FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004691336

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691336
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40338066
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTGAAGAGAATCTCGTATGC490322012.155317510760185TruSeq Adapter, Index 2 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGAAGAGAATCTCGTATG418000610.362435323498156TruSeq Adapter, Index 2 (97% over 36bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTGAAGAGAAACTCGTATGC816100.20231510355503907TruSeq Adapter, Index 2 (97% over 35bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACTGAAGAGAATCTCGTATGCCG499390.12380117579261235TruSeq Adapter, Index 2 (96% over 33bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTGAAGAGAAGCTCGTATGC468920.11624751667568792TruSeq Adapter, Index 2 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGAAGAGAACCTCGTATG434900.1078137955349669TruSeq Adapter, Index 2 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA5085900.042.6921581
CGTATGC6769850.038.25502445
CGCCGTA12350.033.1489338
CGGTGGT124900.031.57859645
TCGCCGT14650.029.7871697
TGATACG175600.029.0152283
ACTCGTA164800.028.96796242
ATACGGC176000.028.7062685
GATACGG177650.028.5578214
AAACTCG165200.028.395640
TACGGCG176700.027.78966
AACTCGT171900.027.61468541
GTCGCCG17850.027.5975826
ACGGCGA180150.027.3073567
GATCGAA28000.026.8567851
CGGCGAC183200.026.6448
GGCGACC185450.026.041769
GCGACCA186950.025.72452510
CGACCAC187800.025.56167411
CCTCGTA172500.025.01435342
Produced by FastQC (version 0.11.5)