FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689777

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689777
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2377369
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCA101180.42559653129152436No Hit
GTAATAGTCAGCCTCATCGTCAGACTGGAGCCCAGAGATGAGTAAAATCC99650.41916084545562765No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC94700.3983395089277264No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAAGCGCTGCTGTGCCAAATCATACAGTAATAGTCAG81460.3426476916288553No Hit
GACTATTACTGTATGATTTGGCACAGCAGCGCTTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAG79030.332426308242431No Hit
GTAATATACAGCCGTGTCCTCGTCTCTCAGGCTGTTCATTTGCAGATACA78540.3303652062426994No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG70230.29541059885949555No Hit
GTGCTGACTCAGCCGTCTTCCCTCTCTGCATCTCCTGGAGCATCAGCCAG69330.291624901308968No Hit
CACCTGCACCTTACGCAGTGACATCAATATTGATACCTTCAGGATATACTGGTACCAGCAGAAGCCAGGGAGTC63770.2682377031079315No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC61000.25658616731353023No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT59870.25183301372231237No Hit
CTCCAGAGCCCTGCTGCTTATCTGAGTCTGATTTGTACCTCAGGAGATACTGGGGAGGACTCCCTGGCTTCTG58490.24602827747817022No Hit
GCTCTGGAGTCCCCAGCCGCTTCTCTGGATCCAAAGATGCTTCGGCCAATGCAGGGATTTTACTCATCTCTGGG58460.24590208755981927No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG57600.2422846432337597No Hit
GGCCTGGACTCCTCTCCTCCTCCTGTTCCTCTCTCACTGCACAGGTTCCC52250.21978077446117958No Hit
GGATATACTGGTACCAGCAGAAGCCAGGGAGTCCTCCCCAGTATCTCCTG51310.2158268236861842No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC50570.21271413903352823No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA48980.20602607336092965No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC47960.20173561613699853No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG47640.2003895903412554No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT45850.1928602585463174No Hit
CCCCAGTATCTCCTGAGGTACAAATCAGACTCAGATAAGCAGCAGGGCTC45100.18970551058754445No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT45080.18962138397531053No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG44780.18835948479180134No Hit
GGTTCCCTCTCGCAGGCTGTGCTGACTCAGCCGTCTTCCCTCTCTGCATCTCCTGGAGCATCAGCCAGTCTCAC44400.1867610791593564No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC43910.18469997715962477No Hit
GTACGGGACTGCGGGGGTAAGAGGTTGTGTCCACCATGGCCTGGACTCCTCTCCTCCTCCTGTTCCTCTCTCA43610.1834380779761156No Hit
GTATATTACTGTGCGACGATTAAAGATACAACTATGGCCCCGGACGTCTGGGGCCAAGGGACCACGGTCACCGT43350.18234443201707434No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG43260.18196586226202158No Hit
TCTCTGGGCTCCAGTCTGACGATGAGGCTGACTATTACTGTATGATTTGG42990.18083015299686334No Hit
GGTAAGAGGTTGTGTCCACCATGGCCTGGACTCCTCTCCTCCTCCTGTTCCTCTCTCACTGCACAGGTTCCCTCT42640.17935793728276933No Hit
CAGTAATAGTCAGCCTCATCGTCAGACTGGAGCCCAGAGATGAGTAAAAT42640.17935793728276933No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG41980.17658175907904913No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA41890.1762031893239964No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA41850.1760349360995285No Hit
CATCAATATTGATACCTTCAGGATATACTGGTACCAGCAGAAGCCAGGGAGTCCTCCCCAGTATCTCCTGAGGTA40240.16926274381469597No Hit
CTCGCAGGCTGTGCTGACTCAGCCGTCTTCCCTCTCTGCATCTCCTGGAG39890.16779052810060197No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAAGCGCTGCTGTGCCAAATCATACAGTAATAGTCAGC39310.1653508563458176No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG38830.16333181765220292No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG37950.15963024671390935No Hit
CAGATAAGCAGCAGGGCTCTGGAGTCCCCAGCCGCTTCTCTGGATCCAAA37710.15862072736710203No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC37550.15794771446923048No Hit
GTGCAGGTGAGACTGGCTGATGCTCCAGGAGATGCAGAGAGGGAAGACGG37170.15634930883678552No Hit
CTATTACTGTATGATTTGGCACAGCAGCGCTTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCC37070.1559286757756158No Hit
ATACTGGGGAGGACTCCCTGGCTTCTGCTGGTACCAGTATATCCTGAAGG35920.151091395572164No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT35800.15058663589876037No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA35500.14932473671525118No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA35300.14848347059291175No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACAC35130.14776839438892322No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA34430.1448239629607352No Hit
GTATATGGTCATACTATTACCAGTAATGTATGAAACCCACTCCAGCCCCTTCCCTGGAGCCTGGCGGACCCAGTT34280.14419301336898058No Hit
ACCATATACTACGCAGACTCTGTGAAGGGCCGATTCACCATCTCCAGAGACAATGCCCAGAACTCACTGTATCTG34140.14360412708334297No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC33660.1415850883897283No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG33170.13952398638999666No Hit
CTCACTGCACAGGTTCCCTCTCGCAGGCTGTGCTGACTCAGCCGTCTTCCCTCTCTGCATCTCCTGGAGCATCAG33040.13897716341047603No Hit
CCCAAGCGCTGCTGTGCCAAATCATACAGTAATAGTCAGCCTCATCGTCA32870.13826208720648753No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG32340.136032731982288No Hit
CCTCTCCTCCTCCTGTTCCTCTCTCACTGCACAGGTTCCCTCTCGCAGGCTGTGCTGACTCAGCCGTCTTCCCT32100.13502321263548064No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC30890.12993355259532702No Hit
GCACAGGTTCCCTCTCGCAGGCTGTGCTGACTCAGCCGTCTTCCCTCTCTGCATCTCCTGGAGCATCAGCCAGT30410.12791451390171235No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC29590.12446532280012063No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT29520.12417087965730182No Hit
GTATCTCCTGAGGTACAAATCAGACTCAGATAAGCAGCAGGGCTCTGGAG29330.12337167684107937No Hit
GAGTACGGGACTGCGGGGGTAAGAGGTTGTGTCCACCATGGCCTGGACTCCTCTCCTCCTCCTGTTCCTCTCTCA28820.12122644822911378No Hit
GTATCAACGCAGAGTACGGGACTGCGGGGGTAAGAGGTTGTGTCCACCATGGCCTGGACTCCTCTCCTCCTC28620.12038518210677435No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG28440.11962804259666884No Hit
GTATATCCTGAAGGTATCAATATTGATGTCACTGCGTAAGGTGCAGGTGA28340.11920740953549913No Hit
GATTTGTACCTCAGGAGATACTGGGGAGGACTCCCTGGCTTCTGCTGGTA28340.11920740953549913No Hit
ATCCTGAAGGTATCAATATTGATGTCACTGCGTAAGGTGCAGGTGAGACT27920.11744075067858628No Hit
GTACAAATCAGACTCAGATAAGCAGCAGGGCTCTGGAGTCCCCAGCCGCTTCTCTGGATCCAAAGATGCTTCGGC27370.11512726884215282No Hit
GTCTCACCTGCACCTTACGCAGTGACATCAATATTGATACCTTCAGGATA27280.11474869908710007No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC27150.11420187610757943No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC27040.11373917974029273No Hit
CTCCTGGAGCATCAGCCAGTCTCACCTGCACCTTACGCAGTGACATCAAT26990.11352886320970788No Hit
CTGCACAGGTTCCCTCTCGCAGGCTGTGCTGACTCAGCCGTCTTCCCTCT26940.11331854667912301No Hit
GCTTGGTCCCTCCGCCGAACACCCAAGCGCTGCTGTGCCAAATCATACAGTAATAGTCAGCCTCATCGTCAGA26930.11327648337300603No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAAGCGCTG26870.11302410353630421No Hit
CTTCAGGATATACTGGTACCAGCAGAAGCCAGGGAGTCCTCCCCAGTATC26810.11277172369960238No Hit
GCTTATCTGAGTCTGATTTGTACCTCAGGAGATACTGGGGAGGACTCCCTGGCTTCTGCTGGTACCAGTATATC26630.11201458418949688No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT26480.1113836345977423No Hit
ATGCAGGGATTTTACTCATCTCTGGGCTCCAGTCTGACGATGAGGCTGAC26310.11066855839375377No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG26300.11062649508763679No Hit
ATATTGATACCTTCAGGATATACTGGTACCAGCAGAAGCCAGGGAGTCCTCCCCAGTATCTCCTGAGGTACAAAT26030.10949078582247855No Hit
ATATTGATGTCACTGCGTAAGGTGCAGGTGAGACTGGCTGATGCTCCAGG25790.10848126647567122No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA25510.10730349390439599No Hit
TCGTCAGACTGGAGCCCAGAGATGAGTAAAATCCCTGCATTGGCCGAAGC25360.10667254431264142No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA25250.10620984794535472No Hit
GCCCAGAGATGAGTAAAATCCCTGCATTGGCCGAAGCATCTTTGGATCCAGAGAAGCGGCTGGGGACTCCAGAGC25210.10604159472088683No Hit
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT24800.10431699917009098No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT24590.10343366974163454No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT24500.1030550999865818No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC24330.10234002378259328No Hit
CTCCTGAGGTACAAATCAGACTCAGATAAGCAGCAGGGCTCTGGAGTCCC24010.10099399798685017No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGCAT200.006341475553.73623362
GCGAATA200.007765401651.05286423
AGGGGGG4150.050.37868570
GTGGTAT3700.042.3274421
TGGTATC3700.041.3924752
TACGGAC451.0854428E-437.8153462
TCTCGTA4050.037.33942438
GGATGGG15650.037.31363770
CTCTTAT1750.036.9642141
CGTATGC4450.036.44563341
CTTATAC4600.036.2663761
GACAGGC3000.035.180851
AATCTCG4300.035.0969636
ATCTCGT4450.034.71780437
TATACAC5000.034.7144853
ATGCCGT4650.034.27854544
GGCAATC4500.034.20576533
TCGTATG4850.034.1083840
TAACGTA400.002775450234.03524423
TTATACA4950.033.6900372
Produced by FastQC (version 0.11.5)