FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004691314

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691314
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22105219
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCC458560.20744422391834252TruSeq Adapter, Index 2 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA50400.040.5449681
CTCGTAT51050.038.96802542
CGATGTA52150.038.83403834
ACCGATG52550.038.49440432
TCTCGTA51550.038.45910341
TATCTCG51950.038.2496139
CGTATGC52500.038.0203644
ATCGGAA54300.037.8681832
CACCGAT53800.037.51636531
CCGATGT54050.037.5105533
TCGTATG53850.037.2343643
ATCTCGT55000.036.16941540
ACACGTC57150.035.7428213
TCGGAAG57800.035.5751273
TCACCGA58650.034.41313630
CGTCTGA59800.033.9709616
GTCACCG60150.033.59235829
CGGAAGA63350.032.600494
ACGTCTG64400.031.57939115
CACGTCT65700.031.02287714
Produced by FastQC (version 0.11.5)