FastQCFastQC Report
Sat 11 May 2024
EGAF00004691346

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691346
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences65273506
Total Bases3.3 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAATGTTGCATCTCGTATG2452766337.576751277922774TruSeq Adapter, Index 25 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAATGTTGCATCTCGTAT48231937.389204741047616TruSeq Adapter, Index 25 (97% over 37bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAATGTTGCACCTCGTATG3059680.4687476110138775TruSeq Adapter, Index 25 (97% over 36bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAATGTTGCAACTCGTATG2612020.40016542086769474TruSeq Adapter, Index 25 (97% over 36bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAATGTTGCAGCTCGTATG1980380.30339721601594377TruSeq Adapter, Index 25 (97% over 36bp)
GAAGAGCACACGTCTGAACTCCAGTCACAATGTTGCATCTCGTATGCCGT1575670.24139503093337744TruSeq Adapter, Index 25 (96% over 32bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACAATGTTGCATCTCGTATGCC1060850.16252382704860377TruSeq Adapter, Index 25 (97% over 34bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAATGTTGCACCTCGTAT827400.1267589334024742TruSeq Adapter, Index 25 (97% over 37bp)

[OK]Adapter Content

Adapter graph