FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004691351

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691351
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16473414
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC17650.028.81039245
CTCGTAT19850.025.27678743
TCTCGTA19800.024.20309342
TCGTATG22550.022.44978544
ATCGGAA24250.021.7545551
ATCTCGT22750.021.55875241
CGTCTGA24150.021.23760815
TCGGAAG25050.021.022192
ACACGTC24500.020.56694812
CGGAAGA27900.018.8698923
ACGTCTG27900.018.22183614
GATCGGA10250.017.671451
CACGTCT30500.016.81600813
GCACACG34400.014.90949610
CACACGT35800.014.70345611
AATCTCG36050.013.91682740
GTCACAC46700.011.70643728
ACACCTC49500.010.54414931
CCAGTCA55550.09.71962225
CAGTCAC57350.09.6891526
Produced by FastQC (version 0.11.5)