FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004691312

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691312
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39350360
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATATCTCGTATG1024090.26024920737701TruSeq Adapter, Index 7 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA147400.039.8818971
ATCGGAA148350.039.705142
CTCGTAT121600.039.12438244
TCTCGTA121950.039.03089543
TCGTATG122550.038.93127445
ACACGTC152150.038.44467513
ACGAGTG130650.037.93642832
TATCTCG125800.037.8858241
CGTCTGA153800.037.85679216
GTCACGA131700.037.45985429
CACGAGT132350.037.3641531
TCGGAAG158550.037.248893
AGTCACG137100.036.14923528
ACGTCTG162250.036.06546815
CGGAAGA164000.036.0247654
ATCTCGT132300.035.9598142
CACGTCT166850.035.084514
CACACGT173700.033.7657212
GCACACG176950.033.31083711
CGAGTGG149150.033.20076833
Produced by FastQC (version 0.11.5)