FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004689787

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689787
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences817074
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGGTAGTTCACCCCTCACCTTCGGCCAAGGGACACGACTACAAATTAAACGAACTGTGGCTGCACCAT21080.257993768006325No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT20340.24893706078029654No Hit
GTAATATAAGGCCGTGTCCTCGGCTCTCAGGCTGCTCATTTGCAGATACA19810.24245050019949232No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT19200.23498483613479318No Hit
GCCTTATATTACTGTGCGAAAGATTTAATCTCGTACGTGAGAGACTACTACTTTGACTCCTGGGGCCAGGGAACC18550.22702962032814653No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG17970.219931120069908No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA17900.2190744045214999No Hit
GTATTACTGTCAGCAGTATGGTAGTTCACCCCTCACCTTCGGCCAAGGGA17710.21674903374724933No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC16470.20157292974687727No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT16070.19667741232740238No Hit
CAGTAATATAAGGCCGTGTCCTCGGCTCTCAGGCTGCTCATTTGCAGATA14840.18162369626251723No Hit
ATATAAGGCCGTGTCCTCGGCTCTCAGGCTGCTCATTTGCAGATACAACG14520.17770728232693733No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC14180.1735460925203837No Hit
CTACCATACTGCTGACAGTAATACACTACAAAATCTTCAGGCTCCAGTCTGGTGATGGTGAGAGTGAAGTCTGTC14160.17330131664940995No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG13870.1697520665202907No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA13770.16852818716542198No Hit
CTCCTGTGCAGCCTCTGGATTCAGTTTTAGCAGCCGTGCCATGAGTTGGGTCCGCCAGGCTCCAGGGAGGGGG13430.16436699735886837No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG13220.16179685071364405No Hit
GTTCAAGGACCACCTGACCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG13190.16142968690718343No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG13140.16081774722974906No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC13050.15971625581036722No Hit
GTCTCCAGACACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGCCAGACTGTTAGCAG12680.15518790219735298No Hit
ATATTACTGTGCGAAAGATTTAATCTCGTACGTGAGAGACTACTACTTTGACTCCTGGGGCCAGGGAACCCTCGT12500.1529849193585893No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC12390.15163865206823374No Hit
CTCCTGCAGGGCCAGCCAGACTGTTAGCAGCGACTACTTAGCCTGGTACC12220.1495580571649569No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT12170.14894611748752254No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC12160.14882372955203568No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC12110.14821178987460132No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGT11910.1457640311648639No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC11860.14515209148742955No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG11780.14417298800353456No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG11660.1427043327776921No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG11430.13988941026149407No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA11370.13915508264857282No Hit
CATCTATGGTGCGTTCAGGAGGGCCACTGGCATCCCAAGCAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCA11300.13829836710016474No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT11250.13768642742273038No Hit
CAGTAATACACTACAAAATCTTCAGGCTCCAGTCTGGTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA11250.13768642742273038No Hit
GTCTGGGACAGACTTCACTCTCACCATCACCAGACTGGAGCCTGAAGATT10930.13377001348715048No Hit
CCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACGAGGGTTCCCTGGCCCCAGGAGTCAAAGTAGTAGTCT10880.13315807380971612No Hit
CTACGCAGACTCCGTGAAGGGCCGGTTCACCATCTCCAGAGACAATTCCAAGAACACGTTGTATCTGCAAATGAG10700.13095509097095245No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT10610.1298535995515706No Hit
GAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTTCTTGT10160.1243461424546614No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT10140.12410136658368766No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC10050.1229998751643058No Hit
GTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGT9650.11810435774483095No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC9630.11785958187385721No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG9510.11639092664801474No Hit
GATCAGGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGC9420.1152894352286329No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT9380.11479988348668542No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT9100.11137302129305303No Hit
GCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCAGTTTTAGCAGCCGTGCCATGAGTTGGGTCCG8990.11002675400269743No Hit
GTCACGGACTTGCCGGCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCT8890.10880287464782872No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG8870.10855809877685497No Hit
GTCCCAGACCCACTGCCACTGAACCTGCTTGGGATGCCAGTGGCCCTCCT8810.10782377116393374No Hit
GAGTTTGGGCTGAGCTGGCTTTTTCTTGTGGCTATTTTAAAAGGTGTCCAGTGTGCGGTGCAGCTGTTGGAGT8750.1070894435510125No Hit
GTGCAGCTGTTGGAGTCTGGGGGAGGCTTGGTACAGCCTGGGGGGTCCCT8660.10598795213163066No Hit
CAGTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCC8650.10586556419614379No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC8610.10537601245419631No Hit
TTATATTACTGTGCGAAAGATTTAATCTCGTACGTGAGAGACTACTACTT8590.10513123658322256No Hit
GTGCTGCAGAGGCTCAGCGGGAAGACCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACGAGGGTTCCCTGG8510.10415213309932761No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT8500.10402974516384074No Hit
CCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGGCTAAGTAGTCGCTGCTAACAGTCTGG8500.10402974516384074No Hit
GTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATGGG8490.10390735722835387No Hit
GTGTATTACTGTCAGCAGTATGGTAGTTCACCCCTCACCTTCGGCCAAGGGACACGACTACAAATTAAACGAA8430.10317302961543263No Hit
CTGCAGCACCCAGCCAGATGGGAACGTGGTCATCGCCTGCCTGGTCCAGG8360.10231631406702453No Hit
GTGTAGTGCTTCACGTGGCATGTCACGGACTTGCCGGCTAGGCACTGTGT8200.10035810709923458No Hit
CATTGACATGGGTGGGTTTACCCGCCAAGCGGTCGATGGTCTTCTGTGTGAAGGCCAGCGGCAGGGCCTCGTGGC8180.10011333122826084No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)