCTGACTCAGCCCCACTCTGTGTCGGAGTCTCCGGGGAAGACGGTAAC19070.2231126970663079No Hit
TGATAGCACCGATCAAGGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCC18930.22147474333850067No Hit
GTAGTAGTCAGCCTCGTCCTCAGTCTTCAGTCCAGAGATGGTGAGGGAGG18650.2181988358828863No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC18440.2157419052911755No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT18380.2150399251221153No Hit
GTAATATATGGCCGTGTCCGCAGCGGTCACAGAGCTCAGCTTCAGGGAGA18290.21398695486852495No Hit
TCCTAAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG18180.21269999122524788No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG17960.21012606393869374No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG17830.20860510690572992No Hit
ATCAAGGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAAGTCAGCCC17830.20860510690572992No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA17240.20170230190997102No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC17200.20123431513059759No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG17070.19971335809763371No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT16020.1874287051390798No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG15330.17935593319488724No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA15260.17853695633098365No Hit
GTCCTAAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC15030.17584603234958612No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA14840.17362309514756208No Hit
ATTTTATGCTGACTCAGCCCCACTCTGTGTCGGAGTCTCCGGGGAAGACG14830.1735060984527187No Hit
CAGTAGTAGTCAGCCTCGTCCTCAGTCTTCAGTCCAGAGATGGTGAGGGA14710.17210213811459826No Hit
ACCAAAGACCCTCTGGGGTCCCCGATCGTTTCTCTGGCTCCATCGACAGC14690.17186814472491152No Hit
CTCCTGCACCGGCAGCAGTGGCAACATTGCCAGCAACTATGTGCAGTGGTACCAGCAGCGCCCGGGCAGTGCCCC14610.17093217116616455No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT14580.17058118108163445No Hit
CTACTACTGTCAGTCTTATGATAGCACCGATCAAGGGGTGTTCGGCGGAG14460.16917722074351402No Hit
CTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGT14180.16590131328789962No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG13810.16157243557869488No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA13750.16087045540963468No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACTTAGGACGGTCAGCTTGGTCCCTCCGCCGAACAC13670.1599344818508877No Hit
CCACTACTTCTCACCCTCCTCGCTCACTGCACAGGTTCTTGGGCCAATTT12790.14963877270467107No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC12780.14952177600982772No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG12630.14776682558717716No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT12580.14718184211296031No Hit
GAGTTGGAGGAGCTGTCGATGGAGCCAGAGAAACGATCGGGGACCCCAGA12560.14694784872327357No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC12270.1435549445728158No Hit
CCACTATGGCCTGGGCTCCACTACTTCTCACCCTCCTCGCTCACTGCACAGGTTCTTGGGCCAATTTTATGCTGA12000.14039603381204482No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC11930.1395770569481412No Hit
GCCTGGAGCATCTCCACTATGGCCTGGGCTCCACTACTTCTCACCCTCCT11750.13747111644096055No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC11680.13665213957705694No Hit
GTCTCTGGTGGCTCCATCAGTACTTACTACTGGAGCTGGATCCGGCAGCC11490.13442920237503292No Hit
GTGGTACCAGCAGCGCCCGGGCAGTGCCCCCACCACTGTGATCTATGAGGATAACCAAAGACCCTCTGGGGTCCC11380.13314223873175582No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT10740.1256544502617801No Hit
CCTCCAACTCTGCCTCCCTCACCATCTCTGGACTGAAGACTGAGGACGAG10630.12436748661850304No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC10560.12354850975459944No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG10440.12214454941647898No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA10190.11921963204539472No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC10170.11898563865570798No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG10160.11886864196086461No Hit
ACTATGTGCAGTGGTACCAGCAGCGCCCGGGCAGTGCCCCCACCACTGTGATCTATGAGGATAACCAAAGACCCT10030.11734768492790079No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA10020.11723068823305742No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT9780.11442276755681653No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA9780.11442276755681653No Hit
CTTCTCACCCTCCTCGCTCACTGCACAGGTTCTTGGGCCAATTTTATGCT9710.11360379069291293No Hit
GTCTTTGGTTATCCTCATAGATCACAGTGGTGGGGGCACTGCCCGGGCGCTGCTGGTACCACTGCACATAGTTG9690.11336979730322619No Hit
CCCCAGAGGGTCTTTGGTTATCCTCATAGATCACAGTGGTGGGGGCACTGCCCGGGCGCTGCTGGTACCACTGCA9660.11301880721869607No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACTTAGGACGGTCAGCTTGGTC9550.11173184357541899No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGC9480.1109128667115154No Hit
CTCCACTACTTCTCACCCTCCTCGCTCACTGCACAGGTTCTTGGGCCAATTTTATGCTGACTCAGCCCCACT9460.11067887332182864No Hit
GCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGC9310.1089239228991781No Hit
GTCTTCAGTCCAGAGATGGTGAGGGAGGCAGAGTTGGAGGAGCTGTCGATGGAGCCAGAGAAACGATCGGGGACC9250.10822194273011787No Hit
GAGTGGGGCTGAGTCAGCATAAAATTGGCCCAAGAACCTGTGCAGTGAGCGAGGAGGGTGAGAAGTAGTGGAGCC9230.10798794934043113No Hit
GCACAGGTTCTTGGGCCAATTTTATGCTGACTCAGCCCCACTCTGTGTCG9210.10775395595074438No Hit
GGTTCTTGGGCCAATTTTATGCTGACTCAGCCCCACTCTGTGTCGGAGTCTCCGGGGAAGACGGTAACCATCTC9130.10681798239199741No Hit
GGCCTGGGCTCCACTACTTCTCACCCTCCTCGCTCACTGCACAGGTTCTTGGGCCAATTTTATGCTGACTCAGCC9110.1065839890023107No Hit
GGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG8910.10424405510544327No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG8710.10190412120857585No Hit
CTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCC8600.10061715756529879No Hit
GCTTGGTCCCTCCGCCGAACACCCCTTGATCGGTGCTATCATAAGACTGA8590.10050016087045542No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)