FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004689773

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689773
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1856011
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAGACAGCCGTGTCCTCGGCGCTCAGGTTGTTCATCAGAAGATGCA88780.47833768226589174No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT66060.35592461467092595No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC60890.328069176314149No Hit
GTCTAATAATCCGTGGACGTTCGGCCAGGGGACCAAGGTGGATAAGAAGCGAACTGTGGCTGCACCATCTGTCTT59020.3179938049936127No Hit
CTCCTGTACAGCCTCGGGATTCAGCGTCAGTTCTTACTACATGAGCTGGG57380.3091576504665113No Hit
GTCTATTACTGTGCAAGAGTTGGTAACAGACTTGCTAATATGCGCCTTTA51240.2760759499808999No Hit
GTATAAACACGAGTCAGGAAAAGCCCCTCAACTCCTGATCCACGCCGCTA44020.23717531846524617No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC43430.23399645799513044No Hit
TTATTAGACTGTTGACAATAGTAAATTGCAAAATCTTCAGGCTGCAGTCTACTGATGGTGAGAGTGAAGTTTGTC42610.2295783807315797No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC38830.20921212212643134No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG38020.20484792385389955No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG37620.20269276421314314No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG37160.20021433062627322No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG36210.19509582647947668No Hit
CTATTATGCAGACTCCGTGAAGGGCAGATTCGTCATCTCCAGAGACAATT34780.18739113076377242No Hit
TCAGTAGACTGCAGCCTGAAGATTTTGCAATTTACTATTGTCAACAGTCT34490.185828640024224No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG34450.18561312406014835No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC33240.17909376614686012No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA32260.17381362502700684No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC32170.17332871410783665No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT32100.17295156117070426No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT32030.1725744082335719No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT30100.16217576296692207No Hit
GATCTGCACTGAGCACAGAGGACTCGCCATGGAGTTTGGGCTGAGCTGGG30090.16212188397590316No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG29100.15678786386503096No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA28890.15565640505363385No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGGTTCCAGATGCGACAT27720.14935256310442127No Hit
GATTATTAGACTGTTGACAATAGTAAATTGCAAAATCTTCAGGCTGCAGT26300.1417017463797359No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGT26210.1412168354605657No Hit
GATCAGGAGTTGAGGGGCTTTTCCTGACTCGTGTTTATACCAGCCTAACC25700.13846900691860126No Hit
CTCCTGATCCACGCCGCTACCAATTTACAGAGTGGGGTGTCGCCAAGGTTCAGCGGCTGGGGATCTGGGACAAA25560.1377147010443365No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT25450.13712203214312846No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT24650.13281171286161558No Hit
GTCTCCATCTCTCGTGTCTGCCTCTGTTGGAGACAAAGTCACCATGACCTGCCGGGCGAGTCAGAATATTGGCAG24580.13243455992448322No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC24220.1304949162478024No Hit
GGTTAGGCTGGTATAAACACGAGTCAGGAAAAGCCCCTCAACTCCTGATC24010.12936345743640526No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT23690.12763932972380013No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG23530.12677726586749755No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA23390.12602295999323282No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGGTTCCAGATGCGACATCC23050.12419107429858982No Hit
GCATAATAGTCACCACCGCCGGTTTCAATCAGTGAGACCCACTCCGGCCTCTTACCTGGAGCCTGGCGGACCCAG22750.1225747045680225No Hit
GTCTACTGATGGTGAGAGTGAAGTTTGTCCCAGATCCCCAGCCGCTGAAC22730.12246694658598467No Hit
CTAATAATCCGTGGACGTTCGGCCAGGGGACCAAGGTGGATAAGAAGCGA22260.11993463400809587No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC21720.11702516849307466No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT21450.11557043573556408No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG21440.11551655674454517No Hit
GAATATTGGCAGCTGGTTAGGCTGGTATAAACACGAGTCAGGAAAAGCCCCTCAACTCCTGATCCACGCCGCTAC21260.11454673490620476No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG21000.11314588113971308No Hit
CCTTGGTCCCCTGGCCGAACGTCCACGGATTATTAGACTGTTGACAATAG20940.1128226071935996No Hit
GAGTTTGGGCTGAGCTGGGTTTTCCTTGTTGCTGTTTTAAAAGGTGTCCAATGTGATGAACAGCTGATGGAGT20890.11255321223850505No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG20690.11147563241812683No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG20690.11147563241812683No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCATGCG19710.10619549129827356No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT19660.10592609634317901No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG19640.10581833836114118No Hit
ATTGTCAACAGTCTAATAATCCGTGGACGTTCGGCCAGGGGACCAAGGTGGATAAGAAGCGAACTGTGGCTGCAC19630.10576445937012227No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT19070.10274723587306324No Hit
GAGTGGGGTGTCGCCAAGGTTCAGCGGCTGGGGATCTGGGACAAACTTCACTCTCACCATCAGTAGACTGCAGC18980.10226232495389306No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT18760.10107698715147702No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)