FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004691322

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691322
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19377610
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCACATAATCTCGTATGC402951720.79470584865729TruSeq Adapter, Index 6 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCACATAATCTCGTATG381792619.70277036228926TruSeq Adapter, Index 6 (97% over 37bp)
AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTT971590.5013982632533115Illumina Single End PCR Primer 1 (100% over 51bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACGCCACATAATCTCGTATGCCG855650.4415663231946561TruSeq Adapter, Index 6 (97% over 34bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCACATAAACTCGTATGC696820.35960059057850785TruSeq Adapter, Index 6 (97% over 36bp)
TCGGAAGAGCACACGTCTGAACTCCAGTCACGCCACATAATCTCGTATGCC691880.3570512565791137TruSeq Adapter, Index 6 (97% over 35bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCACATAACCTCGTATGC552150.2849422606812708TruSeq Adapter, Index 6 (97% over 36bp)
GAAGAGCACACGTCTGAACTCCAGTCACGCCACATAATCTCGTATGCCGTC403920.208446758914025TruSeq Adapter, Index 6 (96% over 32bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCACATAACCTCGTATG368030.18992538295486389TruSeq Adapter, Index 6 (97% over 37bp)
NATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCACATAATCTCGTATG329330.16995387976122958TruSeq Adapter, Index 6 (97% over 37bp)
NTCGGAAGAGCACACGTCTGAACTCCAGTCACGCCACATAATCTCGTATGC324240.16732713683472833TruSeq Adapter, Index 6 (97% over 36bp)
AATGATACGGCGACCACCGAGATCGGAAGAGCACACGTCTGAACTCCAGTC264370.1364306537287106Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCACATAAGCTCGTATGC262870.13565656445763952TruSeq Adapter, Index 6 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCACATAAGCTCGTATG236830.12221837471184528TruSeq Adapter, Index 6 (97% over 37bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)