FastQCFastQC Report
Tue 13 Sep 2022
EGAF00004691327

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691327
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20835230
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGAGAACAATCTCGTATG870977341.80310464535309TruSeq Adapter, Index 2 (97% over 36bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGGAGAACAATCTCGTATGC657138431.539771819173584TruSeq Adapter, Index 2 (97% over 35bp)
GAAGAGCACACGTCTGAACTCCAGTCACGGAGAACAATCTCGTATGCCGTC2138561.0264153551460675TruSeq Adapter, Index 2 (96% over 31bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGGAGAACAAACTCGTATGC1934870.928653055425834TruSeq Adapter, Index 2 (97% over 35bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACGGAGAACAATCTCGTATGCCG1247610.5987982854040969TruSeq Adapter, Index 2 (96% over 33bp)
AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTT1211430.5814334662972283Illumina Single End PCR Primer 1 (100% over 51bp)
TCGGAAGAGCACACGTCTGAACTCCAGTCACGGAGAACAATCTCGTATGCC1110780.5331258642213214TruSeq Adapter, Index 2 (97% over 34bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGAGAACAAGCTCGTATG1043950.5010503843730068TruSeq Adapter, Index 2 (97% over 36bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGGAGAACAACCTCGTATGC932950.4477752345426473TruSeq Adapter, Index 2 (97% over 35bp)
GAAGAGCACACGTCTGAACTCCAGTCACGGAGAACAATCTCGTATGCCGTA772330.37068465286920277TruSeq Adapter, Index 2 (96% over 31bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGGAGAACAAGCTCGTATGC748630.3593096884459639TruSeq Adapter, Index 2 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGAGAACAACCTCGTATG718940.34505978575710466TruSeq Adapter, Index 2 (97% over 36bp)
NATCGGAAGAGCACACGTCTGAACTCCAGTCACGGAGAACAATCTCGTATG665550.31943491864500656TruSeq Adapter, Index 11 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGAGAACAAACTCGTATG612410.29393004060910294TruSeq Adapter, Index 2 (97% over 36bp)
AATGATACGGCGACCACCGAGATCGGAAGAGCACACGTCTGAACTCCAGTC483330.23197728078835703Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
NTCGGAAGAGCACACGTCTGAACTCCAGTCACGGAGAACAATCTCGTATGC481390.23104616555708768TruSeq Adapter, Index 11 (97% over 34bp)
GATCGGAAGAGCACACATCTGAACTCCAGTCACGGAGAACAATCTCGTATG222650.1068622712588246TruSeq Adapter, Index 11 (97% over 35bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)