FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004689789

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689789
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1366113
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC49980.3658555331806373No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAGTGGT48660.35619308212424594No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT40460.296168764955754No Hit
GATTATTACTGCTGCTCATATGCAGGTAATACCACTTTCGGCGGAGGGAC38570.2823338918522845No Hit
GTAATACACGGCCGTGTCCGCGGCAGTCACAGAGGTCAGGTTCAGGGAGA37740.27625825974864454No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG36830.26959702455067774No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC35580.2604469762018223No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAGTGGTATTACCTGCATATGAGCAGCAGTAATAATCAGCCT33400.2444892918814183No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTG32950.24119527447583033No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT32460.237608455523079No Hit
GGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC30070.22011356308006733No Hit
GTTATAACCTTGTCTCCTGGTACCAACAACACCCAGGCAAAGCCCCCAAA29950.2192351584385772No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGTAGTGATGTT28390.20781589809920556No Hit
GTAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGAGATTGTCAGGGAGG27430.20078866096728457No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT27120.19851944897676838No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG25430.1861485836091158No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG25350.18556298051478903No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC25080.18358657007143625No Hit
GACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCT24270.1776573387413779No Hit
GTCGATCACCATCTCCTGCACTGGAACCAGTAGTGATGTTGGGAGTTATAACCTTGTCTCCTGGTACCAACAACA23870.17472932326974416No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT23750.17385091862825403No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG23500.17202090895848293No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC23440.17158170663773786No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC23430.17150850625094705No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG23030.16858049077931328No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA22810.16697008226991472No Hit
CTCCTGCACTGGAACCAGTAGTGATGTTGGGAGTTATAACCTTGTCTCCTGGTACCAACAACACCCAGGCAAAGC22250.16287086060962747No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC21810.15965004359083035No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG21800.1595768432040395No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT21480.1572344308267325No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA21120.1545992169022621No Hit
CCCCTGAGGGCCGCTTACTGACCTCATAAATCATGAGTTTGGGGGCTTTGCCTGGGTGTTGTTGGTACCAGGAGA20950.15335481032681778No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG20330.14881638634578545No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA20210.14793798170429534No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA20180.14771838054392278No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG19890.14559556932698833No Hit
GGTTATAACTCCCAACATCACTACTGGTTCCAGTGCAGGAGATGGTGATC19460.14244795269498203No Hit
GGCCTGGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC19310.1413499468931194No Hit
CAGTAATACACGGCCGTGTCCGCGGCAGTCACAGAGGTCAGGTTCAGGGAGAACTGATTCTTAGACGTGTCTA19130.1400323399308842No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC18870.13812912987432227No Hit
GGTAATACCACTTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACT18520.13556711633664273No Hit
CTGCTGCTCCTCACCCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTC18520.13556711633664273No Hit
CTTATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGT18070.13227309893105477No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT17570.12861307959151255No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT17230.12612426644062386No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC17100.1251726614123429No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT16940.1240014552236894No Hit
GCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC16790.12290344942182674No Hit
GTGTCTGGTGGCTCCATCAGCAGTGGTGGTTACTACTGGAACTGGATCCG16610.12158584245959154No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC16570.12129304091242818No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGC16440.1203414358841472No Hit
CATTTACCCGGAGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG16370.11982903317661131No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG16240.11887742814833033No Hit
CCTCAGGGGTTTCTAATCGCTTCTCTGGCTCCAAGTCTGGCAACACGGCCTCCCTGACAATCTCTGGGCTCCAGG15760.11536380958236984No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAGTGGTATTACCTGCAT15320.11214299256357271No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC15250.1116305898560368No Hit
GCTTGGTCCCTCCGCCGAAAGTGGTATTACCTGCATATGAGCAGCAGTAA15040.11009338173342909No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAGTGGTATTACCTG15020.10994698095984738No Hit
TCTCTGGGCTCCAGGCTGAGGACGAGGCTGATTATTACTGCTGCTCATAT14880.10892217554477558No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA14840.10862937399761219No Hit
AGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCG14740.10789737012970375No Hit
GCCTGGAGCCCAGAGATTGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCC14730.10782416974291292No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACACCTGTGGTTCTTCCTCCTGCTGGTGGCAGCTCCCAGATGGGTC14680.10745816780895871No Hit
CAAGAGGCAGCGCTCTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTGC14570.1066529635542594No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC13890.10167533725248204No Hit
GGTATTACCTGCATATGAGCAGCAGTAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGAGATTGTCAGGGAGGC13700.10028452990345602No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATACCG200.0077456851.084555
CGTATGC4150.048.24310341
TCGTATG4050.047.6430540
TAGTGCC305.7668285E-446.85059753
ATGCCGT4350.046.2256744
CCCTAAG5650.045.8707728
ATCTCGT3450.045.75851437
CCGTCTT4450.045.3796447
CTTATAC5700.044.825771
TGCCGTC4650.044.7912645
CACGAGA5800.044.6304821
GCCGTCT4450.044.5239346
TAAGACA5700.044.3563931
CCCACGA5950.044.07455419
ACGAGAC5800.044.04646322
GTATGCC4750.043.6642142
TATACAC5950.043.5005653
ATCTCCG5900.043.2919910
TCTCCGA6050.042.7815511
TTATACA6200.042.2958072
Produced by FastQC (version 0.11.5)