FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004691355

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691355
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33773701
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC35800.030.60743345
GATCGGA35750.026.4110321
CTCGTAT53300.020.05113843
TCTCGTA51300.019.38446242
CGTATCA58850.018.8082434
ACGTATC59550.018.5117633
TCGTATG59200.018.3568244
ATCGGAA63450.017.4502871
CGTCTGA63900.017.24973115
ACACGTC64400.017.22059612
TACGGCG1701.8371793E-917.205164
ATCTCGT59550.016.88750841
TCGGAAG67250.016.631892
TCACACG68550.016.27843929
CGGAAGA71800.015.3858443
ACGTCTG72950.015.29478914
CACGTAT72300.015.21612232
ACACGTA74000.014.98817231
CACGTCT77300.014.49228913
GCACACG84800.013.34313810
Produced by FastQC (version 0.11.5)