FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004691358

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691358
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9625057
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGCTAACATCTCGTATGC149419115.523970403499948TruSeq Adapter, Index 13 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGCTAACATCTCGTATG6490246.743066560540887TruSeq Adapter, Index 13 (97% over 37bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACAGGCTAACATCTCGTATGCCG422160.43860519475365184TruSeq Adapter, Index 13 (97% over 34bp)
GAAGAGCACACGTCTGAACTCCAGTCACAGGCTAACATCTCGTATGCCGTC348960.36255369708459906TruSeq Adapter, Index 13 (96% over 32bp)
TCGGAAGAGCACACGTCTGAACTCCAGTCACAGGCTAACATCTCGTATGCC317250.3296084376435381TruSeq Adapter, Index 13 (97% over 35bp)
AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTT267130.2775360187477331Illumina Single End PCR Primer 1 (100% over 51bp)
GAAGAGCACACGTCTGAACTCCAGTCACAGGCTAACATCTCGTATGCCGTA196760.20442476340659593TruSeq Adapter, Index 13 (96% over 32bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGCTAACAACTCGTATGC174580.18138074403091847TruSeq Adapter, Index 13 (97% over 36bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGCTAACACCTCGTATGC154740.16076787908892384TruSeq Adapter, Index 13 (97% over 36bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGCCAACATCTCGTATGC117890.12248239153285015TruSeq Adapter, Index 13 (97% over 36bp)
NTCGGAAGAGCACACGTCTGAACTCCAGTCACAGGCTAACATCTCGTATGC102200.10618118936854089TruSeq Adapter, Index 13 (97% over 34bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAATGTTGCATCTCGTATGC100740.10466431523470458TruSeq Adapter, Index 25 (97% over 36bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGCTAACAGCTCGTATGC97660.1014643341852417TruSeq Adapter, Index 13 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGCCG6550.039.8382456
CGCCGTA6150.039.1374178
CGTATGC2074850.038.6762145
GGTCGCC6800.038.3728035
TCGCCGT6850.036.7799347
TTCGGAA8550.033.663331
TGGTCGC8650.033.0264634
GCTCGTA23300.032.15249642
AGCTCGT24750.030.90509441
GTGGTCG9800.030.783243
ACTCGTA34950.030.70418442
TGATACG74150.030.4830283
GATACGG74600.030.273974
CAGCTCG25500.029.81967440
ATACGGC75950.029.6173845
CGTACGC1150.029.36386545
CCTCGTA38700.029.00787542
TACGGCG75200.028.8065956
TGCCGTA32300.028.7850745
TCGTATG2828200.028.34189444
Produced by FastQC (version 0.11.5)