FastQCFastQC Report
Wed 8 May 2024
EGAF00004689772

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689772
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences854725
Total Bases62.8 Mbp
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG47680.5578402410131913No Hit
GCCCCACTCTGTGTCGGAGTCTCCGGGGAAGACGGTAACCATCTCCTGCA38330.44844833133463985No Hit
GACTTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCCTTGATCGGTG35110.4107753955950744No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG34070.3986077393313639No Hit
GTGTCGGAGTCTCCGGGGAAGACGGTAACCATCTCCTGCACCGGCAGCAG33910.39673579221387No Hit
TCTCTGGACTGAAGACTGAGGACGAGGCTGACTACTACTGTCAGTCTTAT31060.36339173418350934No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC30190.3532130217321361No Hit
GTCTTATGATAGCACCGATCAAGGGGTGTTCGGCGGAGGGACCAAGCTGA27880.32618678522331745No Hit
GCCTTGGGCTGACTTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCC27610.32302787446254644No Hit
GTGCTATCATAAGACTGACAGTAGTAGTCAGCCTCGTCCTCAGTCTTCAG26970.3155400859925707No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT25790.30173447600105296No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA25430.29752259498669165No Hit
ATCATAAGACTGACAGTAGTAGTCAGCCTCGTCCTCAGTCTTCAGTCCAG25310.2961186346485712No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC24370.2851209453332943No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT23510.27505922957676443No Hit
GACTACTACTGTCAGTCTTATGATAGCACCGATCAAGGGGTGTTCGGCGG23420.27400625932317413No Hit
TGATAGCACCGATCAAGGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCC22720.26581649068413815No Hit
CCCCTTGATCGGTGCTATCATAAGACTGACAGTAGTAGTCAGCCTCGTCC22410.2621895931439937No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT21700.25388282781011434No Hit
GTCCAGAGATGGTGAGGGAGGCAGAGTTGGAGGAGCTGTCGATGGAGCCA21690.253765831115271No Hit
CTGCACAGGTTCTTGGGCCAATTTTATGCTGACTCAGCCCCACTCTGTGT21360.24990494018543977No Hit
GTAGTAGTCAGCCTCGTCCTCAGTCTTCAGTCCAGAGATGGTGAGGGAGG21290.24908596332153618No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAG21150.24744800959372895No Hit
ACCAAAGACCCTCTGGGGTCCCCGATCGTTTCTCTGGCTCCATCGACAGC20580.24077919798765687No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCA20530.24019421451344No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACA20380.23843926409078944No Hit
TCCTAAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCC20270.23715230044751237No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA20240.23680131036298227No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT19750.2310684723156571No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACTTAGGACGG19610.22943051858784988No Hit
GTAATATATGGCCGTGTCCGCAGCGGTCACAGAGCTCAGCTTCAGGGAGA19370.226622597911609No Hit
ATCAAGGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAAGTCAGCCC19060.22299570037146452No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTT19000.2222937202024043No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCA18420.21550791190148877No Hit
GTCCTAAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC17530.2050952060604288No Hit
ATTTTATGCTGACTCAGCCCCACTCTGTGTCGGAGTCTCCGGGGAAGACG17360.2031062622480915No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA17320.20263827546871802No Hit
CTACTACTGTCAGTCTTATGATAGCACCGATCAAGGGGTGTTCGGCGGAG17040.19936236801310364No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCT16810.1966714440317061No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA16340.19117259937406766No Hit
CTCCTGCACCGGCAGCAGTGGCAACATTGCCAGCAACTATGTGCAGTGGT16230.18988563573079062No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGT16020.1874287051390798No Hit
GTGGTACCAGCAGCGCCCGGGCAGTGCCCCCACCACTGTGATCTATGAGG15740.18415279768346543No Hit
CAGTAGTAGTCAGCCTCGTCCTCAGTCTTCAGTCCAGAGATGGTGAGGGA15240.1783029629412969No Hit
GAGTTGGAGGAGCTGTCGATGGAGCCAGAGAAACGATCGGGGACCCCAGA15220.17806896955161017No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA14560.1703471876919477No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC14430.1688262306589839No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT14360.1680072537950803No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT14190.166018309982743No Hit
GGTACCAGCAGCGCCCGGGCAGTGCCCCCACCACTGTGATCTATGAGGAT14000.16379537278071896No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC13780.1612214454941648No Hit
GTCTTTGGTTATCCTCATAGATCACAGTGGTGGGGGCACTGCCCGGGCGC13690.16016847524057445No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC13410.15689256778496008No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA13390.15665857439527334No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTG13350.15619058761589985No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTC13200.1544356371932493No Hit
GTCTCTGGTGGCTCCATCAGTACTTACTACTGGAGCTGGATCCGGCAGCC13110.15338266693965893No Hit
GAGTGGGGCTGAGTCAGCATAAAATTGGCCCAAGAACCTGTGCAGTGAGC13000.1520957032963819No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT12590.1472988388078037No Hit
GTCTTCAGTCCAGAGATGGTGAGGGAGGCAGAGTTGGAGGAGCTGTCGAT12540.14671385533358683No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACTTAGGACGGTCAGCTTGGTC12440.14554388838515311No Hit
CCTCCAACTCTGCCTCCCTCACCATCTCTGGACTGAAGACTGAGGACGAG12290.14378893796250256No Hit
CCCCAGAGGGTCTTTGGTTATCCTCATAGATCACAGTGGTGGGGGCACTG11890.13910907016876772No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG11870.13887507677908098No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTG11600.13571616601830996No Hit
GCACAGGTTCTTGGGCCAATTTTATGCTGACTCAGCCCCACTCTGTGTCG11590.1355991693234666No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGA11510.13466319576471963No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTG11440.13384421890081605No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT11420.1336102255111293No Hit
CCACTATGGCCTGGGCTCCACTACTTCTCACCCTCCTCGCTCACTGCACA11230.1313872883091053No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACAC11150.13045131475035832No Hit
GGTTCTTGGGCCAATTTTATGCTGACTCAGCCCCACTCTGTGTCGGAGTC11110.12998332797098483No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA10960.12822837754833427No Hit
GCCTGGAGCATCTCCACTATGGCCTGGGCTCCACTACTTCTCACCCTCCT10800.12635643043084033No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGG10790.12623943373599697No Hit
ACTATGTGCAGTGGTACCAGCAGCGCCCGGGCAGTGCCCCCACCACTGTG10760.12588844365146684No Hit
GGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACTTAGGACGGT10460.12237854280616574No Hit
GCTTGGTCCCTCCGCCGAACACCCCTTGATCGGTGCTATCATAAGACTGA10320.12074058907835852No Hit
GTGCCCCCACCACTGTGATCTATGAGGATAACCAAAGACCCTCTGGGGTC10320.12074058907835852No Hit
GGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG10250.11992161221445494No Hit
CTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCC10080.11793266840211764No Hit
ATATATTACTGTGCGAGAACTAAGAAATACTATGATAGTAGTGGTCCCCA9960.1165287080639972No Hit
CCACTACTTCTCACCCTCCTCGCTCACTGCACAGGTTCTTGGGCCAATTT9940.11629471467431045No Hit
CCAAAGACCCTCTGGGGTCCCCGATCGTTTCTCTGGCTCCATCGACAGCT9860.11535874111556349No Hit
GGCCTGGGCTCCACTACTTCTCACCCTCCTCGCTCACTGCACAGGTTCTT9470.11079587001667202No Hit
CCCCACCACTGTGATCTATGAGGATAACCAAAGACCCTCTGGGGTCCCCG9470.11079587001667202No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCA9450.1105618766269853No Hit
ACCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAAAAGCTACAGCTGC9330.10915791628886484No Hit
CCCTCTGGGGTCCCCGATCGTTTCTCTGGCTCCATCGACAGCTCCTCCAA9290.10868992950949134No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGA9290.10868992950949134No Hit
GCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGC9240.1081049460352745No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACATCTGTGGTTCTTCCTTCT9120.10670098569715407No Hit
GTGGTGGGGGCACTGCCCGGGCGCTGCTGGTACCACTGCACATAGTTGCT9100.10646699230746733No Hit
CCTCTGGGGTCCCCGATCGTTTCTCTGGCTCCATCGACAGCTCCTCCAAC8970.1049460352745035No Hit
CCACTGTGATCTATGAGGATAACCAAAGACCCTCTGGGGTCCCCGATCGT8920.10436105180028664No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACA8900.1041270584105999No Hit
GGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTG8890.10401006171575652No Hit
GGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCC8880.10389306502091315No Hit
CTATGAGGATAACCAAAGACCCTCTGGGGTCCCCGATCGTTTCTCTGGCT8840.10342507824153968No Hit
CGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC8800.1029570914621662No Hit
ACTATGGCCTGGGCTCCACTACTTCTCACCCTCCTCGCTCACTGCACAGG8720.10202111790341922No Hit
GGACCAAGCTGACCGTCCTAAGTCAGCCCAAGGCTGCCCCCTCGGTCACT8710.10190412120857585No Hit
CTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCCCCG8640.10108514434467226No Hit
GTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTT8640.10108514434467226No Hit
GGACGAGGCTGACTACTACTGTCAGTCTTATGATAGCACCGATCAAGGGG8610.10073415426014216No Hit
GAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACTTAG8600.10061715756529879No Hit
CTCCGACACAGAGTGGGGCTGAGTCAGCATAAAATTGGCCCAAGAACCTG8560.10014917078592529No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)