FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004691332

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691332
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17009582
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCAATCTCGTATG3964192.330562855689223TruSeq Adapter, Index 9 (97% over 39bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCAGCAATCTCGTATGC1777581.0450462568686285TruSeq Adapter, Index 9 (97% over 38bp)
AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTT406780.23914755812341537Illumina Single End PCR Primer 1 (100% over 51bp)
GAAGAGCACACGTCTGAACTCCAGTCACTATCAGCAATCTCGTATGCCGTC218610.1285216767819456TruSeq Adapter, Index 9 (97% over 34bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)