FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004689785

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689785
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1069633
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC51070.477453481708212No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCAG40740.38087830124912003No Hit
GTCCTGGGCCCAGTCTGTACTGACTCAGCCACCCTCAGCGTCTGGGGCCC36970.34563256743200704No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG36490.34114504694600856No Hit
GTACTGACTCAGCCACCCTCAGCGTCTGGGGCCCCCGGGCAGAGGGTCAC30940.28925809132665126No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT30560.2857054709419025No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA29200.27299082956490683No Hit
AGTCTGTACTGACTCAGCCACCCTCAGCGTCTGGGGCCCCCGGGCAGAGG29080.2718689494434072No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC28660.2679423690181586No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCAGACCATTCAGA28590.2672879389472838No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG28390.2654181387447844No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC27920.2610241082689109No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTACTGACTCAGCCACCCTCAGCGTCT27740.2593412880866615No Hit
GATTATTATTGTGCTGCATGGGATGACAGTCTGAATGGTCTGGTATTCGG26170.24466335649704152No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT26000.24307402632491706No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC25220.23578180553516953No Hit
GGATGACAGTCTGAATGGTCTGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCC24570.2297049548770466No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG24390.22802213469479718No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG23850.2229736741480489No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG23360.2183926636519255No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC22060.20623896233567962No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT21940.20511708221418No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG21830.20408869210280536No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA21470.2007230517383065No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG21410.2001621116775567No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT20870.19511365113080842No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA20830.19473969109030856No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG19380.18118363962218817No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC18110.16931040833631722No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA17630.16482288785031876No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCAGACCATTCAGAC17600.16454241781994383No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA17310.16183120752631977No Hit
ATAATAATCAGCCTCATCCTCAGACTGGAGCCCACTGATGGCCAGGGAGG17270.1614572474858199No Hit
CTGTCATCCCATGCAGCACAATAATAATCAGCCTCATCCTCAGACTGGAG17230.16108328744532002No Hit
GTCTGAGGATGAGGCTGATTATTATTGTGCTGCATGGGATGACAGTCTGAATGGTCTGGTATTCGGCGGAGGGAC17170.16052234738457022No Hit
CCCCTGAGGGCCGCTGATCATTCTTATAGATGAGGAGTGAGGGGGCCGATCCTGGGAATTGCTGGTACCAATTGA17160.16042885737444526No Hit
GTCTGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCC17100.15986791731369543No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC16970.15865254718207086No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTACTGACTCAGCCAC16450.15379106665557252No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGT16370.15304314657457277No Hit
TTCTTATAGATGAGGAGTGAGGGGGCCGATCCTGGGAATTGCTGGTACCA16370.15304314657457277No Hit
ATACCAGACCATTCAGACTGTCATCCCATGCAGCACAATAATAATCAGCC16260.15201475646319812No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC16170.1511733463720734No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT15800.1477142159974496No Hit
CCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGACACCTCTGCCTCCCTGGCCATCAGTGGGCTCCAGT15730.14705978592657482No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC15720.14696629591644986No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC15460.1445355556532007No Hit
GTCTGACACCTCTGCCTCCCTGGCCATCAGTGGGCTCCAGTCTGAGGATG15120.14135689530895176No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATAC15080.1409829352684519No Hit
TCCTCAGACTGGAGCCCACTGATGGCCAGGGAGGCAGAGGTGTCAGACTTGGAGCCAGAGAATCGGTCAGGGACC14980.1400480351672022No Hit
GAAGAAATACTGTCAATTGGTACCAGCAATTCCCAGGATCGGCCCCCTCA14970.13995454515707723No Hit
CTCCTCATCTATAAGAATGATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGACACCT14180.13256883435720476No Hit
GGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCA14130.13210138430657992No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT13940.13032507411420552No Hit
CCCTCAGCGTCTGGGGCCCCCGGGCAGAGGGTCACCATCTCTTGTTCTGG13670.1278008438408314No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT13520.12639849368895686No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG13470.12593104363833205No Hit
CTCTTGTTCTGGAACCAGCTCCAACATCAGAAGAAATACTGTCAATTGGTACCAGCAATTCCCAGGATCGGCCCC13340.12471567350670744No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA13260.12396775342570769No Hit
GTATTTCTTCTGATGTTGGAGCTGGTTCCAGAACAAGAGATGGTGACCCT13130.1227523832940831No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG13100.12247191326370821No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA13000.12153701316245853No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAG12900.12060211306120884No Hit
GGCCATCAGTGGGCTCCAGTCTGAGGATGAGGCTGATTATTATTGTGCTGCATGGGATGACAGTCTGAATGGT12880.12041513304095891No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC12880.12041513304095891No Hit
TTATTATTGTGCTGCATGGGATGACAGTCTGAATGGTCTGGTATTCGGCG12740.11910627289920936No Hit
GGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGT12590.11770392274733482No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG12500.11686251265621013No Hit
GATGAGGAGTGAGGGGGCCGATCCTGGGAATTGCTGGTACCAATTGACAG12170.11377734232208617No Hit
GCATGGGATGACAGTCTGAATGGTCTGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGG12050.11265546220058656No Hit
CTGATGGCCAGGGAGGCAGAGGTGTCAGACTTGGAGCCAGAGAATCGGTC11830.11059868197783726No Hit
GCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGC11820.1105051919677123No Hit
TTATTGTGCTGCATGGGATGACAGTCTGAATGGTCTGGTATTCGGCGGAG11780.11013123192721243No Hit
GTGTCAGACTTGGAGCCAGAGAATCGGTCAGGGACCCCTGAGGGCCGCTG11750.10985076189683751No Hit
ACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGC11720.10957029186646262No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGC11700.10938331184621268No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC11400.10657861154246362No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC11340.10601767148171382No Hit
CTATAAGAATGATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGACACCTCTGCCTCC11300.10564371144121394No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTC11300.10564371144121394No Hit
AGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGT11050.10330646118808975No Hit
GAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTC10890.10181062102609026No Hit
GCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCA10860.10153015099571534No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG10820.10115619095521547No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)