FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004691328

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691328
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8370866
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC6350.021.2600845
CTCGTAT6650.018.94712643
TCTCGTA6650.017.93124642
TCGTATG8100.015.83313244
ATCTCGT8050.014.53310841
ACACGTC10550.012.79329412
CGTCTGA11200.011.84997815
ACGTCTG12750.010.40939314
CACGTCT13700.09.52337813
CACACGT16900.08.25250911
ATCGGAA15800.08.1693061
TCGGAAG16150.08.0817932
GCACACG18000.07.748188510
CGGAAGA18250.07.39676863
AATCTCG16300.07.315333440
GATCGGA12300.06.81183341
CACACTA20700.06.7383630
ACTATGC22450.06.51369333
GTCACAC20950.06.4433728
CTATGCA26100.06.033684334
Produced by FastQC (version 0.11.5)