FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004689786

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689786
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1069633
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG61370.5737481921369293No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC48860.45679218947059413No Hit
GTCCTGGGCCCAGTCTGTACTGACTCAGCCACCCTCAGCGTCTGGGGCCC42490.39723905302098944No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG39560.3698464800543738No Hit
GTACTGACTCAGCCACCCTCAGCGTCTGGGGCCCCCGGGCAGAGGGTCAC34360.32123167478939035No Hit
AGTCTGTACTGACTCAGCCACCCTCAGCGTCTGGGGCCCCCGGGCAGAGG30600.2860794309824024No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCAG30550.28561198093177753No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTACTGACTCAGCCACCCTCAGCGTCT29840.2789741902129048No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT29380.27467364974715625No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC29280.27373874964590655No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG28400.26551162875490936No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG27170.2540123575095383No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA25520.23858650583891858No Hit
GGATGACAGTCTGAATGGTCTGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCC25090.23456643540354497No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT25030.23400549534279513No Hit
GATTATTATTGTGCTGCATGGGATGACAGTCTGAATGGTCTGGTATTCGG24850.23232267516054575No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG24470.22877005477579693No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC23810.22259971410754903No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG23720.22175830401642435No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC23100.2159619233886763No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC22480.21016554276092828No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT22290.20838923256855388No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA21650.20240587192055592No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG21050.19679647131305786No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG20980.19614204124218307No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCAGACCATTCAGA20920.19558110118143324No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG19990.18688653023981122No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT19930.18632559017906142No Hit
GTCTGAGGATGAGGCTGATTATTATTGTGCTGCATGGGATGACAGTCTGAATGGTCTGGTATTCGGCGGAGGGAC19100.17856591933868907No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATAC18170.16987134839706702No Hit
GTCTGACACCTCTGCCTCCCTGGCCATCAGTGGGCTCCAGTCTGAGGATG18160.16977785838694207No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA18030.16856248825531747No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTACTGACTCAGCCAC18020.1684689982451925No Hit
GTCTGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCC17760.16603825798194333No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA17560.16416845777944397No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC17480.16342053769844422No Hit
CCCCTGAGGGCCGCTGATCATTCTTATAGATGAGGAGTGAGGGGGCCGATCCTGGGAATTGCTGGTACCAATTGA17120.16005489733394537No Hit
CTCCTCATCTATAAGAATGATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGACACCT16960.15855905717194588No Hit
CCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGACACCTCTGCCTCCCTGGCCATCAGTGGGCTCCAGT16620.15538039682769697No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC16250.15192126645307313No Hit
CTCTTGTTCTGGAACCAGCTCCAACATCAGAAGAAATACTGTCAATTGGTACCAGCAATTCCCAGGATCGGCCCC15930.14892958612907417No Hit
GGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCA15730.14705978592657482No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC15650.14631186584557507No Hit
TTCTTATAGATGAGGAGTGAGGGGGCCGATCCTGGGAATTGCTGGTACCA15580.1456574357747003No Hit
GAAGAAATACTGTCAATTGGTACCAGCAATTCCCAGGATCGGCCCCCTCA15300.1430397154912012No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT15180.14191783536970157No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC15060.14079595524820196No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA14790.13827172497482781No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT14780.13817823496470283No Hit
CCCTCAGCGTCTGGGGCCCCCGGGCAGAGGGTCACCATCTCTTGTTCTGG14630.13677588481282832No Hit
GGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGT14440.13499957462045392No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAG14360.13425165453945417No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC14080.13163393425595507No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCAGACCATTCAGAC13940.13032507411420552No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA13730.1283617839015812No Hit
CTATAAGAATGATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGACACCTCTGCCTCC13610.1272399037800816No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC13600.1271464137699566No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT13320.12452869348645751No Hit
GATGAGGAGTGAGGGGGCCGATCCTGGGAATTGCTGGTACCAATTGACAG13210.12350030337508286No Hit
GGCCATCAGTGGGCTCCAGTCTGAGGATGAGGCTGATTATTATTGTGCTGCATGGGATGACAGTCTGAATGGT13140.12284587330420808No Hit
ACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGC12940.12097607310170871No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG12390.11583412254483547No Hit
TTATTATTGTGCTGCATGGGATGACAGTCTGAATGGTCTGGTATTCGGCG12360.11555365251446058No Hit
GTGTCAGACTTGGAGCCAGAGAATCGGTCAGGGACCCCTGAGGGCCGCTG12250.11452526240308591No Hit
GTATTTCTTCTGATGTTGGAGCTGGTTCCAGAACAAGAGATGGTGACCCT12240.11443177239296096No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG12190.11396432234233611No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA12130.11340338228158629No Hit
GAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTC12120.11330989227146133No Hit
GCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCA11910.11134660205883701No Hit
ATACCAGACCATTCAGACTGTCATCCCATGCAGCACAATAATAATCAGCC11780.11013123192721243No Hit
GCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGC11640.10882237178546288No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC11430.10685908157283854No Hit
GGGTCACCATCTCTTGTTCTGGAACCAGCTCCAACATCAGAAGAAATACT11430.10685908157283854No Hit
CTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTC11410.1066721015525886No Hit
CCTCTGCCTCCCTGGCCATCAGTGGGCTCCAGTCTGAGGATGAGGCTGAT11410.1066721015525886No Hit
GCATGGGATGACAGTCTGAATGGTCTGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGG11230.10498928137033918No Hit
TTATTGTGCTGCATGGGATGACAGTCTGAATGGTCTGGTATTCGGCGGAG11180.10452183131971432No Hit
GGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAAC11080.10358693121846464No Hit
CTGATGGCCAGGGAGGCAGAGGTGTCAGACTTGGAGCCAGAGAATCGGTC10970.10255854110709No Hit
AGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGT10920.10209109105646516No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGC10870.10162364100584033No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG10720.10022129085396579No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)