FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004691313

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691313
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40155644
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGATATATCTCGTATG1352970.3369314659727534TruSeq Adapter, Index 25 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA150750.042.5967031
CTCGTAT156000.040.99983644
ATCGGAA157500.040.7807662
TCTCGTA157450.040.65083343
TATCTCG157350.040.6051841
ACACGTC163400.039.96914313
TCGTATG161100.039.7996445
TCGGAAG162750.039.5343743
CGTCTGA165100.039.53033416
ATCTCGT165500.038.76870742
CGGAAGA169450.037.957924
ACGTCTG173700.037.6508715
CACGTCT177950.036.77693614
GCACACG186300.035.22519711
CACACGT193150.034.0574812
GTCACAC227650.028.68887529
AGAGCAC247800.026.7643098
AGCACAC254300.026.09789710
ACTGATA252600.025.91754234
GAGCACA256900.025.886319
Produced by FastQC (version 0.11.5)