FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004689796

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004689796
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences821451
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG96381.1732897032202771No Hit
GGATTATAGTGTTGGCAGTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTC40220.48962141381530977No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT39870.48536066058718047No Hit
CTATAATCCTTATTCGACGTTCGGTCAAGGGACCAAGGTGGAAGTCAAACGAACTGTGGCTGCACCATCTGTCTT32860.40002386021807756No Hit
GAGTATTAGTACCTGGTTGGCCTGGTATCAGCAAAAACCAGGGAAAGCCCCTAAGGTCCTGATCTATAAGGCGT29700.3615553453583963No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC29700.3615553453583963No Hit
GTATTAGTACCTGGTTGGCCTGGTATCAGCAAAAACCAGGGAAAGCCCCT27960.34037331502426804No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC27550.3353821469570309No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC27200.33112139372890165No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT23170.2820618637021563No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC22430.2730534140198259No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG21320.2595407394963303No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC20480.24931493174882008No Hit
GAATTCACTCTCACCATCAGCAGCCTGCAGCCTGATGATTTTGCAACTTA18630.22679380754299402No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT18230.2219243752822749No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA17930.21827230108673554No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT17290.2104812094695849No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG17180.20914211559788717No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG17080.20792475753270734No Hit
CCTTCCACCCTGTCTGCATCTATAGGGGACAGAGTCACCATCACTTGCCG16990.20682913527404556No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG16600.2020814388198444No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC16220.19745547817216122No Hit
GTACTAATACTCTGACTGGCCCGGCAAGTGATGGTGACTCTGTCCCCTATAGATGCAGACAGGGTGGAAGGAGA16210.19733374236564324No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG15930.19392513978313983No Hit
GTCTGCATCTATAGGGGACAGAGTCACCATCACTTGCCGGGCCAGTCAGA15860.193072989137514No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT15840.19282951752447802No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAGTGTCAGAT15790.19222083849188815No Hit
GTCCTGATCTATAAGGCGTCTAGTTTAGAAAGTGGGGTCCCATCAAGGTT15400.18747314203768697No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA15300.1862557839725072No Hit
ATAGGGGACAGAGTCACCATCACTTGCCGGGCCAGTCAGAGTATTAGTACCTGGTTGGCCTGGTATCAGCAAAAA15180.18479495429429144No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT14920.181629823324824No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAGTGT14690.17882989977491048No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG14500.1765169194510689No Hit
GTCAAGGGACCAAGGTGGAAGTCAAACGAACTGTGGCTGCACCATCTGTC14350.17469088235329921No Hit
GATCTATAAGGCGTCTAGTTTAGAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCA13920.16945624267302614No Hit
GAGTGAATTCTGTCCCAGATCCACTGCCGCTGAACCTTGATGGGACCCCA13700.1667780549296306No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT13650.16616937589704073No Hit
CCTTAGGGGCTTTCCCTGGTTTTTGCTGATACCAGGCCAACCAGGTACTA13240.1611782078298036No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG12940.15752613363426424No Hit
CTAATACTCTGACTGGCCCGGCAAGTGATGGTGACTCTGTCCCCTATAGA12680.1543610026647968No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG11980.1458394962085383No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG11870.14450040233684056No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA11820.14389172330425065No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAGTGTCAGATCCAGATGACCCAGTCTCCTTCCACC11700.1424308936260349No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG11600.1412135355608551No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA11570.1408483281413012No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC11180.13610063168710002No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGACTTCCACCTTGGTCCCTTGACCGAACGTCGAATAAGGATT10970.13354417975022248No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT10770.1311094636198629No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA10630.1294051623286112No Hit
CCCCTAAGGTCCTGATCTATAAGGCGTCTAGTTTAGAAAGTGGGGTCCCA10310.1255096165200359No Hit
GTGTCAGATCCAGATGACCCAGTCTCCTTCCACCCTGTCTGCATCTATAG10310.1255096165200359No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA10230.12453573006789206No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT10230.12453573006789206No Hit
TCTATAAGGCGTCTAGTTTAGAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACT10200.12417052264833811No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA9990.12161407071146058No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC9870.12015324103324483No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC9740.1185706755485111No Hit
GTCTCCTTCCACCCTGTCTGCATCTATAGGGGACAGAGTCACCATCACTTGCCGGGCCAGTCAGAGTATTAGTAC9700.1180837323224392No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC9690.11796199651592121No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT9680.11784026070940325No Hit
GATCAGGACCTTAGGGGCTTTCCCTGGTTTTTGCTGATACCAGGCCAACC9670.11771852490288526No Hit
ACTTATTACTGCCAACACTATAATCCTTATTCGACGTTCGGTCAAGGGAC9620.11710984587029537No Hit
CCTTATAGATCAGGACCTTAGGGGCTTTCCCTGGTTTTTGCTGATACCAGGCCAACCAGGTACTAATACTCTGA9530.11601422361163355No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC9470.1152838087725257No Hit
GGGTGGAAGGAGACTGGGTCATCTGGATCTGACACTTGGCACCTGGGAGCCAGAGCAGCAGGAGCCCCAGGAG9290.11309256425520207No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC9290.11309256425520207No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC9260.11272735683564815No Hit
GAGTCACCATCACTTGCCGGGCCAGTCAGAGTATTAGTACCTGGTTGGCCTGGTATCAGCAAAAACCAGGGAAAG9240.11248388522261218No Hit
CACCATCAGCAGCCTGCAGCCTGATGATTTTGCAACTTATTACTGCCAAC9110.11090131973787846No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT9050.11017090489877059No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC9050.11017090489877059No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC9010.10968396167269868No Hit
GTGCAGCCACAGTTCGTTTGACTTCCACCTTGGTCCCTTGACCGAACGTCGAATAAGGATTATAGTGTTGGCAGT8820.1073709813488571No Hit
CATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACTCTCACCATCAGCAGCCTGCAGCCTGATGATTTTG8810.1072492455423391No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA8750.10651883070323122No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAG8730.10627535909019528No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC8620.10493626521849751No Hit
ACCTTGATGGGACCCCACTTTCTAAACTAGACGCCTTATAGATCAGGACCTTAGGGGCTTTCCCTGGTTTTTG8540.10396237876635367No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCC8490.10335369973376379No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC8460.10298849231420985No Hit
TTATAGATCAGGACCTTAGGGGCTTTCCCTGGTTTTTGCTGATACCAGGC8370.10189287005554805No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGT8360.10177113424903007No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT8290.10091898360340423No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)