FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004691315

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691315
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17212324
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGCC342340.1988923750215253TruSeq Adapter, Index 3 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT40200.038.84684842
TCGTATG40450.038.6621643
CGTATGC41100.038.21429444
TCTCGTA41350.037.60331341
ATCGGAA49300.037.434112
GATCGGA50150.036.6447221
ACACGTC50500.034.92526613
CGTCTGA51450.034.1492216
TCGGAAG55350.033.338813
ACGTCTG55150.032.18448615
ATCTCGT48750.032.03380240
CACGTCT58600.030.28966314
GTATGCC53100.029.74779745
CACACGT60750.029.40284212
CGGAAGA63950.028.9961624
CATCTCG57600.027.3072739
GCACACG66850.026.75351111
CTTAGGC63600.024.94383233
ACTTAGG64800.024.65539632
TAGGCAT72350.022.23821635
Produced by FastQC (version 0.11.5)