FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004691319

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691319
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17842598
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTATG193230.10829700921356855TruSeq Adapter, Index 13 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA24850.037.628971
CTCGTAT25150.035.96970444
TCTCGTA25950.034.8609143
TCGTATG26300.034.48138845
ATCGGAA27450.033.8482322
TCGGAAG28600.032.6445273
CGTCTGA29150.031.56508816
ACACGTC29750.031.15533813
ATCTCGT29700.030.76235842
CGGAAGA32650.028.5952054
ACGTCTG32750.028.30141415
CACGTCT35700.026.34088114
AATCTCG36950.025.1527641
GCACACG39650.023.43299911
CACACGT40300.023.1667612
AGTCAAC50300.018.11789934
GTCAACA58950.015.68840635
CAGTCAA61800.015.43825533
AGAGCAC66200.014.7489158
CCAGTCA70200.013.81338526
Produced by FastQC (version 0.11.5)