FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004691330

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691330
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18234871
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGAGATCATCTCGTATGC963526352.8397650852589TruSeq Adapter, Index 7 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGAGATCATCTCGTATG264371114.49810640283663TruSeq Adapter, Index 7 (97% over 37bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGAGATCAACTCGTATGC1267270.6949706416897602TruSeq Adapter, Index 7 (97% over 36bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGAGATCATCTCGGTGGT1260590.6913073308826807TruSeq Adapter, Index 7 (97% over 36bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGAGATCACCTCGTATGC1066670.5849616375130924TruSeq Adapter, Index 7 (97% over 36bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGAGATCAGCTCGTATGC870150.4771901046078143TruSeq Adapter, Index 7 (97% over 36bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACAAGAGATCATCTCGTATGCCG741870.40684137551617444TruSeq Adapter, Index 7 (97% over 34bp)
GGAAGAGCACACGTCTGAACTCCAGTCACAAGAGATCATCTCGTATGCCGT660690.36232227801337336TruSeq Adapter, Index 7 (96% over 33bp)
NTCGGAAGAGCACACGTCTGAACTCCAGTCACAAGAGATCATCTCGTATGC652230.357682815524168TruSeq Adapter, Index 1 (96% over 33bp)
TCGGAAGAGCACACGTCTGAACTCCAGTCACAAGAGATCATCTCGTATGCC564520.30958266718750027TruSeq Adapter, Index 7 (97% over 35bp)
ATCGAAGAGCACACGTCTGAACTCCAGTCACAAGAGATCATCTCGTATGCC473270.25954118348300903TruSeq Adapter, Index 1 (96% over 30bp)
GAAGAGCACACGTCTGAACTCCAGTCACAAGAGATCATCTCGTATGCCGTC406060.2226832314854325TruSeq Adapter, Index 7 (96% over 32bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGAGATCATCTCGGTGG388650.2131355905945263TruSeq Adapter, Index 7 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGAGATCACCTCGTATG365340.20035239075724748TruSeq Adapter, Index 7 (97% over 37bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGAGACCATCTCGTATGC328610.18020966531652458TruSeq Adapter, Index 7 (97% over 36bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGAGCTCATCTCGTATGC280350.1537438899348397TruSeq Adapter, Index 7 (97% over 36bp)
ATCGGAAAGCACACGTCTGAACTCCAGTCACAAGAGATCATCTCGTATGCC278410.15267999428128665TruSeq Adapter, Index 1 (96% over 27bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGAGAACATCTCGTATGC261680.14350526526894541TruSeq Adapter, Index 7 (97% over 36bp)
AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTT241760.1325811408262773Illumina Single End PCR Primer 1 (100% over 51bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGAGATCATCTCGGGATC235570.12918654593169318TruSeq Adapter, Index 7 (97% over 36bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGAGAGCATCTCGTATGC197560.10834186871955388TruSeq Adapter, Index 7 (97% over 36bp)
NATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGAGATCATCTCGTATG196030.10750281699278268TruSeq Adapter, Index 1 (97% over 34bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGCGATCATCTCGTATGC194900.10688312519457911TruSeq Adapter, Index 10 (97% over 36bp)
CTGAACTCCAGTCACAAGAGATCAGAGATCGGAAGAGCACACGTCTGAACT194530.10668021726065405Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)