FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689778

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689778
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2377369
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCA99800.4197917950473822No Hit
GTAATAGTCAGCCTCATCGTCAGACTGGAGCCCAGAGATGAGTAAAATCC93780.3944696847649649No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC90060.3788221348894513No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAAGCGCTGCTGTGCCAAATCATACAGTAATAGTCAG80500.33860961424162594No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG78010.32813585101849985No Hit
GACTATTACTGTATGATTTGGCACAGCAGCGCTTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAG73440.3089129201230436No Hit
GTGCTGACTCAGCCGTCTTCCCTCTCTGCATCTCCTGGAGCATCAGCCAG72100.3032764371033693No Hit
GTAATATACAGCCGTGTCCTCGTCTCTCAGGCTGTTCATTTGCAGATACA70050.29465345934939No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG64040.26937341237308976No Hit
CTCCAGAGCCCTGCTGCTTATCTGAGTCTGATTTGTACCTCAGGAGATACTGGGGAGGACTCCCTGGCTTCTG63670.26781707004676175No Hit
CACCTGCACCTTACGCAGTGACATCAATATTGATACCTTCAGGATATACTGGTACCAGCAGAAGCCAGGGAGTC60600.25490363506885133No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG56550.2378679960914776No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT56370.23711085658137207No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC56060.23580689409174593No Hit
GCTCTGGAGTCCCCAGCCGCTTCTCTGGATCCAAAGATGCTTCGGCCAATGCAGGGATTTTACTCATCTCTGGG55720.2343767416837689No Hit
GGCCTGGACTCCTCTCCTCCTCCTGTTCCTCTCTCACTGCACAGGTTCCC54880.23084342396994326No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC48920.20577369352422784No Hit
GGATATACTGGTACCAGCAGAAGCCAGGGAGTCCTCCCCAGTATCTCCTG48420.20367052821837922No Hit
GGTTCCCTCTCGCAGGCTGTGCTGACTCAGCCGTCTTCCCTCTCTGCATCTCCTGGAGCATCAGCCAGTCTCAC47720.20072609679019116No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG47180.1984546782598747No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC46640.19618325972955816No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACAC46610.19605706981120724No Hit
GTACGGGACTGCGGGGGTAAGAGGTTGTGTCCACCATGGCCTGGACTCCTCTCCTCCTCCTGTTCCTCTCTCA46530.19572056336227148No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG46100.19391184119924168No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT45100.18970551058754445No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC45090.18966344728142748No Hit
GGTAAGAGGTTGTGTCCACCATGGCCTGGACTCCTCTCCTCCTCCTGTTCCTCTCTCACTGCACAGGTTCCCTCT44850.18865392793462016No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG44730.1881491682612165No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA44340.1865086993226546No Hit
GTATATTACTGTGCGACGATTAAAGATACAACTATGGCCCCGGACGTCTGGGGCCAAGGGACCACGGTCACCGT43270.18200792556813855No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG43240.18188173564978766No Hit
CTCGCAGGCTGTGCTGACTCAGCCGTCTTCCCTCTCTGCATCTCCTGGAG42750.17982063365005602No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG42460.17860079777266383No Hit
CCCCAGTATCTCCTGAGGTACAAATCAGACTCAGATAAGCAGCAGGGCTC42140.17725477197692072No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA41470.17443653046708357No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT40920.1721230486306501No Hit
TCTCTGGGCTCCAGTCTGACGATGAGGCTGACTATTACTGTATGATTTGG40710.1712397192021937No Hit
CAGTAATAGTCAGCCTCATCGTCAGACTGGAGCCCAGAGATGAGTAAAAT40250.16930480712081297No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA40240.16926274381469597No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG38640.16253261483598044No Hit
CATCAATATTGATACCTTCAGGATATACTGGTACCAGCAGAAGCCAGGGAGTCCTCCCCAGTATCTCCTGAGGTA38080.16017706969342999No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAAGCGCTGCTGTGCCAAATCATACAGTAATAGTCAGC35960.1512596487966319No Hit
CTATTACTGTATGATTTGGCACAGCAGCGCTTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCC35240.1482310907562099No Hit
GTATATGGTCATACTATTACCAGTAATGTATGAAACCCACTCCAGCCCCTTCCCTGGAGCCTGGCGGACCCAGTT35180.14797871091950807No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG35160.14789458430727415No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA34970.14709538149105167No Hit
CCTCTCCTCCTCCTGTTCCTCTCTCACTGCACAGGTTCCCTCTCGCAGGCTGTGCTGACTCAGCCGTCTTCCCT33990.1429731774915884No Hit
GTGCAGGTGAGACTGGCTGATGCTCCAGGAGATGCAGAGAGGGAAGACGG33610.14137477185914346No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA33570.14120651863467557No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA33090.1391874799410609No Hit
CCCAAGCGCTGCTGTGCCAAATCATACAGTAATAGTCAGCCTCATCGTCA32750.13775732753308384No Hit
CAGATAAGCAGCAGGGCTCTGGAGTCCCCAGCCGCTTCTCTGGATCCAAA32600.13712637794132926No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT32470.13657955496180862No Hit
ATACTGGGGAGGACTCCCTGGCTTCTGCTGGTACCAGTATATCCTGAAGG32190.1354017823905334No Hit
CTCACTGCACAGGTTCCCTCTCGCAGGCTGTGCTGACTCAGCCGTCTTCCCTCTCTGCATCTCCTGGAGCATCAG31940.1343501997376091No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC31870.1340557565947903No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC31730.1334668703091527No Hit
GTATCAACGCAGAGTACGGGACTGCGGGGGTAAGAGGTTGTGTCCACCATGGCCTGGACTCCTCTCCTCCTC31330.1317843380644738No Hit
GAGTACGGGACTGCGGGGGTAAGAGGTTGTGTCCACCATGGCCTGGACTCCTCTCCTCCTCCTGTTCCTCTCTCA30990.13035418565649676No Hit
ACCATATACTACGCAGACTCTGTGAAGGGCCGATTCACCATCTCCAGAGACAATGCCCAGAACTCACTGTATCTG30930.13010180581979489No Hit
GTATCTCCTGAGGTACAAATCAGACTCAGATAAGCAGCAGGGCTCTGGAG30380.12778832398336143No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC30080.12652642479985227No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC29620.12459151271847155No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC29460.1239184998206No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA29340.12341374014719633No Hit
GCACAGGTTCCCTCTCGCAGGCTGTGCTGACTCAGCCGTCTTCCCTCTCTGCATCTCCTGGAGCATCAGCCAGT29310.12328755022884541No Hit
GCTTGGTCCCTCCGCCGAACACCCAAGCGCTGCTGTGCCAAATCATACAGTAATAGTCAGCCTCATCGTCAGA29300.12324548692272844No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG29260.12307723369826057No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT28760.12097406839241195No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAAGCGCTG28650.12051137202512525No Hit
CTCCTGGAGCATCAGCCAGTCTCACCTGCACCTTACGCAGTGACATCAAT28300.11903915631103124No Hit
GTACAAATCAGACTCAGATAAGCAGCAGGGCTCTGGAGTCCCCAGCCGCTTCTCTGGATCCAAAGATGCTTCGGC27260.11466457247486612No Hit
ATATTGATACCTTCAGGATATACTGGTACCAGCAGAAGCCAGGGAGTCCTCCCCAGTATCTCCTGAGGTACAAAT27220.11449631925039823No Hit
CTGCACAGGTTCCCTCTCGCAGGCTGTGCTGACTCAGCCGTCTTCCCTCT26980.1134867999035909No Hit
GATTTGTACCTCAGGAGATACTGGGGAGGACTCCCTGGCTTCTGCTGGTA26960.11340267329135696No Hit
GTCTCACCTGCACCTTACGCAGTGACATCAATATTGATACCTTCAGGATA26650.11209871080173081No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG26390.11100506484268956No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG26110.10982729227141433No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC25940.10911221606742579No Hit
ATGCAGGGATTTTACTCATCTCTGGGCTCCAGTCTGACGATGAGGCTGAC25900.1089439628429579No Hit
GTATATCCTGAAGGTATCAATATTGATGTCACTGCGTAAGGTGCAGGTGA25880.10885983623072397No Hit
GCTTATCTGAGTCTGATTTGTACCTCAGGAGATACTGGGGAGGACTCCCTGGCTTCTGCTGGTACCAGTATATC25830.1086495197001391No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT25170.10587334149641894No Hit
CTTCAGGATATACTGGTACCAGCAGAAGCCAGGGAGTCCTCCCCAGTATC24960.10499001206796252No Hit
ATCCTGAAGGTATCAATATTGATGTCACTGCGTAAGGTGCAGGTGAGACT24960.10499001206796252No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC24830.1044431890884419No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT24710.10393842941503822No Hit
TCGTCAGACTGGAGCCCAGAGATGAGTAAAATCCCTGCATTGGCCGAAGC24680.1038122394966873No Hit
GGGCTCTGGAGTCCCCAGCCGCTTCTCTGGATCCAAAGATGCTTCGGCCA24530.10318128990493272No Hit
CTCCTCCTCCTGTTCCTCTCTCACTGCACAGGTTCCCTCTCGCAGGCTGTGCTGACTCAGCCGTCTTCCCTCT24500.1030550999865818No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA24080.10128844112966898No Hit
GGGGTAAGAGGTTGTGTCCACCATGGCCTGGACTCCTCTCCTCCTCCTGTTCCTCTCTCACTGCACAGGTTCCCT23890.1004892383134465No Hit
GGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG23840.10027892178286164No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC23830.10023685847674467No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCCG656.270077E-446.06118470
GTGGTAT4900.045.0915681
GACAGGT3700.041.341751
TGGTATC5600.040.0621262
GGATGGG16750.036.19584770
TATCAAC43450.033.0924342
GACAGGC3700.033.0734021
AATATAC25400.031.448733
ATCAACG46150.031.229363
TCAACGC46200.031.1200244
CGTCTCT24850.031.06486921
TAATATA25800.030.9618052
AGGTACG553.555791E-430.8993134
GACTCGC553.560619E-430.89216411
CAACGCA46650.030.8913925
GTAATAT25900.030.8422581
ATTTGCA25700.030.73663938
CAGATAC25600.030.54218543
TCATTTG25500.030.51136636
AACGCAG47150.030.4917226
Produced by FastQC (version 0.11.5)