FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004691326

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691326
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20766493
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGTCTAATCTCGTATGC1011913048.72816031093936TruSeq Adapter, Index 6 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGTCTAATCTCGTATG407139319.605587712860327TruSeq Adapter, Index 6 (97% over 36bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGTCTAACCTCGTATGC1229350.5919872941473556TruSeq Adapter, Index 6 (97% over 35bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGTCTAAACTCGTATGC1123040.5407942496597764TruSeq Adapter, Index 6 (97% over 35bp)
TCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGTCTAATCTCGTATGCC1019810.49108436364291264TruSeq Adapter, Index 6 (97% over 34bp)
NTCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGTCTAATCTCGTATGC744580.35854874484584376TruSeq Adapter, Index 3 (96% over 33bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGTCTAAGCTCGTATGC719070.34626453296664006TruSeq Adapter, Index 6 (97% over 35bp)
GAAGAGCACACGTCTGAACTCCAGTCACTCCGTCTAATCTCGTATGCCGTC600890.28935554982731077TruSeq Adapter, Index 6 (96% over 31bp)
AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTT528810.25464578925290854Illumina Single End PCR Primer 1 (100% over 51bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACTCCGTCTAATCTCGTATGCCG499300.24043539754160706TruSeq Adapter, Index 6 (96% over 33bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGTCTAATCTCGGTGGT480960.23160386301143868TruSeq Adapter, Index 6 (97% over 35bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGCTAATCTCGTATGC438320.21107078600127618TruSeq Adapter, Index 6 (97% over 35bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCCTAATCTCGTATGC433860.20892309548848714TruSeq Adapter, Index 6 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGTCTAACCTCGTATG431140.20761329320266064TruSeq Adapter, Index 6 (97% over 36bp)
ATGATACGGCGACCACCGAGATCATTGATCGGAAGAGCACACGTCTGAACT420400.20244150035347808Illumina Multiplexing PCR Primer 2.01 (96% over 26bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGTCCAATCTCGTATGC323170.15562088408476096TruSeq Adapter, Index 6 (97% over 35bp)
NATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGTCTAATCTCGTATG312070.15027573505068959TruSeq Adapter, Index 3 (97% over 34bp)
GAAGAGCACACGTCTGAACTCCAGTCACTCCGTCTAATCTCGTATGCCGTA311320.14991457633217126TruSeq Adapter, Index 6 (96% over 31bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGTCTACTCTCGTATGC255430.12300102862818484TruSeq Adapter, Index 6 (97% over 35bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGTCTAATATCGTATGC253310.121980153317173TruSeq Adapter, Index 6 (97% over 35bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTGGCTTCAATCTCGTATGC233850.11260928843401724TruSeq Adapter, Index 10 (97% over 38bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCCTCTAATCTCGTATGC228850.110201563643895TruSeq Adapter, Index 6 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGTCTAATCTCGGTGG228180.10987892852201862TruSeq Adapter, Index 6 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC12443900.042.3252445
CTTCGCG2150.041.8519932
GATCGGA5095250.041.3148961
CGCGCAA2600.039.80049535
CGGGTAA11250.039.7928135
CGCGTAA5700.039.4665535
CCGGGTA7850.039.26136434
CGTCCAA47400.039.2492335
GATCGAA18700.039.1903081
TTCGCGC1150.039.12251333
TCCGTCC46750.039.07230433
AATGATA137200.038.928721
GTCTCGT15250.038.79495641
CCGTCCA47400.038.77556634
CGTCTAG11200.038.76531235
CGCCCAA10600.038.62509535
CCAATCT105850.038.59314338
CGGGGTA5600.038.56538834
ACTCGCT10300.038.2197731
CACTCCC88500.038.1018130
Produced by FastQC (version 0.11.5)