FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689774

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689774
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1856011
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAGACAGCCGTGTCCTCGGCGCTCAGGTTGTTCATCAGAAGATGCA79460.4281224626362667No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT66310.35727158944639875No Hit
GTCTAATAATCCGTGGACGTTCGGCCAGGGGACCAAGGTGGATAAGAAGCGAACTGTGGCTGCACCATCTGTCTT57390.30921152945753017No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG56420.3039852673286958No Hit
CTCCTGTACAGCCTCGGGATTCAGCGTCAGTTCTTACTACATGAGCTGGG55420.2985973682268047No Hit
GTCTATTACTGTGCAAGAGTTGGTAACAGACTTGCTAATATGCGCCTTTA49540.266916521507685No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC47470.25576357036677044No Hit
TTATTAGACTGTTGACAATAGTAAATTGCAAAATCTTCAGGCTGCAGTCTACTGATGGTGAGAGTGAAGTTTGTC44210.23819901929460546No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC43840.23620549662690576No Hit
GTATAAACACGAGTCAGGAAAAGCCCCTCAACTCCTGATCCACGCCGCTA41300.22252023290810236No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC41140.22165816905179977No Hit
CTATTATGCAGACTCCGTGAAGGGCAGATTCGTCATCTCCAGAGACAATT33530.18065625688640854No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG33320.17952479807501143No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG32480.1749989628294229No Hit
TCAGTAGACTGCAGCCTGAAGATTTTGCAATTTACTATTGTCAACAGTCT32090.17289768217968535No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC31400.1691800317993805No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT31250.16837184693409682No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT31010.167078751149643No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG30800.16594729233824584No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA30280.16314558480526245No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG30110.16222964195794098No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT29410.1584581125866172No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC28970.15608743698178512No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG26470.14261768922705736No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA26370.1420788993168683No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGGTTCCAGATGCGACAT26020.1401931346312064No Hit
GATTATTAGACTGTTGACAATAGTAAATTGCAAAATCTTCAGGCTGCAGT25160.13555954140358004No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG25080.13512850947542876No Hit
GCATAATAGTCACCACCGCCGGTTTCAATCAGTGAGACCCACTCCGGCCTCTTACCTGGAGCCTGGCGGACCCAG24660.1328655918526345No Hit
CTCCTGATCCACGCCGCTACCAATTTACAGAGTGGGGTGTCGCCAAGGTTCAGCGGCTGGGGATCTGGGACAAA24380.131356980104105No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT24220.1304949162478024No Hit
GTCTCCATCTCTCGTGTCTGCCTCTGTTGGAGACAAAGTCACCATGACCTGCCGGGCGAGTCAGAATATTGGCAG23680.1275854507327812No Hit
GTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAACTTCCCACCTAGCCAGGATGCCTCCGGGGAC23660.1274776927507434No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT23400.1260768389842517No Hit
GATCAGGAGTTGAGGGGCTTTTCCTGACTCGTGTTTATACCAGCCTAACC23370.125915202011195No Hit
GATCTGCACTGAGCACAGAGGACTCGCCATGGAGTTTGGGCTGAGCTGGG23360.12586132302017605No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT23350.12580744402915714No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC23310.12559192806508152No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGT23230.12516089613693024No Hit
GTCTACTGATGGTGAGAGTGAAGTTTGTCCCAGATCCCCAGCCGCTGAAC22560.12155100373866319No Hit
GGTTAGGCTGGTATAAACACGAGTCAGGAAAAGCCCCTCAACTCCTGATC21290.1147083718792615No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT20920.11271484921156179No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG20670.111367874436089No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA20600.11099072149895664No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG20550.11072132654386209No Hit
ATTGTCAACAGTCTAATAATCCGTGGACGTTCGGCCAGGGGACCAAGGTGGATAAGAAGCGAACTGTGGCTGCAC20380.1098053836965406No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGGTTCCAGATGCGACATCC20180.10872780387616238No Hit
CTAATAATCCGTGGACGTTCGGCCAGGGGACCAAGGTGGATAAGAAGCGA20090.10824289295699217No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA19700.10614161230725465No Hit
GAATATTGGCAGCTGGTTAGGCTGGTATAAACACGAGTCAGGAAAAGCCCCTCAACTCCTGATCCACGCCGCTAC19670.1059799753341979No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT19650.1058722173521601No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG19160.10323214679223344No Hit
CCTTGGTCCCCTGGCCGAACGTCCACGGATTATTAGACTGTTGACAATAG18990.10231620394491198No Hit
GTGTTTATACCAGCCTAACCAGCTGCCAATATTCTGACTCGCCCGGCAGG18960.10215456697185524No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT18610.10026880228619335No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGGAG24150.051.43799670
GGTATCA14950.043.4073221
CCGGGAA19650.040.5886370
GTGGTAT4300.039.5068471
CTCTGGA24700.039.0684869
TGGTATC4500.038.5091172
CGTTTAA550.006931095438.4987970
GACAGGT3750.038.0529941
GTATCAA36550.037.64771
AATAGAC23250.034.783373
TCGGCGC22450.034.71399720
AGGTTGT22550.034.667729
TAGACAG23650.034.339675
TAATAGA23450.034.1959842
ATAGACA23500.034.1250654
CCGTGTC22800.034.11846512
CGGCGCT22750.034.1140121
AGCCGTG23350.034.05047610
CGTGTTG22600.034.02221352
GGAATTG23150.033.93942361
Produced by FastQC (version 0.11.5)