FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004689788

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689788
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences817074
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG44400.5434024335617093No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT21350.26129824226447057No Hit
GTATGGTAGTTCACCCCTCACCTTCGGCCAAGGGACACGACTACAAATTAAACGAACTGTGGCTGCACCAT20990.25689227658694314No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT18540.22690723239265964No Hit
GCCTTATATTACTGTGCGAAAGATTTAATCTCGTACGTGAGAGACTACTACTTTGACTCCTGGGGCCAGGGAACC18520.2266624565216859No Hit
GTAATATAAGGCCGTGTCCTCGGCTCTCAGGCTGCTCATTTGCAGATACA16700.20438785226307532No Hit
GTATTACTGTCAGCAGTATGGTAGTTCACCCCTCACCTTCGGCCAAGGGA15810.19349532600474376No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC15240.18651921368199206No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT15070.18443861877871529No Hit
CTCCTGTGCAGCCTCTGGATTCAGTTTTAGCAGCCGTGCCATGAGTTGGGTCCGCCAGGCTCCAGGGAGGGGG15010.18370429116579404No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC14150.1731789287139231No Hit
GTTCAAGGACCACCTGACCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG14140.1730565407784362No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG13690.16754908368152702No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC13670.16730430781055325No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA13600.16644759226214514No Hit
ATATAAGGCCGTGTCCTCGGCTCTCAGGCTGCTCATTTGCAGATACAACG13480.16497893703630273No Hit
CAGTAATATAAGGCCGTGTCCTCGGCTCTCAGGCTGCTCATTTGCAGATA13260.16228640245559153No Hit
CTACCATACTGCTGACAGTAATACACTACAAAATCTTCAGGCTCCAGTCTGGTGATGGTGAGAGTGAAGTCTGTC13160.16106252310072283No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA12980.15885954026195914No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC12960.1586147643909854No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC12870.15751327297160356No Hit
GTCTCCAGACACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGCCAGACTGTTAGCAG12620.15445357458443176No Hit
ATATTACTGTGCGAAAGATTTAATCTCGTACGTGAGAGACTACTACTTTGACTCCTGGGGCCAGGGAACCCTCGT12500.1529849193585893No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG12290.150414772713365No Hit
GTCTGGGACAGACTTCACTCTCACCATCACCAGACTGGAGCCTGAAGATT11720.14343866039061334No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA11710.14331627245512646No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG11640.14245955690671835No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG11210.1371968756807829No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT11120.13609538426140105No Hit
CTCCTGCAGGGCCAGCCAGACTGTTAGCAGCGACTACTTAGCCTGGTACC10970.13425956522909796No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC10750.13156703064838682No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC10380.12703867703537255No Hit
CAGTAATACACTACAAAATCTTCAGGCTCCAGTCTGGTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA10380.12703867703537255No Hit
CATCTATGGTGCGTTCAGGAGGGCCACTGGCATCCCAAGCAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCA10360.12679390116439881No Hit
CCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACGAGGGTTCCCTGGCCCCAGGAGTCAAAGTAGTAGTCT10180.12459091832563514No Hit
CTACGCAGACTCCGTGAAGGGCCGGTTCACCATCTCCAGAGACAATTCCAAGAACACGTTGTATCTGCAAATGAG9600.11749241806739659No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG9570.11712525426093598No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC9560.11700286632544911No Hit
CTGCAGCACCCAGCCAGATGGGAACGTGGTCATCGCCTGCCTGGTCCAGG9320.11406555587376419No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT9260.11333122826084295No Hit
GTGCAGCTGTTGGAGTCTGGGGGAGGCTTGGTACAGCCTGGGGGGTCCCT9260.11333122826084295No Hit
GAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTTCTTGT9230.11296406445438234No Hit
CCTGTACACCACGAGCAGCCAGCTGACCCTGCCGGCCACACAGTGCCTAG9190.11247451271243485No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG9140.1118625730350005No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC9090.11125063335756616No Hit
GAGTTTGGGCTGAGCTGGCTTTTTCTTGTGGCTATTTTAAAAGGTGTCCAGTGTGCGGTGCAGCTGTTGGAGT9070.1110058574865924No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT9060.11088346955110553No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC8840.10819093497039436No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG8810.10782377116393374No Hit
GATCAGGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGC8700.10647750387357816No Hit
GTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGT8570.10488646071224884No Hit
GGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGACCTGTACACCACGAGCAGCCAGCTGAC8510.10415213309932761No Hit
GTCCCAGACCCACTGCCACTGAACCTGCTTGGGATGCCAGTGGCCCTCCT8410.1029282537444589No Hit
GTGCTGCAGAGGCTCAGCGGGAAGACCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACGAGGGTTCCCTGG8400.10280586580897202No Hit
GTGTATTACTGTCAGCAGTATGGTAGTTCACCCCTCACCTTCGGCCAAGGGACACGACTACAAATTAAACGAA8390.10268347787348514No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT8390.10268347787348514No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC8370.1024387020025114No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG8330.10194915026056392No Hit
GCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCAGTTTTAGCAGCCGTGCCATGAGTTGGGTCCG8330.10194915026056392No Hit
CCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGGCTAAGTAGTCGCTGCTAACAGTCTGG8240.10084765884118208No Hit
GTGCAGCCACAGTTCGTTTAATTTGTAGTCGTGTCCCTTGGCCGAAGGTG8220.10060288297020832No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)