FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004691325

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691325
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15290844
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATAGACAATCTCGTATG158015010.333961944808278TruSeq Adapter, Index 9 (97% over 36bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGATAGACAATCTCGTATGC12554158.210240062615249TruSeq Adapter, Index 9 (97% over 35bp)
AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTT1223500.8001520386971445Illumina Single End PCR Primer 1 (100% over 51bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACGATAGACAATCTCGTATGCCG499400.3266006768494924TruSeq Adapter, Index 9 (96% over 33bp)
GAAGAGCACACGTCTGAACTCCAGTCACGATAGACAATCTCGTATGCCGTC331240.2166263680409008TruSeq Adapter, Index 9 (96% over 31bp)
GAAGAGCACACGTCTGAACTCCAGTCACGATAGACAATCTCGTATGCCGTA287120.18777249967366091TruSeq Adapter, Index 9 (96% over 31bp)
TCGGAAGAGCACACGTCTGAACTCCAGTCACGATAGACAATCTCGTATGCC284670.18617023363785543TruSeq Adapter, Index 9 (97% over 34bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGATAGACAACCTCGTATGC193020.12623240417598924TruSeq Adapter, Index 9 (97% over 35bp)
AATGATACGGCGACCACCGAGATCGGAAGAGCACACGTCTGAACTCCAGTC158710.10379413981334189Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGATAGACAAACTCGTATGC157570.10304859561708955TruSeq Adapter, Index 9 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATACG246700.041.7752953
GATACGG246450.041.7253074
ATACGGC247050.041.53485
CGCCGTA13800.041.073648
TACGGCG249850.040.5471886
ACGGCGA249850.040.4571657
CGGCGAC251450.040.0834438
GGCGACC249200.039.8947689
GATCGGA2054850.039.7611921
GCGACCA249200.039.70522310
CGACCAC250050.039.45331611
GTCGCCG15050.039.306196
ATGATAC264250.038.6770132
TCGCCGT14800.038.602357
ACCACCG252750.038.4178113
CCACCGA253000.038.33539614
CACCGAG257100.037.78529715
ACCGAGA255850.037.66220516
CGTATGC1840700.037.5478845
GACCACC259300.037.52543312
Produced by FastQC (version 0.11.5)