FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004691317

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691317
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21670511
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATATCTCGTATG3857061.7798657355149585TruSeq Adapter, Index 7 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA409750.044.253761
ATCGGAA413650.043.9095542
ACACGTC416000.043.7101813
TCGGAAG415600.043.6981163
GTCACGA413050.043.6410729
CTCGTAT410900.043.6254444
CGTCTGA416850.043.59946416
TCTCGTA411150.043.59850743
TCGTATG413100.043.56739845
TATCTCG412650.043.39570641
CACGAGT416700.043.307531
ACGAGTG417100.043.2821532
AGTCACG416650.043.26939428
CGGAAGA421650.043.231194
ACGTCTG421500.043.1238115
ATCTCGT416050.043.06910742
CACGTCT422400.043.04790514
GCACACG427500.042.6238211
CACACGT430900.042.29794312
CGAGTGG430400.042.00216733
Produced by FastQC (version 0.11.5)